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mmu_circCep135_658
Basic information of circular RNA
PanCircBase ID:
mmu_circCep135_658
mm39_Chr. Position ID:
chr5|76739561|76743381|+
Gene name:
Cep135
Transcript:
ENSMUST00000130651.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
2,3,4,5
Exon/intron offsets:
0,1477,2308,3678
Genomic length:
3820
Exon/intron sizes in nt:
1,57,19,11,68,142
Flanking exon/introns:
chr5:76736771-76739561|chr5:76743381-76745203
Splice length:
658
Other database IDs:
circAtlas: mmu-Cep135_0020
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_137382
Human Ortholog IDs:
Mature spliced Sequence:
GCCTTTAAGTTGGGAGTGTGACTAAAACTGGTTTTGGAAGCAAGATGACTACAGCTGCAGAGAGAAAGTATATTAACATTCGGAAGAGGTTAGACCAGCTGGGCTACCGCCAGACCCTGTCAGTGGACAGTCTGCCTTTGGTAGAAAAACTTTTCAGTGACCTCGTTCATACGACAGAAAGCCTGCGGCAGTGCAGGTTGTCCTCGGGAAAGGCGGAAAAGGAAAGTGCCAATCTCGATTTTGTTTTGGAACCTTATAAACTTGAAAATACAAGACTGAATAAGGAGAATAATGAATTGTATCTGGAGTTAATGAAGCTCAGAGAATGCTCAGACAAGCACATTAAAGACTTGAAGACGACACTGAAGAAATGTTCCCGTGAGACAGCTGATCTGAAGTTTCTGAACAACCAGTATGTTCACAAGGTCAAAGTCCTGGAGAAAGAGAGCAAAGCCAAGGATGAAAAGATCCAGCAGCTTCAAGAGAAGAACCTGCGTGCTGTGGTGCAGACCCCTGGTGGCAGGAAAAGAAACATTGCATTTAGGCGCCAGCGGATGCAAATCGATGAACCAGCCCCACCCTCAGAAGTCAGCGCATACCCGGTTCCTCAACCAGAAGACCCATACATTGCGGACCTCCTGCAAGTGGCTGACAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGAAGTCAGCGCATACCC
59.82
GCTGGTCTAACCTCTTCCGA
58.82
176
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGAAGTCAGCGCATACCC
59.82
AGCTGGTCTAACCTCTTCCG
58.81
177
PCR template sequence:
AAATCGATGAACCAGCCCCACCCTCAGAAGTCAGCGCATACCCGGTTCCTCAACCAGAAGACCCATACATTGCGGACCTCCTGCAAGTGGCTGACAACAGGCCTTTAAGTTGGGAGTGTGACTAAAACTGGTTTTGGAAGCAAGATGACTACAGCTGCAGAGAGAAAGTATATTAACATTCGGAAGAGGTTAGACCAGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-96-5p(1), mmu-miR-218-5p(1), mmu-miR-324-3p(1), mmu-miR-335-3p(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGCTGACAACAGGCCTTTAAGTTGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEP135_658_ORF_1
1.1117
0.28309004
0.928386372
639
ORF sequence:
ATGACTACAGCTGCAGAGAGAAAGTATATTAACATTCGGAAGAGGTTAGACCAGCTGGGCTACCGCCAGACCCTGTCAGTGGACAGTCTGCCTTTGGTAGAAAAACTTTTCAGTGACCTCGTTCATACGACAGAAAGCCTGCGGCAGTGCAGGTTGTCCTCGGGAAAGGCGGAAAAGGAAAGTGCCAATCTCGATTTTGTTTTGGAACCTTATAAACTTGAAAATACAAGACTGAATAAGGAGAATAATGAATTGTATCTGGAGTTAATGAAGCTCAGAGAATGCTCAGACAAGCACATTAAAGACTTGAAGACGACACTGAAGAAATGTTCCCGTGAGACAGCTGATCTGAAGTTTCTGAACAACCAGTATGTTCACAAGGTCAAAGTCCTGGAGAAAGAGAGCAAAGCCAAGGATGAAAAGATCCAGCAGCTTCAAGAGAAGAACCTGCGTGCTGTGGTGCAGACCCCTGGTGGCAGGAAAAGAAACATTGCATTTAGGCGCCAGCGGATGCAAATCGATGAACCAGCCCCACCCTCAGAAGTCAGCGCATACCCGGTTCCTCAACCAGAAGACCCATACATTGCGGACCTCCTGCAAGTGGCTGACAACAGGCCTTTAAGTTGGGAGTGTGACTAA
Peptide sequence:
MTTAAERKYINIRKRLDQLGYRQTLSVDSLPLVEKLFSDLVHTTESLRQCRLSSGKAEKESANLDFVLEPYKLENTRLNKENNELYLELMKLRECSDKHIKDLKTTLKKCSRETADLKFLNNQYVHKVKVLEKESKAKDEKIQQLQEKNLRAVVQTPGGRKRNIAFRRQRMQIDEPAPPSEVSAYPVPQPEDPYIADLLQVADNRPLSWECD
IRESfinder result
IRESfinder score
0.43341319
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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