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mmu_circCcdc186_880_2
Basic information of circular RNA
PanCircBase ID:
mmu_circCcdc186_880_2
mm39_Chr. Position ID:
chr19|56789344|56798797|-
Gene name:
Ccdc186
Transcript:
ENSMUST00000118592.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
9,8,7,6,5,4,3
Exon/intron offsets:
0,254,972,6053,7065,8280,9326
Genomic length:
9453
Exon/intron sizes in nt:
87,99,10,51,20,21,31,20,000
Flanking exon/introns:
chr19:56788638-56789344|chr19:56798797-56801426
Splice length:
880
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTAATTAAAGAAAATAAGAATCATCAGGAACTTATCTTAAACATTTGTTCAGAAAAAGACAGTTTAAGAGAAGAACTGAGAAAAAGGACAGAAACAGAGAAGCAGCATATGAACACAATAAAGCAGTTAGAATTAAGAATAGAGGAACTGAATAAAGAAATTAAAGCTTCCAAAGATCAGCTAGTCGCACAGGATGTCACAGCTAAAAATGCAATTCAGCAAATCCATAAAGAGATGGCCCAGCGGATGGACCAGGCCAATAAGAAATGTGAAGAGGCCCGTCAGGAAAAGGAGGCAATGGTAATGAAGTATGTGAGAGGCGAGAAGGAGGCTCTAGACCTGCGAAAGGAAAAAGAAACCCTTGAGAGGAAACTTAGAGATGCAAGTAAGGAATTGGAGAAAAACACTAACAAAATTAAGCAGCTGTCTCAGGAGAAAGGGCGGCTGCAGCAACTCTATGAATCGAAGGAAGGTGAAACAACTAGACTCATCAGAGAAATAGAGAAATTAAAGGAGGAGATGAATTCTCAAGTCATTAAAGTAAAGTGGGCGCAAAACAAGTTAAAAGCTGAAATGGACTCACACAAGGAAACCAAAGATAAACTCAAAGAAACAACTACAAAACTAACCCAGGCAAAGGAGGAAGCTGAGCAAATCCGGCAGAACTGTCAGGACATGATAAAGACCTATCAGGAATCTGAAGAAATTAAGTCAAATGAGCTTGATGCAAAGCTCAGAGTTACAAAAGGAGAACTTGAAAAACAAATGCAAGAGAAATCTGACCAGCTGGAGATGCATCATGCCAAAATAAAGGAGCTAGAAGATCTAAAGAGGACATTTAAGGAGGGCATGGATGAGCTACGGACATTGAGAACGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TAAGGAGGGCATGGATGAGC
59.23
CTCTGTTTCTGTCCTTTTTCTCAGT
59.18
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTAAGGAGGGCATGGATGAGC
59.86
TCTGTTTCTGTCCTTTTTCTCAGT
58.25
140
PCR template sequence:
TGACCAGCTGGAGATGCATCATGCCAAAATAAAGGAGCTAGAAGATCTAAAGAGGACATTTAAGGAGGGCATGGATGAGCTACGGACATTGAGAACGAAGGTTAATTAAAGAAAATAAGAATCATCAGGAACTTATCTTAAACATTTGTTCAGAAAAAGACAGTTTAAGAGAAGAACTGAGAAAAAGGACAGAAACAGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATTGAGAACGAAGGTTAATTAAAGAA
siRNA sets
TAATTAACCTTCGTTCTCA, TTCTTTAATTAACCTTCGT, TTTAATTAACCTTCGTTCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCCDC186_880_2_ORF_1
1.1532
0.245279932
0.970455939
780
ORF sequence:
ATGAACACAATAAAGCAGTTAGAATTAAGAATAGAGGAACTGAATAAAGAAATTAAAGCTTCCAAAGATCAGCTAGTCGCACAGGATGTCACAGCTAAAAATGCAATTCAGCAAATCCATAAAGAGATGGCCCAGCGGATGGACCAGGCCAATAAGAAATGTGAAGAGGCCCGTCAGGAAAAGGAGGCAATGGTAATGAAGTATGTGAGAGGCGAGAAGGAGGCTCTAGACCTGCGAAAGGAAAAAGAAACCCTTGAGAGGAAACTTAGAGATGCAAGTAAGGAATTGGAGAAAAACACTAACAAAATTAAGCAGCTGTCTCAGGAGAAAGGGCGGCTGCAGCAACTCTATGAATCGAAGGAAGGTGAAACAACTAGACTCATCAGAGAAATAGAGAAATTAAAGGAGGAGATGAATTCTCAAGTCATTAAAGTAAAGTGGGCGCAAAACAAGTTAAAAGCTGAAATGGACTCACACAAGGAAACCAAAGATAAACTCAAAGAAACAACTACAAAACTAACCCAGGCAAAGGAGGAAGCTGAGCAAATCCGGCAGAACTGTCAGGACATGATAAAGACCTATCAGGAATCTGAAGAAATTAAGTCAAATGAGCTTGATGCAAAGCTCAGAGTTACAAAAGGAGAACTTGAAAAACAAATGCAAGAGAAATCTGACCAGCTGGAGATGCATCATGCCAAAATAAAGGAGCTAGAAGATCTAAAGAGGACATTTAAGGAGGGCATGGATGAGCTACGGACATTGAGAACGAAGGTTAATTAA
Peptide sequence:
MNTIKQLELRIEELNKEIKASKDQLVAQDVTAKNAIQQIHKEMAQRMDQANKKCEEARQEKEAMVMKYVRGEKEALDLRKEKETLERKLRDASKELEKNTNKIKQLSQEKGRLQQLYESKEGETTRLIREIEKLKEEMNSQVIKVKWAQNKLKAEMDSHKETKDKLKETTTKLTQAKEEAEQIRQNCQDMIKTYQESEEIKSNELDAKLRVTKGELEKQMQEKSDQLEMHHAKIKELEDLKRTFKEGMDELRTLRTKVN
IRESfinder result
IRESfinder score
0.356904295
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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