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mmu_circCep135_1154_2
Basic information of circular RNA
PanCircBase ID:
mmu_circCep135_1154_2
mm39_Chr. Position ID:
chr5|76743239|76763637|+
Gene name:
Cep135
Transcript:
ENSMUST00000130651.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
5,6,7,8,9,10,11,12
Exon/intron offsets:
0,1964,5994,7744,11329,14238,16257,20245
Genomic length:
20398
Exon/intron sizes in nt:
1,42,85,12,92,16,66,13,00,00,000
Flanking exon/introns:
chr5:76742037-76743239|chr5:76763637-76764649
Splice length:
1154
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGGCAGGAAAAGAAACATTGCATTTAGGCGCCAGCGGATGCAAATCGATGAACCAGCCCCACCCTCAGAAGTCAGCGCATACCCGGTTCCTCAACCAGAAGACCCATACATTGCGGACCTCCTGCAAGTGGCTGACAACAGGATTCAGGAGCTGCAGGAGGAGGTCCAGCAGCTGCAGGAGAAGCTAGCACAGATGGAGAAAGGAGTGCTGGACTACAGCAAGCAGATTGAACTGAGAGAACGAGAAATACAGCGACTGTCACTTGCGTTGGATGGCGGTTGTTCCCCTGATGTCCTGTCTCTGGAGACCAGAAATAAAACCAATGAGAAACTCATTGCTCACTTAAATGTCCAGGTTGACTTCCTCCAGCAAGCTAATAAAGAGCTGGAGAAGCATATTCAAGAGCTCATGGAAACCAAGGAAACAGTGACAACTGAAGTTGTGAACCTCAGCAACAGAAACGAGAAGCTCTGCCAGGAGCTAACCGAGATCGACCAGTTAGCCCAGCGGCTGGAAAGGCACAAGGAGCAAGTGCTGGAGACAGCAGACAAGGAGCTCGGGGAGGCAAAGAAAGAGATTAAACGAAACCTCTGTGAAATGCGGAACCTTGAGGAAAAAATGTCAAAACTGCAGTGGGAATTGGACTTAAGCCATAAGGAGAAGGAGAGACTGAACAGTGAGCTGCTTTTAAAGTCAGACCTGGAGACTGTTGTTCATCAGCTCGAACAAGAAAAGCAAAGACTTAGCAAAAAGCTGCAGAGTTTTGCAGTCACAGAAAGAGAACTGACTCTGGAGGTTGAGAGGATGAGGCTAGAACATGGGATAAAGCGTCGGGACAAGTCACCCTCTCGTCTGGACACATTTCTGAAAGGCATAGAGGAAGAGCGCGATTATTACAAGAAAGAGCTGGAGAAACTGCAGCATCTAATCCAGCGGAGATCTTGTGCGATTAATTACTCCGCTCGGGAAAAACCTCCTGTAGTCAAATGCTCAGAAAAGGGTGACTGCAGCACGGACGTTCACCTGATCACAAGAGAAAGGGATGAACTTCAGCGCATGCTGGAGAGATTCGAGAAATACATGGAGGACATACAGTCCAACGTGAAGCTGCTGACGGCGGAAAGGGACAAGCTGAATGTCCTGTATAAGGAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGCATGCTGGAGAGATTCGA
60.25
CATCGATTTGCATCCGCTGG
60.04
150
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGCATGCTGGAGAGATTCGA
60.25
GGGCTGGTTCATCGATTTGC
59.9
159
PCR template sequence:
