top of page
mmu_circCep192_249
Basic information of circular RNA
PanCircBase ID:
mmu_circCep192_249
mm39_Chr. Position ID:
chr18|67945396|67947070|+
Gene name:
Cep192
Transcript:
ENSMUST00000025425.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
7,8
Exon/intron offsets:
0,1546
Genomic length:
1674
Exon/intron sizes in nt:
1,21,128
Flanking exon/introns:
chr18:67943199-67945396|chr18:67947070-67948924
Splice length:
249
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATTGCCATGCCTGATCCTAAGAAGCATTTCGAAGCCGTGAATCCAAACCGTGATTTCAGCCATACCAGCTTATTAGACAATGAAAAACTGTTAACACTGACAAACTTGGACAGTTCCTCTGATGATGATATTAATGATGAAGAATTTTATGATGATCATTTAGAAGCATATTTTGAGCAACTAGCCATTCCAGGGATGATGTATGAAGCAGGAGGACAGGAGTCGCCAGCAGATGATTTTAAGTTCCCC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAACTAGCCATTCCAGGGA
59.74
TGGTATGGCTGAAATCACGGT
59.72
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAACTAGCCATTCCAGGGA
59.74
GCTGGTATGGCTGAAATCACG
59.67
142
PCR template sequence:
TGATGATCATTTAGAAGCATATTTTGAGCAACTAGCCATTCCAGGGATGATGTATGAAGCAGGAGGACAGGAGTCGCCAGCAGATGATTTTAAGTTCCCCATTGCCATGCCTGATCCTAAGAAGCATTTCGAAGCCGTGAATCCAAACCGTGATTTCAGCCATACCAGCTTATTAGACAATGAAAAACTGTTAACACTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-138-5p(1), mmu-miR-362-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTTAAGTTCCCCATTGCCATGCCTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEP192_249_ORF_1
1
0.236709067
0.754565697
492
ORF sequence:
ATGCCTGATCCTAAGAAGCATTTCGAAGCCGTGAATCCAAACCGTGATTTCAGCCATACCAGCTTATTAGACAATGAAAAACTGTTAACACTGACAAACTTGGACAGTTCCTCTGATGATGATATTAATGATGAAGAATTTTATGATGATCATTTAGAAGCATATTTTGAGCAACTAGCCATTCCAGGGATGATGTATGAAGCAGGAGGACAGGAGTCGCCAGCAGATGATTTTAAGTTCCCCATTGCCATGCCTGATCCTAAGAAGCATTTCGAAGCCGTGAATCCAAACCGTGATTTCAGCCATACCAGCTTATTAGACAATGAAAAACTGTTAACACTGACAAACTTGGACAGTTCCTCTGATGATGATATTAATGATGAAGAATTTTATGATGATCATTTAGAAGCATATTTTGAGCAACTAGCCATTCCAGGGATGATGTATGAAGCAGGAGGACAGGAGTCGCCAGCAGATGATTTTAAGTTCCCC
Peptide sequence:
MPDPKKHFEAVNPNRDFSHTSLLDNEKLLTLTNLDSSSDDDINDEEFYDDHLEAYFEQLAIPGMMYEAGGQESPADDFKFPIAMPDPKKHFEAVNPNRDFSHTSLLDNEKLLTLTNLDSSSDDDINDEEFYDDHLEAYFEQLAIPGMMYEAGGQESPADDFKFP
IRESfinder result
IRESfinder score
0.614230368
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page