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mmu_circCep120_414
Basic information of circular RNA
PanCircBase ID:
mmu_circCep120_414
mm39_Chr. Position ID:
chr18|53868714|53873280|-
Gene name:
Cep120
Transcript:
ENSMUST00000049811.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,4,3
Exon/intron offsets:
0,2835,4409
Genomic length:
4566
Exon/intron sizes in nt:
14,21,15,157
Flanking exon/introns:
chr18:53864311-53868714|chr18:53873280-53877353
Splice length:
414
Other database IDs:
CIRCpedia: MMU_CIRCpedia_228071, circAtlas: mmu-Cep120_0001
Human Ortholog IDs:
Mature spliced Sequence:
GTCGCCATTTCCCCAAACGTCCAAAGCATCTGCTCGTGGTGGAAGCAAAGTTTGATGGGGAGCAGCTGGCTACTGATCCTGTCGACCATACTGACCAGCCAGAATTTGCTACTGAGCTAGCCTGGGAGATTGACAGGAAAGTCCTTCATCAGCACCGGTTACAGCGCACGCCGATCAAACTCCAGTGCTTTGCCTTGGACCCGCAGACTTCTGCCAAGGAAACCGTAGGCTACATTGTTTTGGACTTAAGAACAGCTCAAGAAACAAAGCAGGCTCCCAAATGGTACCAGCTGCTGAGTAACAAATACACCAAGTTCAAGGCCGAGGTGCAGATCAGCCTCACTTTGGAGACAGATACAAAGGCCCAGGTGGATAGTTATAAAGCAAAAGCAGCCCCGCCTCGGGATGGAAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGCAGATCAGCCTCACTT
60.04
CGACAGGATCAGTAGCCAGC
60.25
173
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGCAGATCAGCCTCACTT
60.04
CAGCTGCTCCCCATCAAACT
60.32
157
PCR template sequence:
TTCAAGGCCGAGGTGCAGATCAGCCTCACTTTGGAGACAGATACAAAGGCCCAGGTGGATAGTTATAAAGCAAAAGCAGCCCCGCCTCGGGATGGAAAAGGTCGCCATTTCCCCAAACGTCCAAAGCATCTGCTCGTGGTGGAAGCAAAGTTTGATGGGGAGCAGCTGGCTACTGATCCTGTCGACCATACTGACCAGCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
CGGGATGGAAAAGGTCGCCATTTCCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEP120_414_ORF_1
1.2829
0.045566596
0.146853642
129
ORF sequence:
ATGGAAAAGGTCGCCATTTCCCCAAACGTCCAAAGCATCTGCTCGTGGTGGAAGCAAAGTTTGATGGGGAGCAGCTGGCTACTGATCCTGTCGACCATACTGACCAGCCAGAATTTGCTACTGAGCTAG
Peptide sequence:
MEKVAISPNVQSICSWWKQSLMGSSWLLILSTILTSQNLLLS
IRESfinder result
IRESfinder score
0.492645538
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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