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mmu_circCebpz_801
Basic information of circular RNA
PanCircBase ID:
mmu_circCebpz_801
mm39_Chr. Position ID:
chr17|79231874|79239770|-
Gene name:
Cebpz
Transcript:
ENSMUST00000024885.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
9,8,7,6,5,4,3
Exon/intron offsets:
0,1468,1637,2423,4832,6864,7664
Genomic length:
7896
Exon/intron sizes in nt:
67,69,10,35,48,91,87,200
Flanking exon/introns:
chr17:79230765-79231874|chr17:79239770-79242003
Splice length:
801
Other database IDs:
circAtlas: mmu-Cebpz_0001
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
AAAGATGTTGGATCCAGGCCTGACGACATGTTCCAAGCAAGCGATGTTTCTCAACCTGATCTACAAATCTTTGAAAGCTGACATCATGCTGCGCCGGGTGAAGGCGTTTGTGAAGAGGTTGCTTCAGGTCACCTGCACACAGATGCCGCCATTCATTTGTGGAGCGTTGTATCTTGTGTCTGAGATCCTTAAAGCAAAACCAGATTTAAGAAGCCAACTAGATGATCATCCGGAGTCTGACGAAGAAAACTTCGTTGACGTGGGAGATGACAGTGACGATGAAAAGTTTACCGATGCAGACAAAGAAACAGCAACAGATGCAGTAAAGGAAGTAGAGTCAAAGGAGACAGAGCCTGAGAGCAGCGCAGAGGCCGAGAAGCCGAAGGCCGCTTCCTGGGTGCACTTTGACAACTTGAAAGGTGGCAAGCAAATAAAGACATATGATCCATTCAGTAGAAATCCTTTATTCTGCGGAGCTGAAAATACAACTCTTTGGGAGCTCAAAAAGCTATCTGAACATTTCCATCCTTCTGTGGCCCTTTTTGCAAAGACTATCCTTGAGGGAAATTGTATTCAATACTCAGGGGACCCACTGCAGGATTTCACACTCATGAGATTCTTGGATCGATTTGTGTACCGAAATCCAAAGCTACATAAAGGCAAAGAAAATACCGACAGTGTTGTGATGCAACCCAAAAGGAAGCACTTTATGAAGAATGTCAGAGATCTCGCTGTGAACAGCAAGGAGTTCCTTGCAAAAGAAGAAAGCCAGATCCCAGTGGATGAAGTGTTCTTCTATAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCAGAGATCTCGCTGTGA
58.17
CGCAGCATGATGTCAGCTTT
59.55
177
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGTGAACAGCAAGGAGTTCC
59.12
CGCAGCATGATGTCAGCTTT
59.55
163
PCR template sequence:
AGCACTTTATGAAGAATGTCAGAGATCTCGCTGTGAACAGCAAGGAGTTCCTTGCAAAAGAAGAAAGCCAGATCCCAGTGGATGAAGTGTTCTTCTATAGAAAGATGTTGGATCCAGGCCTGACGACATGTTCCAAGCAAGCGATGTTTCTCAACCTGATCTACAAATCTTTGAAAGCTGACATCATGCTGCGCCGGGTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-17-5p(2), mmu-miR-20b-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-217-5p(1), mmu-miR-382-5p(1), mmu-miR-673-5p(1), mmu-miR-335-3p(1), mmu-miR-484(1), mmu-miR-350-3p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTTCTTCTATAGAAAGATGTTGGAT
siRNA sets
ATCCAACATCTTTCTATAG, CAACATCTTTCTATAGAAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCEBPZ_801_ORF_1
1.0587
0.240461513
0.999897818
1596
