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mmu_circCep112_427
Basic information of circular RNA
PanCircBase ID:
mmu_circCep112_427
mm39_Chr. Position ID:
chr11|108461113|108497221|+
Gene name:
Cep112
Transcript:
ENSMUST00000150863.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
18,19,20
Exon/intron offsets:
0,20716,35925
Genomic length:
36108
Exon/intron sizes in nt:
13,61,08,183
Flanking exon/introns:
chr11:108422291-108461113|chr11:108497221-108555321
Splice length:
427
Other database IDs:
CIRCpedia: MMU_CIRCpedia_2416, circAtlas: mmu-Cep112_0005
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_79573
Human Ortholog IDs:
Mature spliced Sequence:
GGAGAAGGAGGAGCAGCTCAGCAGAGTGACTGAAGTTCAGAGGCTGCAAGCCCAGCAGGCAGATGCTGCTCTGGAGGAGTTTAAGCGGCAGGTAGAAGTGAACTCTGAGAAGGTCTATGGTGAGATGAAAGAGCAGATGGAAAAAGTGGAAGCAGACCTAACGAGATCCAAGTCTCTCCGTGAGAAGCAGTCCAAGGAGTTTCTGTGGCAGCTGGAAGACGCCAAGCAGAGATATGAACAGCAGATAGTAGAGCTGAAGCTGGAGCATGAACAGGAGAAGACGCACCTATTACAGCAGCACAGCGCAGAGAAGGATAGCCTAGTCCGAGACCATGATCGGGAAATTGAAAACCTGGAGAAACAGCTTCGCGCTGCCAATATGGAGCACGAAAATCAAATTCAAGAGTCCAAGAAACGAGATGCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTGGAGAAACAGCTTCGC
60.6
ACCTGCCGCTTAAACTCCTC
60.04
170
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTGGAGAAACAGCTTCGC
60.6
TGCCGCTTAAACTCCTCCAG
60.04
167
PCR template sequence:
AGACCATGATCGGGAAATTGAAAACCTGGAGAAACAGCTTCGCGCTGCCAATATGGAGCACGAAAATCAAATTCAAGAGTCCAAGAAACGAGATGCTCAGGGAGAAGGAGGAGCAGCTCAGCAGAGTGACTGAAGTTCAGAGGCTGCAAGCCCAGCAGGCAGATGCTGCTCTGGAGGAGTTTAAGCGGCAGGTAGAAGTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-322-5p(1), mmu-miR-22-3p(1), mmu-miR-370-3p(1), mmu-miR-107-3p(1), mmu-miR-207(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
ACGAGATGCTCAGGGAGAAGGAGGAG
siRNA sets
CTCCTCCTTCTCCCTGAGC, TTCTCCCTGAGCATCTCGT, TCCTCCTTCTCCCTGAGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEP112_427_ORF_1
1.213
0.446048658
0.929778724
522
ORF sequence:
ATGATCGGGAAATTGAAAACCTGGAGAAACAGCTTCGCGCTGCCAATATGGAGCACGAAAATCAAATTCAAGAGTCCAAGAAACGAGATGCTCAGGGAGAAGGAGGAGCAGCTCAGCAGAGTGACTGAAGTTCAGAGGCTGCAAGCCCAGCAGGCAGATGCTGCTCTGGAGGAGTTTAAGCGGCAGGTAGAAGTGAACTCTGAGAAGGTCTATGGTGAGATGAAAGAGCAGATGGAAAAAGTGGAAGCAGACCTAACGAGATCCAAGTCTCTCCGTGAGAAGCAGTCCAAGGAGTTTCTGTGGCAGCTGGAAGACGCCAAGCAGAGATATGAACAGCAGATAGTAGAGCTGAAGCTGGAGCATGAACAGGAGAAGACGCACCTATTACAGCAGCACAGCGCAGAGAAGGATAGCCTAGTCCGAGACCATGATCGGGAAATTGAAAACCTGGAGAAACAGCTTCGCGCTGCCAATATGGAGCACGAAAATCAAATTCAAGAGTCCAAGAAACGAGATGCTCAG
Peptide sequence:
MIGKLKTWRNSFALPIWSTKIKFKSPRNEMLREKEEQLSRVTEVQRLQAQQADAALEEFKRQVEVNSEKVYGEMKEQMEKVEADLTRSKSLREKQSKEFLWQLEDAKQRYEQQIVELKLEHEQEKTHLLQQHSAEKDSLVRDHDREIENLEKQLRAANMEHENQIQESKKRDAQ
IRESfinder result
IRESfinder score
0.396313694
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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