top of page
mmu_circCep135_3072
Basic information of circular RNA
PanCircBase ID:
mmu_circCep135_3072
mm39_Chr. Position ID:
chr5|76778511|76788842|+
Gene name:
Cep135
Transcript:
ENSMUST00000130651.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
16,17,18,19,20,21,22,23
Exon/intron offsets:
0,188,760,1494,3008,3487,6197,10217
Genomic length:
10331
Exon/intron sizes in nt:
1,16,15,55,61,69,11,11,00,00,00,000
Flanking exon/introns:
chr5:76772625-76778511|chr5:76788842-76792478
Splice length:
3072
Other database IDs:
circBase: hsa_circ_0126660, circAtlas: hsa-CEP135_0062, CIRCpedia: HSA_CIRCpedia_158968
Human Ortholog IDs:
Mature spliced Sequence:
GATCGTCAGTGAGCAGCTACAGCGCTCACTTGATGACTGTCAGCACCGACTTTCCATAAAAAGAGGTGAACTTGAATCAGCCCAAGAGCAAATTAAAATGCTGGAGCAAAAACTAGAGAATCTCAGCCACAGGATGACGGTGCAGAGCGAAGAGACTCACGCCATGAAGAAGACCATTGGAGTGATGGACAAAGAAAAGGACTTTCTCCAGGAGACTGTGGATGAGAAGACAGAAAAGATCGCCAACCTGCAGGAAAGCCTCCTTAGTAAAGAAAAAGTCATTGCCCAGTTGAAGGTCACAGTTGCAGAGTATGAAACATCACTTAACCAGCTACAAGAAACTTTGACTACTCGAGACCGAGAGATAAACAGCCTCCGGCGCCAGCTTGATGCATCTCACAAGGAGCTTGATGATGTTGGGAAATCTAGAGAGATCTCTTTTAAGGAAAATAGAAGGTTACAAGATGACCTGGCCACAATGGCGAGAGAAAATCAGGAGATCTCACTGGAGCTGGAAGCAGCAGTGCAAGAAAAAGAAGAGATGAAGAGCAGAGTCCATAAGTACATCACTGAGGTGTCGCGGTGGGAGAGCCTGATGGCCGCCAAGGAAAAAGAAAACAAAGACTTGCTGGATAGATTCCAGATGCTCCACAGCCGTGCTGAGGACTGGGAGGTCAAGGCTCAGCAAGCGGAAGGGGAGAACAGCTCAGTCCGCCTGGAGCTGCTGTCTATCGACACAGAGAGAAGGCATCTGCGGGAACGAGTGGACCTCCTGGAGAAGGAGATCCAGGAGCACATCAATGCACATCACGCATACGAGTCTCAGATCTCGTCAATGGCTAAGGCCATGTCTCAGTTAGAAGAGGAGCTGCGACGTCATGAGAGCGAGAAAGCCACCATGTTAGGTGACGTGTCGTCTCTCCGAGAACTGTGCATCAAGCTCGACTCAGGCAAAGACGTCATGACCCAGCAGCTGAATTCCAAAAGCCTAGAGCTGGAGCGGGTAGGCGCTGAGATGCATTGGTGGGCGCTCACTCACGGCCTGACTTCACGTTCTCTTTTTGAGTGTTCCGTTTGTTCCCTTTGACTGCAAGTTTTTGATGACTGGGTTTGACCGCCTGGCTGCTGAGGTCAAACTCTAGGGAGCATGAGAATGAGATGGGGGTAGCTGAGCCGGGAGTCCTTGCCTTCCTGTGAGACACACTGGATTTGGTCTCAGCACTGCAAGAAAAGAAAAAGCAGATAAAATTGGGTTCGGAATAGATGGTACTTAAGAAGTTAGGCATTTTCTAGGTGTAGATCAGGAATATACAGTCTTTTTGTTTTTGTTTTTGTTTTTGTTTTTCAAGACAGGGTTTCTCTGTGTGTCCCTAGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCTCCTGCCTCTGCCTCCCGAGTGCTGGGATCAAAGGCATGCGCCACCACTGCCCAACTACATTCTTTTTTTATTTAAAATTAAATTTTGGTTTAAAGATTAAACAGTTTTCTAACTTGAATTTTTTAATAATGAAATCTCACGGCATTTACATTATTAATTAAGCCTTCTTTACTGAACTTGCCCATCCCCAAGCTGAGACTTTGACTGGCCTGGTGTGTGCTCTCGTGTGAGCATGGCTTTGGTTTCTCTTGTCAAGTCGTGCATTGAGAAGTTATAGCACTTGCTTGACTGTGGCCAGAGAACTGGCATGCTGCAGAGAGCATGGACTCATGGCTGATGGGGCGGGAGGCTTATCATATCCTTACCCTCTCATGATGAGGGAGATGGCTGGTCATCATCGGAGAGGCAGAGTACGCCTTAAAGCCAAGGGGACTTTAAATCACAATGATTTTCTTACAACTAAAAAAAAATGTTTTAAAGTCTTCAACAATCGCTGTTATATTGTAAGATAAAGAATTTTTCAGACTAAGCCTAAACTTTTGCTGAATTAATCTTTGTTTTTTCTGTAGGCGGTAGCAGAATTAGAGAATGTAAAATCAGAATCAGAACTATTAAAAAAACAGCTGACGAATGAGAGACAGACAATTAAAAACCTTGAGTCGTTGTTAGCTACAAACAGAGATAAAGAGTTCCAGTCCCATTTAACCTCCCACGAGAAGGACACAGAAATCCAGCTGCTGAAGGAGAAGCTAAATCTCTCAGAAAGCAAACTGTGAGTGTCGCTGAGCAGTGTGTGGGGAAGTGTCTTTGACAAAGTAGTTTCAACTTTCCAGGTAGCGAGGGCATTGGAAACCCACGGTCCCTGACTCTCCTAACTGCTGCGTCTGGACATCCAGAGGAGTCACTCAGGAGACATGGGGTTTGAAGCCGTAGACGTACACATCTTCATCCCCAAGGCAGGGCGTGGGATCGAGCCGGGAAGAGTGAGCTTCAGGGGAGGACCTGAGTCTGAGGAGTGAGTAAAGGCTTTGCTGAGGGGAGGCGTGTCCAGATGAGGTGGCTGTAGACAGGAAAGGCTTGGAGCGGGGGAGATTTGGTTCTCAAGTGCGTTGTTCATCACCATCAAGGGGACTGGTGATGACATCATCCCAGAAGAGAAGAGCCTGGACTTGCGAGCATGCAGAAAAATGGGCTTTGTACAGGGCTAGGGAAGTTGAGGCAAGAGGCAAGGAAGAACCAAGGAACCTGTGTTGCATCCTGTGTTGCTAGCTCATGACTGTGCAGAAGCTCTGGTTTGGAGAGAGCCCCTGTTGAAAAGCTGTGATCCTTGTCTGTTCTTAGGAATCAACCCATTTGTATCTGCACTTGATTCTACAAAAGAGGTCATTCGTTTTTCTATTTCTAAGATCTATACATTTGTTTTTAAGGACTACCCAAAGCCGAGAAACCTCCATGCTTCGAACTAAAGTGACACAGTTGCAGACAGATTATGATAATCTGAAGAGGCAGATGTCAAACGAGAAGTACGAACGAGAGCGAGCAATCCAAGAGATGCGCCGACTTGGTCTTCCTACATCACCTCTGAGCTCTACTCTGAAATCTCCTGTACAGACTCCTGATCATATAAATGCATAGTTACCAGGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGCCGACTTGGTCTTCCTAC
60.46
GTCGGTGCTGACAGTCATCA
60.04
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCGACTTGGTCTTCCTACA
59.39
GTCGGTGCTGACAGTCATCA
60.04
141
PCR template sequence:
AAGAGATGCGCCGACTTGGTCTTCCTACATCACCTCTGAGCTCTACTCTGAAATCTCCTGTACAGACTCCTGATCATATAAATGCATAGTTACCAGGAAGGATCGTCAGTGAGCAGCTACAGCGCTCACTTGATGACTGTCAGCACCGACTTTCCATAAAAAGAGGTGAACTTGAATCAGCCCAAGAGCAAATTAAAATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
22
Interacting miRNAs (#binding sites)
mmu-miR-484(2), mmu-let-7e-5p(1), mmu-miR-135a-5p(1), mmu-miR-335-5p(1), mmu-miR-148b-3p(1), mmu-miR-298-5p(1), mmu-miR-709(1), mmu-miR-185-5p(1), mmu-miR-495-3p(1), mmu-miR-191-5p(1), mmu-miR-127-5p(1), mmu-miR-297a-5p(1), mmu-miR-705(1), mmu-miR-342-3p(1), mmu-miR-1187(1), mmu-miR-1198-5p(1), mmu-miR-410-3p(1), mmu-miR-207(1), mmu-miR-532-5p(1), mmu-miR-452-3p(1), mmu-miR-1904(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGTTACCAGGAAGGATCGTCAGTGAG
siRNA sets
TCACTGACGATCCTTCCTG, CACTGACGATCCTTCCTGG, CTCACTGACGATCCTTCCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.58356343
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page