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mmu_circCct6b_909
Basic information of circular RNA
PanCircBase ID:
mmu_circCct6b_909
mm39_Chr. Position ID:
chr11|82610760|82632946|-
Gene name:
Cct6b
Transcript:
ENSMUST00000021040.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
13,12,11,10,9,8,7,6
Exon/intron offsets:
0,2446,3879,16379,17156,19688,21341,22075
Genomic length:
22186
Exon/intron sizes in nt:
73,10,31,34,14,89,78,30,00,000
Flanking exon/introns:
chr11:82610260-82610760|chr11:82632946-82644459
Splice length:
909
Other database IDs:
CIRCpedia: MMU_CIRCpedia_229835, circAtlas: mmu-Cct6b_0002
circBase: hsa_circ_0043081, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_120180
Human Ortholog IDs:
Mature spliced Sequence:
GTTGATCCGAGGATTGGTTTTGGACCATGGTGCCCGTCATCCAAGGATGAGGAAGCAGGTGCGAGATGCATACATCCTCACTTGCAATGTCTCACTAGAGTATGAAAAAACAGAAGTGAGCTCTGGTTTCTTTTATAAGACTGTAGAAGAGAAAGAGAAATTGGTAAAAGCTGAAAGAAAATTTATTGAAGATAGAGTGCAGAAAATAATAGACCTAAAGCAGAAAGTCTGTGCTGAGTCCAATAAAGGATTTGTTGTCATTAATCAAAAGGGCATCGATCCGGTTTCCTTAGAAATGCTTGCAAAACATAACATCGTAGCTCTTCGCAGGGCAAAAAGAAGGAATCTTGAAAGGCTCACCCTTGCTTGTGGAGGATTGGCTGTCAACTCCTTTGAAGGCCTGAGTGAAGAGTGCCTGGGGCACGCTGGCCTTGTCTTCGAGTACGCATTGGGTGAAGAAAAGTTCACTTTTATTGAGGACTGTGTAAATCCGCTCTCTGTCACCTTGTTGGTCAAAGGGCCAAATAAGCATACTCTCATACAGATCAAGGACGCCTTAAGAGATGGACTCCGTGCGGTCAAAAATGCTATTGAAGACGGCTGTGTGGTTCCAGGGGCTGGGGCAGTGGAAGTTGCGATAGCAGAAGCTCTTGTTAACTACAAGCACCGTGTGCAGGGAAGAGTTCGCCTGGGGATCCAAGCCTTTGCTGATGCCTTGCTCATCATCCCGAAGGTTCTTGCTCAGAATTCTGGTTATGACCTGCAGGAAACACTGATAAAAATTCAAACTAAACATGCCGAATCAAAAGAACTCCTGGGCATAGACCTGAACACAGGGGAGCCGATGGCAGCAGCAGAAGCAGGAATCTGGGATAATTACTGTGTGAAAAAACATCTTCTTCACTCTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTCCTGGGCATAGACCTGA
59.66
GCACCTGCTTCCTCATCCTT
60.03
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTCCTGGGCATAGACCTGA
59.66
CCTCATCCTTGGATGACGGG
59.89
151
PCR template sequence:
AACTCCTGGGCATAGACCTGAACACAGGGGAGCCGATGGCAGCAGCAGAAGCAGGAATCTGGGATAATTACTGTGTGAAAAAACATCTTCTTCACTCTTGGTTGATCCGAGGATTGGTTTTGGACCATGGTGCCCGTCATCCAAGGATGAGGAAGCAGGTGCGAGATGCATACATCCTCACTTGCAATGTCTCACTAGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-362-5p(1), mmu-miR-324-3p(1), mmu-miR-335-3p(1), mmu-miR-3065-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTCTTCACTCTTGGTTGATCCGAGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCCT6B_909_ORF_1
1.0437
0.138520969
0.999954355
1770
ORF sequence:
