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mmu_circCcdc186_961
Basic information of circular RNA
PanCircBase ID:
mmu_circCcdc186_961
mm39_Chr. Position ID:
chr19|56783521|56790421|-
Gene name:
Ccdc186
Transcript:
ENSMUST00000118592.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
13,12,11,10,9,8,7
Exon/intron offsets:
0,2354,3477,4974,5823,6077,6795
Genomic length:
6900
Exon/intron sizes in nt:
8,11,90,25,61,43,87,99,000
Flanking exon/introns:
chr19:56781938-56783521|chr19:56790421-56795397
Splice length:
961
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAAACCAAAGATAAACTCAAAGAAACAACTACAAAACTAACCCAGGCAAAGGAGGAAGCTGAGCAAATCCGGCAGAACTGTCAGGACATGATAAAGACCTATCAGGAATCTGAAGAAATTAAGTCAAATGAGCTTGATGCAAAGCTCAGAGTTACAAAAGGAGAACTTGAAAAACAAATGCAAGAGAAATCTGACCAGCTGGAGATGCATCATGCCAAAATAAAGGAGCTAGAAGATCTAAAGAGGACATTTAAGGAGGGCATGGATGAGCTACGGACATTGAGAACGAAGGCGAAATGTCTAGAAGATGAACGCTTAAGAACGGAAGATGAGTTATCAAAGTACAGGGAAATTATCAATCGACAGAAATCTGAAATTCAGAATTTACTGGACAAGGTGAAAATCACAGATCAGTTACATGAGCAGCTTCAAAGTGGTAAACAAGAAATTGAACATTTGAAAGAAGAAATGGAAAGCCTTAATTCTTTGATCAATGACCTCCAGAAAGACATTGAAGGCAGCAGGAAAAGGGAGTCTGAGCTCCTGCTTTTCACAGAAAAGCTCACGAGTAAGAACGCGCAGCTGCAGTCTGAGTCCAGCGCTCTGCAGTCCCAGGTGGACAACCTGTCCTGTACTGAGAGCCAGCTGCAAAGCCAGTGCCAGCAGATGGGACAGGCTAACCGTAATCTGGAGAGCAAGTTGCTCAAAGAGGAAGAGCTTCGAAAAGAGGAAGTACAGACTCTGCAAGCTGAGCTTTCTGCTGCGCAGACCGAAGTCAAAGCCTTGAGTACCCAGGTGGAAGAGCTGAAAGATGAGTTAGTGACGCAGAGGCGCAAACACGCCTCTAACGTCAAGGACCTCAGCAAGCAGCTTCAGCAAGCTCGGAGAAAGTTAGAGCAGACTGAGAATGGAAACCACGATAAAGACATTAGCAGTATGGGAAGTCGCTCGAGTTCATCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGCAAGCTCGGAGAAAGT
59.32
CCTGACAGTTCTGCCGGATT
60.04
174
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGCAAGCTCGGAGAAAGT
59.32
AGTTCTGCCGGATTTGCTCA
59.96
168
PCR template sequence:
AGCAAGCAGCTTCAGCAAGCTCGGAGAAAGTTAGAGCAGACTGAGAATGGAAACCACGATAAAGACATTAGCAGTATGGGAAGTCGCTCGAGTTCATCAGGAAACCAAAGATAAACTCAAAGAAACAACTACAAAACTAACCCAGGCAAAGGAGGAAGCTGAGCAAATCCGGCAGAACTGTCAGGACATGATAAAGACCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(1), mmu-miR-204-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCGAGTTCATCAGGAAACCAAAGATA
siRNA sets
TATCTTTGGTTTCCTGATG, ATCTTTGGTTTCCTGATGA, CTTTGGTTTCCTGATGAAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCCDC186_961_ORF_1
1.2035
0.319208464
0.996213508
1014
ORF sequence:
ATGGAAACCACGATAAAGACATTAGCAGTATGGGAAGTCGCTCGAGTTCATCAGGAAACCAAAGATAAACTCAAAGAAACAACTACAAAACTAACCCAGGCAAAGGAGGAAGCTGAGCAAATCCGGCAGAACTGTCAGGACATGATAAAGACCTATCAGGAATCTGAAGAAATTAAGTCAAATGAGCTTGATGCAAAGCTCAGAGTTACAAAAGGAGAACTTGAAAAACAAATGCAAGAGAAATCTGACCAGCTGGAGATGCATCATGCCAAAATAAAGGAGCTAGAAGATCTAAAGAGGACATTTAAGGAGGGCATGGATGAGCTACGGACATTGAGAACGAAGGCGAAATGTCTAGAAGATGAACGCTTAAGAACGGAAGATGAGTTATCAAAGTACAGGGAAATTATCAATCGACAGAAATCTGAAATTCAGAATTTACTGGACAAGGTGAAAATCACAGATCAGTTACATGAGCAGCTTCAAAGTGGTAAACAAGAAATTGAACATTTGAAAGAAGAAATGGAAAGCCTTAATTCTTTGATCAATGACCTCCAGAAAGACATTGAAGGCAGCAGGAAAAGGGAGTCTGAGCTCCTGCTTTTCACAGAAAAGCTCACGAGTAAGAACGCGCAGCTGCAGTCTGAGTCCAGCGCTCTGCAGTCCCAGGTGGACAACCTGTCCTGTACTGAGAGCCAGCTGCAAAGCCAGTGCCAGCAGATGGGACAGGCTAACCGTAATCTGGAGAGCAAGTTGCTCAAAGAGGAAGAGCTTCGAAAAGAGGAAGTACAGACTCTGCAAGCTGAGCTTTCTGCTGCGCAGACCGAAGTCAAAGCCTTGAGTACCCAGGTGGAAGAGCTGAAAGATGAGTTAGTGACGCAGAGGCGCAAACACGCCTCTAACGTCAAGGACCTCAGCAAGCAGCTTCAGCAAGCTCGGAGAAAGTTAGAGCAGACTGAGAATGGAAACCACGATAAAGACATTAGCAGTATGGGAAGTCGCTCGAGTTCATCA
Peptide sequence:
METTIKTLAVWEVARVHQETKDKLKETTTKLTQAKEEAEQIRQNCQDMIKTYQESEEIKSNELDAKLRVTKGELEKQMQEKSDQLEMHHAKIKELEDLKRTFKEGMDELRTLRTKAKCLEDERLRTEDELSKYREIINRQKSEIQNLLDKVKITDQLHEQLQSGKQEIEHLKEEMESLNSLINDLQKDIEGSRKRESELLLFTEKLTSKNAQLQSESSALQSQVDNLSCTESQLQSQCQQMGQANRNLESKLLKEEELRKEEVQTLQAELSAAQTEVKALSTQVEELKDELVTQRRKHASNVKDLSKQLQQARRKLEQTENGNHDKDISSMGSRSSSS
IRESfinder result
IRESfinder score
0.342630928
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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