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mmu_circCcz1_953
Basic information of circular RNA
PanCircBase ID:
mmu_circCcz1_953
mm39_Chr. Position ID:
chr5|143928289|143948347|-
Gene name:
Ccz1
Transcript:
ENSMUST00000031621.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
13,12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,1037,1754,6691,7072,9905,12571,17731,19559,19980
Genomic length:
20058
Exon/intron sizes in nt:
159,118,34,112,62,82,176,84,48,78
Flanking exon/introns:
chr5:143927583-143928289|chr5:143948347-143949620
Splice length:
953
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTGTTCGGAATCCTATCATCGAGAAGCAAAGTAAAGATGGCAAGGCGGTAGTGGAGTACCAAGAGGAGGAGCTGTTGGACAAGGTGTACAGCTCAGTGCTGCAGCAGTGCTACAGCATGTACAAGCTTTTCAATGGGACGTTTCTGAAAGCCATGGAAGATGGAGGCGTCAAGCTCCTAAAGGAAAGATTAGAGAAATTCTTCCATCGGTATTTGCAGACACTACATTTGCAGTCATGTGACCTACTTGATATTTTTGGTGGAATCAGCTTCTTCCCATTGGATAAAATGACTTACTTGAAAATCCAGTCCTTTATTAACCGAATGGAAGAAAGCCTAAGTGTAGTCAAATATACAGCATTCCTCTACAATGACCAACTTATCTGGAGTGGACTAGAGCAAGATGACATGAGAATTTTGTACAAATACCTCACCACCTCACTGTTCCCCAGGCACATCGAACCTGAGTTGGCGGGAAGGGACTCCCCAGTCAGGGCAGAGATGCCTGGGAATCTTCAGCACTATGGAAGATTTCTTACTGGACCCTTGAACCTTAATGATCCAGAAGCAAAATGCAGATTCCCCAAAATTTTTGTAAATACAGATGACACGTATGAAGAGCTCCATTTAATTGTTTATAAGGCCATGAGTGCAGCCGTGTGCTTCATGATTGACGCCTCCACGCCGCTCACGCTGGATTTCTGCCGAAGACTGGACAGCATCGTTGGGCCCCAGCTTACGGTGCTGGCATCTGACATCTGTGAGCAGTTTAATATCAACAAGAGGATCTCTGGGTCTGAAAAGGAGCCCCAGTTTAAGTTCATCTACTTCAACCACATGAATTTAGCAGAGAAAAGTACAATTCACATGAGGAAAACGCCCAGTGTGTCACTCACATCCGTCCACCCAGATCTAATGAAGATATTAGGGGACATCAACAGTGACTTTACCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAAAACGCCCAGTGTGTCAC
59.97
CCAACAGCTCCTCCTCTTGG
60.04
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAAACGCCCAGTGTGTCAC
59.97
CACCTTGTCCAACAGCTCCT
59.89
168
PCR template sequence:
AAAGTACAATTCACATGAGGAAAACGCCCAGTGTGTCACTCACATCCGTCCACCCAGATCTAATGAAGATATTAGGGGACATCAACAGTGACTTTACCAGGTTGTTCGGAATCCTATCATCGAGAAGCAAAGTAAAGATGGCAAGGCGGTAGTGGAGTACCAAGAGGAGGAGCTGTTGGACAAGGTGTACAGCTCAGTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-26a-5p(1), mmu-miR-26b-5p(1), mmu-miR-224-5p(1), mmu-miR-680(1), mmu-miR-539-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTGACTTTACCAGGTTGTTCGGAATC
siRNA sets
ATTCCGAACAACCTGGTAA, AACAACCTGGTAAAGTCAC, GAACAACCTGGTAAAGTCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCCZ1_953_ORF_1
0.9602
0.150069074
0.971241358
888
ORF sequence:
ATGTACAAGCTTTTCAATGGGACGTTTCTGAAAGCCATGGAAGATGGAGGCGTCAAGCTCCTAAAGGAAAGATTAGAGAAATTCTTCCATCGGTATTTGCAGACACTACATTTGCAGTCATGTGACCTACTTGATATTTTTGGTGGAATCAGCTTCTTCCCATTGGATAAAATGACTTACTTGAAAATCCAGTCCTTTATTAACCGAATGGAAGAAAGCCTAAGTGTAGTCAAATATACAGCATTCCTCTACAATGACCAACTTATCTGGAGTGGACTAGAGCAAGATGACATGAGAATTTTGTACAAATACCTCACCACCTCACTGTTCCCCAGGCACATCGAACCTGAGTTGGCGGGAAGGGACTCCCCAGTCAGGGCAGAGATGCCTGGGAATCTTCAGCACTATGGAAGATTTCTTACTGGACCCTTGAACCTTAATGATCCAGAAGCAAAATGCAGATTCCCCAAAATTTTTGTAAATACAGATGACACGTATGAAGAGCTCCATTTAATTGTTTATAAGGCCATGAGTGCAGCCGTGTGCTTCATGATTGACGCCTCCACGCCGCTCACGCTGGATTTCTGCCGAAGACTGGACAGCATCGTTGGGCCCCAGCTTACGGTGCTGGCATCTGACATCTGTGAGCAGTTTAATATCAACAAGAGGATCTCTGGGTCTGAAAAGGAGCCCCAGTTTAAGTTCATCTACTTCAACCACATGAATTTAGCAGAGAAAAGTACAATTCACATGAGGAAAACGCCCAGTGTGTCACTCACATCCGTCCACCCAGATCTAATGAAGATATTAGGGGACATCAACAGTGACTTTACCAGGTTGTTCGGAATCCTATCATCGAGAAGCAAAGTAAAGATGGCAAGGCGGTAG
Peptide sequence:
MYKLFNGTFLKAMEDGGVKLLKERLEKFFHRYLQTLHLQSCDLLDIFGGISFFPLDKMTYLKIQSFINRMEESLSVVKYTAFLYNDQLIWSGLEQDDMRILYKYLTTSLFPRHIEPELAGRDSPVRAEMPGNLQHYGRFLTGPLNLNDPEAKCRFPKIFVNTDDTYEELHLIVYKAMSAAVCFMIDASTPLTLDFCRRLDSIVGPQLTVLASDICEQFNINKRISGSEKEPQFKFIYFNHMNLAEKSTIHMRKTPSVSLTSVHPDLMKILGDINSDFTRLFGILSSRSKVKMARR
IRESfinder result
IRESfinder score
0.593760486
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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