top of page
mmu_circCelf2_184
Basic information of circular RNA
PanCircBase ID:
mmu_circCelf2_184
mm39_Chr. Position ID:
chr2|6620498|6629530|-
Gene name:
Celf2
Transcript:
ENSMUST00000002176.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
7,6
Exon/intron offsets:
0,8983
Genomic length:
9032
Exon/intron sizes in nt:
135,49
Flanking exon/introns:
chr2:6612772-6620498|chr2:6629530-6668925
Splice length:
184
Other database IDs:
circBase: mmu_circ_0010098, CIRCpedia: MMU_CIRCpedia_26450, circAtlas: mmu-Celf2_0024
circBase: hsa_circ_0092677, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_167168
Human Ortholog IDs:
Mature spliced Sequence:
ATGCATCATCCCATTCAGATGAAACCTGCAGATAGTGAAAAGTCAAACGCTGTGGAAGACAGAAAATTGTTCATAGGAATGGTTTCCAAGAAATGTAACGAGAATGATATCAGAGTGATGTTTTCTCCATTCGGTCAGATAGAAGAATGCCGGATTCTCCGGGGACCTGATGGGCTGAGTCGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGATGTTTTCTCCATTCGGTCA
59.25
TGTCTTCCACAGCGTTTGACT
60.13
131
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTTTTCTCCATTCGGTCAGA
57.24
TGTCTTCCACAGCGTTTGACT
60.13
127
PCR template sequence:
ATGTAACGAGAATGATATCAGAGTGATGTTTTCTCCATTCGGTCAGATAGAAGAATGCCGGATTCTCCGGGGACCTGATGGGCTGAGTCGAGATGCATCATCCCATTCAGATGAAACCTGCAGATAGTGAAAAGTCAAACGCTGTGGAAGACAGAAAATTGTTCATAGGAATGGTTTCCAAGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-24-3p(1), mmu-miR-434-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGGCTGAGTCGAGATGCATCATCCCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCELF2_184_ORF_1
0.6435
0.120045508
0.12090553
219
ORF sequence:
ATGCATCATCCCATTCAGATGAAACCTGCAGATAGTGAAAAGTCAAACGCTGTGGAAGACAGAAAATTGTTCATAGGAATGGTTTCCAAGAAATGTAACGAGAATGATATCAGAGTGATGTTTTCTCCATTCGGTCAGATAGAAGAATGCCGGATTCTCCGGGGACCTGATGGGCTGAGTCGAGATGCATCATCCCATTCAGATGAAACCTGCAGATAG
Peptide sequence:
MHHPIQMKPADSEKSNAVEDRKLFIGMVSKKCNENDIRVMFSPFGQIEECRILRGPDGLSRDASSHSDETCR
IRESfinder result
IRESfinder score
0.327143723
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page