ORF sequence:
ATGTTGGATCCAGGCCTGACGACATGTTCCAAGCAAGCGATGTTTCTCAACCTGATCTACAAATCTTTGAAAGCTGACATCATGCTGCGCCGGGTGAAGGCGTTTGTGAAGAGGTTGCTTCAGGTCACCTGCACACAGATGCCGCCATTCATTTGTGGAGCGTTGTATCTTGTGTCTGAGATCCTTAAAGCAAAACCAGATTTAAGAAGCCAACTAGATGATCATCCGGAGTCTGACGAAGAAAACTTCGTTGACGTGGGAGATGACAGTGACGATGAAAAGTTTACCGATGCAGACAAAGAAACAGCAACAGATGCAGTAAAGGAAGTAGAGTCAAAGGAGACAGAGCCTGAGAGCAGCGCAGAGGCCGAGAAGCCGAAGGCCGCTTCCTGGGTGCACTTTGACAACTTGAAAGGTGGCAAGCAAATAAAGACATATGATCCATTCAGTAGAAATCCTTTATTCTGCGGAGCTGAAAATACAACTCTTTGGGAGCTCAAAAAGCTATCTGAACATTTCCATCCTTCTGTGGCCCTTTTTGCAAAGACTATCCTTGAGGGAAATTGTATTCAATACTCAGGGGACCCACTGCAGGATTTCACACTCATGAGATTCTTGGATCGATTTGTGTACCGAAATCCAAAGCTACATAAAGGCAAAGAAAATACCGACAGTGTTGTGATGCAACCCAAAAGGAAGCACTTTATGAAGAATGTCAGAGATCTCGCTGTGAACAGCAAGGAGTTCCTTGCAAAAGAAGAAAGCCAGATCCCAGTGGATGAAGTGTTCTTCTATAGAAAGATGTTGGATCCAGGCCTGACGACATGTTCCAAGCAAGCGATGTTTCTCAACCTGATCTACAAATCTTTGAAAGCTGACATCATGCTGCGCCGGGTGAAGGCGTTTGTGAAGAGGTTGCTTCAGGTCACCTGCACACAGATGCCGCCATTCATTTGTGGAGCGTTGTATCTTGTGTCTGAGATCCTTAAAGCAAAACCAGATTTAAGAAGCCAACTAGATGATCATCCGGAGTCTGACGAAGAAAACTTCGTTGACGTGGGAGATGACAGTGACGATGAAAAGTTTACCGATGCAGACAAAGAAACAGCAACAGATGCAGTAAAGGAAGTAGAGTCAAAGGAGACAGAGCCTGAGAGCAGCGCAGAGGCCGAGAAGCCGAAGGCCGCTTCCTGGGTGCACTTTGACAACTTGAAAGGTGGCAAGCAAATAAAGACATATGATCCATTCAGTAGAAATCCTTTATTCTGCGGAGCTGAAAATACAACTCTTTGGGAGCTCAAAAAGCTATCTGAACATTTCCATCCTTCTGTGGCCCTTTTTGCAAAGACTATCCTTGAGGGAAATTGTATTCAATACTCAGGGGACCCACTGCAGGATTTCACACTCATGAGATTCTTGGATCGATTTGTGTACCGAAATCCAAAGCTACATAAAGGCAAAGAAAATACCGACAGTGTTGTGATGCAACCCAAAAGGAAGCACTTTATGAAGAATGTCAGAGATCTCGCTGTGAACAGCAAGGAGTTCCTTGCAAAAGAAGAAAGCCAGATCCCAGTGGATGAAGTGTTCTTCTAT
Peptide sequence:
MLDPGLTTCSKQAMFLNLIYKSLKADIMLRRVKAFVKRLLQVTCTQMPPFICGALYLVSEILKAKPDLRSQLDDHPESDEENFVDVGDDSDDEKFTDADKETATDAVKEVESKETEPESSAEAEKPKAASWVHFDNLKGGKQIKTYDPFSRNPLFCGAENTTLWELKKLSEHFHPSVALFAKTILEGNCIQYSGDPLQDFTLMRFLDRFVYRNPKLHKGKENTDSVVMQPKRKHFMKNVRDLAVNSKEFLAKEESQIPVDEVFFYRKMLDPGLTTCSKQAMFLNLIYKSLKADIMLRRVKAFVKRLLQVTCTQMPPFICGALYLVSEILKAKPDLRSQLDDHPESDEENFVDVGDDSDDEKFTDADKETATDAVKEVESKETEPESSAEAEKPKAASWVHFDNLKGGKQIKTYDPFSRNPLFCGAENTTLWELKKLSEHFHPSVALFAKTILEGNCIQYSGDPLQDFTLMRFLDRFVYRNPKLHKGKENTDSVVMQPKRKHFMKNVRDLAVNSKEFLAKEESQIPVDEVFFY
IRESfinder result
IRESfinder score
0.476456091
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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