ATGAGGAAGCAGGTGCGAGATGCATACATCCTCACTTGCAATGTCTCACTAGAGTATGAAAAAACAGAAGTGAGCTCTGGTTTCTTTTATAAGACTGTAGAAGAGAAAGAGAAATTGGTAAAAGCTGAAAGAAAATTTATTGAAGATAGAGTGCAGAAAATAATAGACCTAAAGCAGAAAGTCTGTGCTGAGTCCAATAAAGGATTTGTTGTCATTAATCAAAAGGGCATCGATCCGGTTTCCTTAGAAATGCTTGCAAAACATAACATCGTAGCTCTTCGCAGGGCAAAAAGAAGGAATCTTGAAAGGCTCACCCTTGCTTGTGGAGGATTGGCTGTCAACTCCTTTGAAGGCCTGAGTGAAGAGTGCCTGGGGCACGCTGGCCTTGTCTTCGAGTACGCATTGGGTGAAGAAAAGTTCACTTTTATTGAGGACTGTGTAAATCCGCTCTCTGTCACCTTGTTGGTCAAAGGGCCAAATAAGCATACTCTCATACAGATCAAGGACGCCTTAAGAGATGGACTCCGTGCGGTCAAAAATGCTATTGAAGACGGCTGTGTGGTTCCAGGGGCTGGGGCAGTGGAAGTTGCGATAGCAGAAGCTCTTGTTAACTACAAGCACCGTGTGCAGGGAAGAGTTCGCCTGGGGATCCAAGCCTTTGCTGATGCCTTGCTCATCATCCCGAAGGTTCTTGCTCAGAATTCTGGTTATGACCTGCAGGAAACACTGATAAAAATTCAAACTAAACATGCCGAATCAAAAGAACTCCTGGGCATAGACCTGAACACAGGGGAGCCGATGGCAGCAGCAGAAGCAGGAATCTGGGATAATTACTGTGTGAAAAAACATCTTCTTCACTCTTGGTTGATCCGAGGATTGGTTTTGGACCATGGTGCCCGTCATCCAAGGATGAGGAAGCAGGTGCGAGATGCATACATCCTCACTTGCAATGTCTCACTAGAGTATGAAAAAACAGAAGTGAGCTCTGGTTTCTTTTATAAGACTGTAGAAGAGAAAGAGAAATTGGTAAAAGCTGAAAGAAAATTTATTGAAGATAGAGTGCAGAAAATAATAGACCTAAAGCAGAAAGTCTGTGCTGAGTCCAATAAAGGATTTGTTGTCATTAATCAAAAGGGCATCGATCCGGTTTCCTTAGAAATGCTTGCAAAACATAACATCGTAGCTCTTCGCAGGGCAAAAAGAAGGAATCTTGAAAGGCTCACCCTTGCTTGTGGAGGATTGGCTGTCAACTCCTTTGAAGGCCTGAGTGAAGAGTGCCTGGGGCACGCTGGCCTTGTCTTCGAGTACGCATTGGGTGAAGAAAAGTTCACTTTTATTGAGGACTGTGTAAATCCGCTCTCTGTCACCTTGTTGGTCAAAGGGCCAAATAAGCATACTCTCATACAGATCAAGGACGCCTTAAGAGATGGACTCCGTGCGGTCAAAAATGCTATTGAAGACGGCTGTGTGGTTCCAGGGGCTGGGGCAGTGGAAGTTGCGATAGCAGAAGCTCTTGTTAACTACAAGCACCGTGTGCAGGGAAGAGTTCGCCTGGGGATCCAAGCCTTTGCTGATGCCTTGCTCATCATCCCGAAGGTTCTTGCTCAGAATTCTGGTTATGACCTGCAGGAAACACTGATAAAAATTCAAACTAAACATGCCGAATCAAAAGAACTCCTGGGCATAGACCTGAACACAGGGGAGCCGATGGCAGCAGCAGAAGCAGGAATCTGGGATAATTACTGTGTGAAAAAACATCTTCTTCACTCT
Peptide sequence:
MRKQVRDAYILTCNVSLEYEKTEVSSGFFYKTVEEKEKLVKAERKFIEDRVQKIIDLKQKVCAESNKGFVVINQKGIDPVSLEMLAKHNIVALRRAKRRNLERLTLACGGLAVNSFEGLSEECLGHAGLVFEYALGEEKFTFIEDCVNPLSVTLLVKGPNKHTLIQIKDALRDGLRAVKNAIEDGCVVPGAGAVEVAIAEALVNYKHRVQGRVRLGIQAFADALLIIPKVLAQNSGYDLQETLIKIQTKHAESKELLGIDLNTGEPMAAAEAGIWDNYCVKKHLLHSWLIRGLVLDHGARHPRMRKQVRDAYILTCNVSLEYEKTEVSSGFFYKTVEEKEKLVKAERKFIEDRVQKIIDLKQKVCAESNKGFVVINQKGIDPVSLEMLAKHNIVALRRAKRRNLERLTLACGGLAVNSFEGLSEECLGHAGLVFEYALGEEKFTFIEDCVNPLSVTLLVKGPNKHTLIQIKDALRDGLRAVKNAIEDGCVVPGAGAVEVAIAEALVNYKHRVQGRVRLGIQAFADALLIIPKVLAQNSGYDLQETLIKIQTKHAESKELLGIDLNTGEPMAAAEAGIWDNYCVKKHLLHS
IRESfinder result
IRESfinder score
0.564310681
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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