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mmu_circCcdc191_690
Basic information of circular RNA
PanCircBase ID:
mmu_circCcdc191_690
mm39_Chr. Position ID:
chr16|43725713|43735966|+
Gene name:
Ccdc191
Transcript:
ENSMUST00000132859.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
4,5,6,7
Exon/intron offsets:
0,1437,3061,10099
Genomic length:
10253
Exon/intron sizes in nt:
1,33,17,92,24,154
Flanking exon/introns:
chr16:43718594-43725713|chr16:43735966-43751556
Splice length:
690
Other database IDs:
CIRCpedia: MMU_CIRCpedia_20171, circAtlas: mmu-2610015P09Rik_0010
Human Ortholog IDs:
Mature spliced Sequence:
GAACTAGTCAATGACTGGCTGGACAACAAACTTAAACAAGAATTAGCAAGTGATGGAGAAGGGGAAAGCACTGTGCTGAGCAGCCCCCCTGTGCCAGACGCCCATGACCATTTGAAGTATGACAAGTTTGATGATTTATGTGGCTATTTGGAGGAAGAGGAGGAAAGTGCCACTGTTCAGAAATTTATAGAGCATCTGCTCCATAAAGAAGTGGTGGCTTCTGAGATGGTGGAAGAGCTTGGAATGGAGGAGAACCAAGACAAGAGGCAGCAGAAGGATCCTCGTCTTACCATGGAGATGAGACATAAGCAGGTCAAGGAAAATCGCTTAAGACGCGAGAAACAACTGGAGTCCCAGAGAATGGCAGATGCCCTGAAAAAAGCAGCCTTCTTGGAGGCTCAGAGTCTAGTGCAGGAAGAAAAGAGAAGGAAGGCTCTGCTGGCCAAGAAAGAGAAGGAGGACATTCAGAGAGAGATGGTAAAGCTGCGGAGGGAGATACTTGAAAGAAGGCACACTGTGGAAGAAGCATGGAAAACAGAAAAGAAAAGACAAGAAGAAATTTCTCAGAAGAATTCAGAGGGAAAAATGATGGAAAGTACTCACATACATCTGGATGAAGAAAAAGTTGCAAAAGAAAGAAAAAGAAAACTGAGGGAGACACTAATCCAGACTTTCAAGGAAAATCAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACATCTGGATGAAGAAAAAGTTGCA
59.7
CAGTGCTTTCCCCTTCTCCA
59.6
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACATCTGGATGAAGAAAAAGTTGCA
59.7
ACAGTGCTTTCCCCTTCTCC
59.6
159
PCR template sequence:
GGAAAGTACTCACATACATCTGGATGAAGAAAAAGTTGCAAAAGAAAGAAAAAGAAAACTGAGGGAGACACTAATCCAGACTTTCAAGGAAAATCAGCAGGAACTAGTCAATGACTGGCTGGACAACAAACTTAAACAAGAATTAGCAAGTGATGGAGAAGGGGAAAGCACTGTGCTGAGCAGCCCCCCTGTGCCAGACG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(1), mmu-miR-297a-5p(1), mmu-miR-680(1), mmu-miR-539-5p(1), mmu-miR-335-3p(1), mmu-miR-1904(1), mmu-miR-3105-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGAAAATCAGCAGGAACTAGTCAATG
siRNA sets
ATTGACTAGTTCCTGCTGA, TTGACTAGTTCCTGCTGAT, CTAGTTCCTGCTGATTTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCCDC191_690_ORF_1
1.1209
0.288383241
0.998208743
1155
ORF sequence:
ATGGTGGAAGAGCTTGGAATGGAGGAGAACCAAGACAAGAGGCAGCAGAAGGATCCTCGTCTTACCATGGAGATGAGACATAAGCAGGTCAAGGAAAATCGCTTAAGACGCGAGAAACAACTGGAGTCCCAGAGAATGGCAGATGCCCTGAAAAAAGCAGCCTTCTTGGAGGCTCAGAGTCTAGTGCAGGAAGAAAAGAGAAGGAAGGCTCTGCTGGCCAAGAAAGAGAAGGAGGACATTCAGAGAGAGATGGTAAAGCTGCGGAGGGAGATACTTGAAAGAAGGCACACTGTGGAAGAAGCATGGAAAACAGAAAAGAAAAGACAAGAAGAAATTTCTCAGAAGAATTCAGAGGGAAAAATGATGGAAAGTACTCACATACATCTGGATGAAGAAAAAGTTGCAAAAGAAAGAAAAAGAAAACTGAGGGAGACACTAATCCAGACTTTCAAGGAAAATCAGCAGGAACTAGTCAATGACTGGCTGGACAACAAACTTAAACAAGAATTAGCAAGTGATGGAGAAGGGGAAAGCACTGTGCTGAGCAGCCCCCCTGTGCCAGACGCCCATGACCATTTGAAGTATGACAAGTTTGATGATTTATGTGGCTATTTGGAGGAAGAGGAGGAAAGTGCCACTGTTCAGAAATTTATAGAGCATCTGCTCCATAAAGAAGTGGTGGCTTCTGAGATGGTGGAAGAGCTTGGAATGGAGGAGAACCAAGACAAGAGGCAGCAGAAGGATCCTCGTCTTACCATGGAGATGAGACATAAGCAGGTCAAGGAAAATCGCTTAAGACGCGAGAAACAACTGGAGTCCCAGAGAATGGCAGATGCCCTGAAAAAAGCAGCCTTCTTGGAGGCTCAGAGTCTAGTGCAGGAAGAAAAGAGAAGGAAGGCTCTGCTGGCCAAGAAAGAGAAGGAGGACATTCAGAGAGAGATGGTAAAGCTGCGGAGGGAGATACTTGAAAGAAGGCACACTGTGGAAGAAGCATGGAAAACAGAAAAGAAAAGACAAGAAGAAATTTCTCAGAAGAATTCAGAGGGAAAAATGATGGAAAGTACTCACATACATCTGGATGAAGAAAAAGTTGCAAAAGAAAGAAAAAGAAAACTGAGGGAGACACTAATCCAGACTTTCAAGGAAAATCAGCAG
Peptide sequence:
MVEELGMEENQDKRQQKDPRLTMEMRHKQVKENRLRREKQLESQRMADALKKAAFLEAQSLVQEEKRRKALLAKKEKEDIQREMVKLRREILERRHTVEEAWKTEKKRQEEISQKNSEGKMMESTHIHLDEEKVAKERKRKLRETLIQTFKENQQELVNDWLDNKLKQELASDGEGESTVLSSPPVPDAHDHLKYDKFDDLCGYLEEEEESATVQKFIEHLLHKEVVASEMVEELGMEENQDKRQQKDPRLTMEMRHKQVKENRLRREKQLESQRMADALKKAAFLEAQSLVQEEKRRKALLAKKEKEDIQREMVKLRREILERRHTVEEAWKTEKKRQEEISQKNSEGKMMESTHIHLDEEKVAKERKRKLRETLIQTFKENQQ
IRESfinder result
IRESfinder score
0.319249831
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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