GCGCATGCTGGAGAGATTCGAGAAATACATGGAGGACATACAGTCCAACGTGAAGCTGCTGACGGCGGAAAGGGACAAGCTGAATGTCCTGTATAAGGAAGTGGCAGGAAAAGAAACATTGCATTTAGGCGCCAGCGGATGCAAATCGATGAACCAGCCCCACCCTCAGAAGTCAGCGCATACCCGGTTCCTCAACCAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-207(4), mmu-miR-181a-5p(1), mmu-miR-135a-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-132-3p(1), mmu-miR-212-3p(1), mmu-miR-297a-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-129-5p(1), mmu-miR-452-3p(1), mmu-miR-338-3p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTGTATAAGGAAGTGGCAGGAAAAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEP135_1154_2_ORF_1
1.2617
0.330256751
0.999597279
1305
ORF sequence:
ATGCAAATCGATGAACCAGCCCCACCCTCAGAAGTCAGCGCATACCCGGTTCCTCAACCAGAAGACCCATACATTGCGGACCTCCTGCAAGTGGCTGACAACAGGATTCAGGAGCTGCAGGAGGAGGTCCAGCAGCTGCAGGAGAAGCTAGCACAGATGGAGAAAGGAGTGCTGGACTACAGCAAGCAGATTGAACTGAGAGAACGAGAAATACAGCGACTGTCACTTGCGTTGGATGGCGGTTGTTCCCCTGATGTCCTGTCTCTGGAGACCAGAAATAAAACCAATGAGAAACTCATTGCTCACTTAAATGTCCAGGTTGACTTCCTCCAGCAAGCTAATAAAGAGCTGGAGAAGCATATTCAAGAGCTCATGGAAACCAAGGAAACAGTGACAACTGAAGTTGTGAACCTCAGCAACAGAAACGAGAAGCTCTGCCAGGAGCTAACCGAGATCGACCAGTTAGCCCAGCGGCTGGAAAGGCACAAGGAGCAAGTGCTGGAGACAGCAGACAAGGAGCTCGGGGAGGCAAAGAAAGAGATTAAACGAAACCTCTGTGAAATGCGGAACCTTGAGGAAAAAATGTCAAAACTGCAGTGGGAATTGGACTTAAGCCATAAGGAGAAGGAGAGACTGAACAGTGAGCTGCTTTTAAAGTCAGACCTGGAGACTGTTGTTCATCAGCTCGAACAAGAAAAGCAAAGACTTAGCAAAAAGCTGCAGAGTTTTGCAGTCACAGAAAGAGAACTGACTCTGGAGGTTGAGAGGATGAGGCTAGAACATGGGATAAAGCGTCGGGACAAGTCACCCTCTCGTCTGGACACATTTCTGAAAGGCATAGAGGAAGAGCGCGATTATTACAAGAAAGAGCTGGAGAAACTGCAGCATCTAATCCAGCGGAGATCTTGTGCGATTAATTACTCCGCTCGGGAAAAACCTCCTGTAGTCAAATGCTCAGAAAAGGGTGACTGCAGCACGGACGTTCACCTGATCACAAGAGAAAGGGATGAACTTCAGCGCATGCTGGAGAGATTCGAGAAATACATGGAGGACATACAGTCCAACGTGAAGCTGCTGACGGCGGAAAGGGACAAGCTGAATGTCCTGTATAAGGAAGTGGCAGGAAAAGAAACATTGCATTTAGGCGCCAGCGGATGCAAATCGATGAACCAGCCCCACCCTCAGAAGTCAGCGCATACCCGGTTCCTCAACCAGAAGACCCATACATTGCGGACCTCCTGCAAGTGGCTGACAACAGGATTCAGGAGCTGCAGGAGGAGGTCCAGCAGCTGCAGGAGAAGCTAG
Peptide sequence:
MQIDEPAPPSEVSAYPVPQPEDPYIADLLQVADNRIQELQEEVQQLQEKLAQMEKGVLDYSKQIELREREIQRLSLALDGGCSPDVLSLETRNKTNEKLIAHLNVQVDFLQQANKELEKHIQELMETKETVTTEVVNLSNRNEKLCQELTEIDQLAQRLERHKEQVLETADKELGEAKKEIKRNLCEMRNLEEKMSKLQWELDLSHKEKERLNSELLLKSDLETVVHQLEQEKQRLSKKLQSFAVTERELTLEVERMRLEHGIKRRDKSPSRLDTFLKGIEEERDYYKKELEKLQHLIQRRSCAINYSAREKPPVVKCSEKGDCSTDVHLITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLNVLYKEVAGKETLHLGASGCKSMNQPHPQKSAHTRFLNQKTHTLRTSCKWLTTGFRSCRRRSSSCRRS
IRESfinder result
IRESfinder score
0.333788378
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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