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mmu_circCemip2_1951
Basic information of circular RNA
PanCircBase ID:
mmu_circCemip2_1951
mm39_Chr. Position ID:
chr19|21779225|21809606|+
Gene name:
Cemip2
Transcript:
ENSMUST00000096194.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
6,7,8,9,10,11,12,13,14,15,16,17,18
Exon/intron offsets:
0,5497,7407,9905,10349,10689,13569,16061,21926,23228,24178,27938,30165
Genomic length:
30381
Exon/intron sizes in nt:
1,70,18,91,70,21,02,06,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr19:21775792-21779225|chr19:21809606-21812781
Splice length:
1951
Other database IDs:
CIRCpedia: MMU_CIRCpedia_13385, circAtlas: mmu-Tmem2_0005
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_111442
Human Ortholog IDs:
Mature spliced Sequence:
GCAAGCTTGGGCCTTAGTTGGTGTCATCGATGGTGGAAGTTCTTCCTGCAATGAATCTGTGAGAAACTATGAAAACCATAGCACTGGGGGGAAGGCCCTGGCTCAAGGAGAATTCTACACCCTGGACGGTCAGAAGTTCTCTGTGACAGCCTACAGTGAATGGAGTCAAGGAATCTCCCTCTCAGGCTTCCGGGTGGACATTGCGGATGGAGTGAAGCTTCACCTGTTGGACGACGTCAGCACTTGGGAAGCTGGAGACCGAATTGTGGTGGCCAGCACCGACTATTCCATGTACCAAGCAGAGGAGCTAACTCTTCTCCGCTGCCCCGAGTGCAGCCGTTCTCAGGTCAAAGTCAAAGAGATCCCCCAGTACCTGCACGTCGGTGAGATCATAGACGGGATAGACATGAGAGCTGAGGTTGGACTTCTCACCCGCAACATTGTGATCCAAGGAGAGATGGAGGATTCATGCTATGCAGAAAATCACTGCCAGTTCTTCGATTATGACACCTTTGGGGGACACGTCATGATAGAGAAGAATTTTACTTCAGTCCATCTTTCTTATGTGGAATTGAAACACATGGGCCAGCAGCATATGGGCCGGTATCCTGTGCATTTTCACCTGTGTGGAGATGTAGATTCTAAAGGAGGGTACAGCCAGCCAGCGTCTGTGGATGGCCTGTCTGTTCATCACAGCTTTTCAAGGTGCATCACCGTGCACGGGACCAGTGGCTTGCTAATCAAAGACACCATTGGCTTCGACACACTAGGTCATTGCTTCTTTTTGGAAGATGGTGTTGAACAGCGAAATATTTTGTATCATAACTTGGGGCTTCTTACCAAGCCGGGCACTCTCCTCCCAACGGACAGGAACAGCTCCATGTGCACCGTCATGCGTGATGGAGTGTTTGGAAACTACGTCCCTGTGCCTACCACAGACTGCATGGCTGTTTCAACTTTCTGGATTGCCCACCCCAACAACCACCTGATCAATAACGCAGCTGCAGGCTCACAGGATGCTGGAATATGGTATTTATTCCACAAGGAACCAACTGGGGAGTCCAGTGGGCTGCAGCTCCTGGAGAAGCCAGAACTCACTCCGCTGGGGATATTTTATAACAACAGGGTTCATTCAAATTTTAAGGCTGGTTTATTTGTGGACAAAGGTGTCAAAACGACCAACGCCAGTGCATCTGACCCAAGGGAGTACCTCTGCCTGGATAACAGTGCAAGGTTTCGGCCCCATCAGGATGCAGACCCTGAGAAGCCACGCGTTGCAGCCATCATCGACAGGCTCATTGCTTTTAAGAACAATGACAACGGAGCCTGGGTCAGAGGAGGAGATATTATTGTTCAGAATTCAGCATTTGCAGATAACGGAAAGGGGCTGACCTTTGCCAGTGATGGGAGCTTCCCAAGCGATGAAGGGTCCAGTCAGGAGGTGACAGAGTCCCTCTTTGTCGGGGAGAGTAGGAATTACGGCTTTCAAGGTGGCCAAAACAAGTACATGGGCACAGGGGGAATCGATCAGAAGCCTCGGACGCTACCCAGGAACAGGACATTCCCAATCCGAGGCTTTCAGATTTACGATGGGCCTATTCATCTCACAAAGAGTACTTTCAAAAAGTATGTGCCAACTCCAGACAGGTACAGCAGTGCAATTGGCTTCCTCATGAAGAATTCCTGGCAGACAACACCCAGGAATAACGTTTCCCTTGTGAAGTTTGGTCCACAGGTCTCTCTGAACGTCTTCTTTGGAAAGCCTGGCCCCTGGTTTGAGGATTGTGAGCTGGACGGCGATAAGAACTCCATCTTCCATGACATTGATGGCTCTGTGACAGGATACAAGGACACCTATGTGGGAAGGATGGACAACTACCTGATCCGCCATCCAAACTGTGTCAATGTTACTAAGTGGAATGCAGTGATCTGTAGCGGGACCTACGCCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATCCGCCATCCAAACTGTGT
59.96
CCCCCAGTGCTATGGTTTTCA
60.27
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTGATCCGCCATCCAAACT
60.11
CCCCCAGTGCTATGGTTTTCA
60.27
163
PCR template sequence:
CACCTATGTGGGAAGGATGGACAACTACCTGATCCGCCATCCAAACTGTGTCAATGTTACTAAGTGGAATGCAGTGATCTGTAGCGGGACCTACGCCCAGGCAAGCTTGGGCCTTAGTTGGTGTCATCGATGGTGGAAGTTCTTCCTGCAATGAATCTGTGAGAAACTATGAAAACCATAGCACTGGGGGGAAGGCCCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
21
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-20b-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-92a-3p(1), mmu-miR-205-5p(1), mmu-miR-196a-5p(1), mmu-miR-320-3p(1), mmu-miR-25-3p(1), mmu-miR-10a-5p(1), mmu-miR-136-5p(1), mmu-miR-363-3p(1), mmu-miR-501-3p(1), mmu-miR-96-5p(1), mmu-miR-192-5p(1), mmu-miR-215-5p(1), mmu-miR-34b-5p(1), mmu-miR-207(1), mmu-miR-335-3p(1), mmu-miR-188-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GACCTACGCCCAGGCAAGCTTGGGCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEMIP2_1951_ORF_1
1.0242
0.23139743
0.999949811
1716
ORF sequence:
ATGTACCAAGCAGAGGAGCTAACTCTTCTCCGCTGCCCCGAGTGCAGCCGTTCTCAGGTCAAAGTCAAAGAGATCCCCCAGTACCTGCACGTCGGTGAGATCATAGACGGGATAGACATGAGAGCTGAGGTTGGACTTCTCACCCGCAACATTGTGATCCAAGGAGAGATGGAGGATTCATGCTATGCAGAAAATCACTGCCAGTTCTTCGATTATGACACCTTTGGGGGACACGTCATGATAGAGAAGAATTTTACTTCAGTCCATCTTTCTTATGTGGAATTGAAACACATGGGCCAGCAGCATATGGGCCGGTATCCTGTGCATTTTCACCTGTGTGGAGATGTAGATTCTAAAGGAGGGTACAGCCAGCCAGCGTCTGTGGATGGCCTGTCTGTTCATCACAGCTTTTCAAGGTGCATCACCGTGCACGGGACCAGTGGCTTGCTAATCAAAGACACCATTGGCTTCGACACACTAGGTCATTGCTTCTTTTTGGAAGATGGTGTTGAACAGCGAAATATTTTGTATCATAACTTGGGGCTTCTTACCAAGCCGGGCACTCTCCTCCCAACGGACAGGAACAGCTCCATGTGCACCGTCATGCGTGATGGAGTGTTTGGAAACTACGTCCCTGTGCCTACCACAGACTGCATGGCTGTTTCAACTTTCTGGATTGCCCACCCCAACAACCACCTGATCAATAACGCAGCTGCAGGCTCACAGGATGCTGGAATATGGTATTTATTCCACAAGGAACCAACTGGGGAGTCCAGTGGGCTGCAGCTCCTGGAGAAGCCAGAACTCACTCCGCTGGGGATATTTTATAACAACAGGGTTCATTCAAATTTTAAGGCTGGTTTATTTGTGGACAAAGGTGTCAAAACGACCAACGCCAGTGCATCTGACCCAAGGGAGTACCTCTGCCTGGATAACAGTGCAAGGTTTCGGCCCCATCAGGATGCAGACCCTGAGAAGCCACGCGTTGCAGCCATCATCGACAGGCTCATTGCTTTTAAGAACAATGACAACGGAGCCTGGGTCAGAGGAGGAGATATTATTGTTCAGAATTCAGCATTTGCAGATAACGGAAAGGGGCTGACCTTTGCCAGTGATGGGAGCTTCCCAAGCGATGAAGGGTCCAGTCAGGAGGTGACAGAGTCCCTCTTTGTCGGGGAGAGTAGGAATTACGGCTTTCAAGGTGGCCAAAACAAGTACATGGGCACAGGGGGAATCGATCAGAAGCCTCGGACGCTACCCAGGAACAGGACATTCCCAATCCGAGGCTTTCAGATTTACGATGGGCCTATTCATCTCACAAAGAGTACTTTCAAAAAGTATGTGCCAACTCCAGACAGGTACAGCAGTGCAATTGGCTTCCTCATGAAGAATTCCTGGCAGACAACACCCAGGAATAACGTTTCCCTTGTGAAGTTTGGTCCACAGGTCTCTCTGAACGTCTTCTTTGGAAAGCCTGGCCCCTGGTTTGAGGATTGTGAGCTGGACGGCGATAAGAACTCCATCTTCCATGACATTGATGGCTCTGTGACAGGATACAAGGACACCTATGTGGGAAGGATGGACAACTACCTGATCCGCCATCCAAACTGTGTCAATGTTACTAAGTGGAATGCAGTGATCTGTAGCGGGACCTACGCCCAGGCAAGCTTGGGCCTTAGTTGGTGTCATCGATGGTGGAAGTTCTTCCTGCAATGA
Peptide sequence:
MYQAEELTLLRCPECSRSQVKVKEIPQYLHVGEIIDGIDMRAEVGLLTRNIVIQGEMEDSCYAENHCQFFDYDTFGGHVMIEKNFTSVHLSYVELKHMGQQHMGRYPVHFHLCGDVDSKGGYSQPASVDGLSVHHSFSRCITVHGTSGLLIKDTIGFDTLGHCFFLEDGVEQRNILYHNLGLLTKPGTLLPTDRNSSMCTVMRDGVFGNYVPVPTTDCMAVSTFWIAHPNNHLINNAAAGSQDAGIWYLFHKEPTGESSGLQLLEKPELTPLGIFYNNRVHSNFKAGLFVDKGVKTTNASASDPREYLCLDNSARFRPHQDADPEKPRVAAIIDRLIAFKNNDNGAWVRGGDIIVQNSAFADNGKGLTFASDGSFPSDEGSSQEVTESLFVGESRNYGFQGGQNKYMGTGGIDQKPRTLPRNRTFPIRGFQIYDGPIHLTKSTFKKYVPTPDRYSSAIGFLMKNSWQTTPRNNVSLVKFGPQVSLNVFFGKPGPWFEDCELDGDKNSIFHDIDGSVTGYKDTYVGRMDNYLIRHPNCVNVTKWNAVICSGTYAQASLGLSWCHRWWKFFLQ
IRESfinder result
IRESfinder score
0.561673494
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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