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mmu_circCemip2_529
Basic information of circular RNA
PanCircBase ID:
mmu_circCemip2_529
mm39_Chr. Position ID:
chr19|21779225|21786802|+
Gene name:
Cemip2
Transcript:
ENSMUST00000096194.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
6,7,8
Exon/intron offsets:
0,5497,7407
Genomic length:
7577
Exon/intron sizes in nt:
17,01,89,170
Flanking exon/introns:
chr19:21775792-21779225|chr19:21786802-21789130
Splice length:
529
Other database IDs:
circAtlas: mmu-Tmem2_0004
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_111452
Human Ortholog IDs:
Mature spliced Sequence:
GCAAGCTTGGGCCTTAGTTGGTGTCATCGATGGTGGAAGTTCTTCCTGCAATGAATCTGTGAGAAACTATGAAAACCATAGCACTGGGGGGAAGGCCCTGGCTCAAGGAGAATTCTACACCCTGGACGGTCAGAAGTTCTCTGTGACAGCCTACAGTGAATGGAGTCAAGGAATCTCCCTCTCAGGCTTCCGGGTGGACATTGCGGATGGAGTGAAGCTTCACCTGTTGGACGACGTCAGCACTTGGGAAGCTGGAGACCGAATTGTGGTGGCCAGCACCGACTATTCCATGTACCAAGCAGAGGAGCTAACTCTTCTCCGCTGCCCCGAGTGCAGCCGTTCTCAGGTCAAAGTCAAAGAGATCCCCCAGTACCTGCACGTCGGTGAGATCATAGACGGGATAGACATGAGAGCTGAGGTTGGACTTCTCACCCGCAACATTGTGATCCAAGGAGAGATGGAGGATTCATGCTATGCAGAAAATCACTGCCAGTTCTTCGATTATGACACCTTTGGGGGACACGTCATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CACCCGCAACATTGTGATCC
59.83
ACTTCCACCATCGATGACACC
60.07
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CACCCGCAACATTGTGATCC
59.83
ACTTCCACCATCGATGACAC
57.61
140
PCR template sequence:
CACCCGCAACATTGTGATCCAAGGAGAGATGGAGGATTCATGCTATGCAGAAAATCACTGCCAGTTCTTCGATTATGACACCTTTGGGGGACACGTCATGGCAAGCTTGGGCCTTAGTTGGTGTCATCGATGGTGGAAGTTCTTCCTGCAATGAATCTGTGAGAAACTATGAAAACCATAGCACTGGGGGGAAGGCCCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(1), mmu-miR-34b-5p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGGACACGTCATGGCAAGCTTGGGCC
siRNA sets
AAGCTTGCCATGACGTGTC, CAAGCTTGCCATGACGTGT, GCTTGCCATGACGTGTCCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEMIP2_529_ORF_1
1.1024
0.343036248
0.917306751
585
ORF sequence:
ATGCAGAAAATCACTGCCAGTTCTTCGATTATGACACCTTTGGGGGACACGTCATGGCAAGCTTGGGCCTTAGTTGGTGTCATCGATGGTGGAAGTTCTTCCTGCAATGAATCTGTGAGAAACTATGAAAACCATAGCACTGGGGGGAAGGCCCTGGCTCAAGGAGAATTCTACACCCTGGACGGTCAGAAGTTCTCTGTGACAGCCTACAGTGAATGGAGTCAAGGAATCTCCCTCTCAGGCTTCCGGGTGGACATTGCGGATGGAGTGAAGCTTCACCTGTTGGACGACGTCAGCACTTGGGAAGCTGGAGACCGAATTGTGGTGGCCAGCACCGACTATTCCATGTACCAAGCAGAGGAGCTAACTCTTCTCCGCTGCCCCGAGTGCAGCCGTTCTCAGGTCAAAGTCAAAGAGATCCCCCAGTACCTGCACGTCGGTGAGATCATAGACGGGATAGACATGAGAGCTGAGGTTGGACTTCTCACCCGCAACATTGTGATCCAAGGAGAGATGGAGGATTCATGCTATGCAGAAAATCACTGCCAGTTCTTCGATTATGACACCTTTGGGGGACACGTCATG
Peptide sequence:
MQKITASSSIMTPLGDTSWQAWALVGVIDGGSSSCNESVRNYENHSTGGKALAQGEFYTLDGQKFSVTAYSEWSQGISLSGFRVDIADGVKLHLLDDVSTWEAGDRIVVASTDYSMYQAEELTLLRCPECSRSQVKVKEIPQYLHVGEIIDGIDMRAEVGLLTRNIVIQGEMEDSCYAENHCQFFDYDTFGGHVM
IRESfinder result
IRESfinder score
0.41155288
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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