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mmu_circCep112_654
Basic information of circular RNA
PanCircBase ID:
mmu_circCep112_654
mm39_Chr. Position ID:
chr11|108409504|108461249|+
Gene name:
Cep112
Transcript:
ENSMUST00000150863.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
13,14,15,16,17,18
Exon/intron offsets:
0,451,1217,4640,12707,51609
Genomic length:
51745
Exon/intron sizes in nt:
15,51,30,93,60,80,136
Flanking exon/introns:
chr11:108399191-108409504|chr11:108461249-108481829
Splice length:
654
Other database IDs:
CIRCpedia: MMU_CIRCpedia_2392, circAtlas: mmu-Cep112_0017
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_173831
Human Ortholog IDs:
Mature spliced Sequence:
AACCACATGATCAAAGAGCTGGAGGGCCGTGTCCAGCAACTGATGGGAGAAGCAGAGAACAGCAATCTACAGAGGCAGAAATTGACCCAAGAAAAATTGGAGCTGGAAAGATGCTACCAGATAACATGTAATGAATTGCAGGAGTTAAAGACAAGGCGCAACATACTACATAAAGAGAAAGAACATCTTGTAAATGACTATGAGCAAAATGTGAAATTATTAAAAACTAAATATGATTCTGACATAAATCTCCTGAGACAAGAGCATGCACTTTCAACTTCTAAGACATCTGGTGTGATTGAAGAGTTAGAACAGAACATCTGTCAATTAAAACAGCAGGTCCAAGAATCGGAGCTTCAGAGGAAACAGCAAGTAAAAGATCAAGAAAATAAGTTTCACATGGAGAAAAACCATTTAAAACACACCTATGAGAAAAAGGTTCATGAACTGCAGAGTGAACTTGATAAAGAGAAGGAAGATGCTCAGAGGAAGATCCATAAATTTGAGGAAGCTTTGAAGGAGAAGGAGGAGCAGCTCAGCAGAGTGACTGAAGTTCAGAGGCTGCAAGCCCAGCAGGCAGATGCTGCTCTGGAGGAGTTTAAGCGGCAGGTAGAAGTGAACTCTGAGAAGGTCTATGGTGAGATGAAAGAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGATGCTGCTCTGGAGGAGT
60.33
TGCTGTTCTCTGCTTCTCCC
59.68
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAGGAGTTTAAGCGGCAGGT
60.04
TGGGTCAATTTCTGCCTCTGT
59.57
152
PCR template sequence:
TCAGAGGCTGCAAGCCCAGCAGGCAGATGCTGCTCTGGAGGAGTTTAAGCGGCAGGTAGAAGTGAACTCTGAGAAGGTCTATGGTGAGATGAAAGAGCAGAACCACATGATCAAAGAGCTGGAGGGCCGTGTCCAGCAACTGATGGGAGAAGCAGAGAACAGCAATCTACAGAGGCAGAAATTGACCCAAGAAAAATTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-3470b(2), mmu-miR-140-5p(1), mmu-miR-93-5p(1), mmu-miR-103-3p(1), mmu-miR-322-5p(1), mmu-miR-127-5p(1), mmu-miR-370-3p(1), mmu-miR-107-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
GATGAAAGAGCAGAACCACATGATCA
siRNA sets
ATGTGGTTCTGCTCTTTCA, TGATCATGTGGTTCTGCTC, GATCATGTGGTTCTGCTCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEP112_654_ORF_1
1.1901
0.260803415
0.999390012
1302
ORF sequence:
ATGATCAAAGAGCTGGAGGGCCGTGTCCAGCAACTGATGGGAGAAGCAGAGAACAGCAATCTACAGAGGCAGAAATTGACCCAAGAAAAATTGGAGCTGGAAAGATGCTACCAGATAACATGTAATGAATTGCAGGAGTTAAAGACAAGGCGCAACATACTACATAAAGAGAAAGAACATCTTGTAAATGACTATGAGCAAAATGTGAAATTATTAAAAACTAAATATGATTCTGACATAAATCTCCTGAGACAAGAGCATGCACTTTCAACTTCTAAGACATCTGGTGTGATTGAAGAGTTAGAACAGAACATCTGTCAATTAAAACAGCAGGTCCAAGAATCGGAGCTTCAGAGGAAACAGCAAGTAAAAGATCAAGAAAATAAGTTTCACATGGAGAAAAACCATTTAAAACACACCTATGAGAAAAAGGTTCATGAACTGCAGAGTGAACTTGATAAAGAGAAGGAAGATGCTCAGAGGAAGATCCATAAATTTGAGGAAGCTTTGAAGGAGAAGGAGGAGCAGCTCAGCAGAGTGACTGAAGTTCAGAGGCTGCAAGCCCAGCAGGCAGATGCTGCTCTGGAGGAGTTTAAGCGGCAGGTAGAAGTGAACTCTGAGAAGGTCTATGGTGAGATGAAAGAGCAGAACCACATGATCAAAGAGCTGGAGGGCCGTGTCCAGCAACTGATGGGAGAAGCAGAGAACAGCAATCTACAGAGGCAGAAATTGACCCAAGAAAAATTGGAGCTGGAAAGATGCTACCAGATAACATGTAATGAATTGCAGGAGTTAAAGACAAGGCGCAACATACTACATAAAGAGAAAGAACATCTTGTAAATGACTATGAGCAAAATGTGAAATTATTAAAAACTAAATATGATTCTGACATAAATCTCCTGAGACAAGAGCATGCACTTTCAACTTCTAAGACATCTGGTGTGATTGAAGAGTTAGAACAGAACATCTGTCAATTAAAACAGCAGGTCCAAGAATCGGAGCTTCAGAGGAAACAGCAAGTAAAAGATCAAGAAAATAAGTTTCACATGGAGAAAAACCATTTAAAACACACCTATGAGAAAAAGGTTCATGAACTGCAGAGTGAACTTGATAAAGAGAAGGAAGATGCTCAGAGGAAGATCCATAAATTTGAGGAAGCTTTGAAGGAGAAGGAGGAGCAGCTCAGCAGAGTGACTGAAGTTCAGAGGCTGCAAGCCCAGCAGGCAGATGCTGCTCTGGAGGAGTTTAAGCGGCAGGTAGAAGTGAACTCTGAGAAGGTCTATGGTGAGATGAAAGAGCAG
Peptide sequence:
MIKELEGRVQQLMGEAENSNLQRQKLTQEKLELERCYQITCNELQELKTRRNILHKEKEHLVNDYEQNVKLLKTKYDSDINLLRQEHALSTSKTSGVIEELEQNICQLKQQVQESELQRKQQVKDQENKFHMEKNHLKHTYEKKVHELQSELDKEKEDAQRKIHKFEEALKEKEEQLSRVTEVQRLQAQQADAALEEFKRQVEVNSEKVYGEMKEQNHMIKELEGRVQQLMGEAENSNLQRQKLTQEKLELERCYQITCNELQELKTRRNILHKEKEHLVNDYEQNVKLLKTKYDSDINLLRQEHALSTSKTSGVIEELEQNICQLKQQVQESELQRKQQVKDQENKFHMEKNHLKHTYEKKVHELQSELDKEKEDAQRKIHKFEEALKEKEEQLSRVTEVQRLQAQQADAALEEFKRQVEVNSEKVYGEMKEQ
IRESfinder result
IRESfinder score
0.55133526
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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