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mmu_circCdk14_279
Basic information of circular RNA
PanCircBase ID:
mmu_circCdk14_279
mm39_Chr. Position ID:
chr5|4999531|5086546|-
Gene name:
Cdk14
Transcript:
ENSMUST00000115451.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
10,9,8
Exon/intron offsets:
0,61301,86894
Genomic length:
87015
Exon/intron sizes in nt:
64,94,121
Flanking exon/introns:
chr5:4972175-4999531|chr5:5086546-5142957
Splice length:
279
Other database IDs:
circBase: hsa_circ_0003612, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_59248
Human Ortholog IDs:
Mature spliced Sequence:
GTCTGGCAAGAGCAAAATCCGTCCCTAGCCACACATACTCCAATGAAGTGGTTACCTTGTGGTACAGACCTCCAGATGTTCTTCTGGGCTCTACAGAATATTCCACCTGCCTTGACATGTGGGGAGTTGGCTGTATCTTCGTTGAGATGATCCAAGGAGTTGCTGCGTTTCCAGGAATGAAAGACATTCAGGATCAACTTGAACGGATATTTCTGGTTCTTGGAACACCGAATGAGGACACGTGGCCTGGAGTTCATTCTTTACCACATTTTAAGCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACACCGAATGAGGACACGTG
60.32
TCTGTAGAGCCCAGAAGAACA
58.11
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACACCGAATGAGGACACGT
59.61
TCTGTAGAGCCCAGAAGAACA
58.11
153
PCR template sequence:
AAAGACATTCAGGATCAACTTGAACGGATATTTCTGGTTCTTGGAACACCGAATGAGGACACGTGGCCTGGAGTTCATTCTTTACCACATTTTAAGCCAGGTCTGGCAAGAGCAAAATCCGTCCCTAGCCACACATACTCCAATGAAGTGGTTACCTTGTGGTACAGACCTCCAGATGTTCTTCTGGGCTCTACAGAATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CATTTTAAGCCAGGTCTGGCAAGAGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCDK14_279_ORF_1
0.8829
0.031227891
0.424529061
441
ORF sequence:
ATGTGGGGAGTTGGCTGTATCTTCGTTGAGATGATCCAAGGAGTTGCTGCGTTTCCAGGAATGAAAGACATTCAGGATCAACTTGAACGGATATTTCTGGTTCTTGGAACACCGAATGAGGACACGTGGCCTGGAGTTCATTCTTTACCACATTTTAAGCCAGGTCTGGCAAGAGCAAAATCCGTCCCTAGCCACACATACTCCAATGAAGTGGTTACCTTGTGGTACAGACCTCCAGATGTTCTTCTGGGCTCTACAGAATATTCCACCTGCCTTGACATGTGGGGAGTTGGCTGTATCTTCGTTGAGATGATCCAAGGAGTTGCTGCGTTTCCAGGAATGAAAGACATTCAGGATCAACTTGAACGGATATTTCTGGTTCTTGGAACACCGAATGAGGACACGTGGCCTGGAGTTCATTCTTTACCACATTTTAAGCCA
Peptide sequence:
MWGVGCIFVEMIQGVAAFPGMKDIQDQLERIFLVLGTPNEDTWPGVHSLPHFKPGLARAKSVPSHTYSNEVVTLWYRPPDVLLGSTEYSTCLDMWGVGCIFVEMIQGVAAFPGMKDIQDQLERIFLVLGTPNEDTWPGVHSLPHFKP
IRESfinder result
IRESfinder score
0.783601798
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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