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mmu_circCcdc186_1213
Basic information of circular RNA
PanCircBase ID:
mmu_circCcdc186_1213
mm39_Chr. Position ID:
chr19|56785875|56796622|-
Gene name:
Ccdc186
Transcript:
ENSMUST00000118592.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
12,11,10,9,8,7,6,5
Exon/intron offsets:
0,1123,2620,3469,3723,4441,9522,10534
Genomic length:
10747
Exon/intron sizes in nt:
1,90,25,61,43,87,99,10,00,00,000
Flanking exon/introns:
chr19:56783602-56785875|chr19:56796622-56797624
Splice length:
1213
Other database IDs:
circAtlas: mmu-A630007B06Rik_0017
Human Ortholog IDs:
Mature spliced Sequence:
GCCAATAAGAAATGTGAAGAGGCCCGTCAGGAAAAGGAGGCAATGGTAATGAAGTATGTGAGAGGCGAGAAGGAGGCTCTAGACCTGCGAAAGGAAAAAGAAACCCTTGAGAGGAAACTTAGAGATGCAAGTAAGGAATTGGAGAAAAACACTAACAAAATTAAGCAGCTGTCTCAGGAGAAAGGGCGGCTGCAGCAACTCTATGAATCGAAGGAAGGTGAAACAACTAGACTCATCAGAGAAATAGAGAAATTAAAGGAGGAGATGAATTCTCAAGTCATTAAAGTAAAGTGGGCGCAAAACAAGTTAAAAGCTGAAATGGACTCACACAAGGAAACCAAAGATAAACTCAAAGAAACAACTACAAAACTAACCCAGGCAAAGGAGGAAGCTGAGCAAATCCGGCAGAACTGTCAGGACATGATAAAGACCTATCAGGAATCTGAAGAAATTAAGTCAAATGAGCTTGATGCAAAGCTCAGAGTTACAAAAGGAGAACTTGAAAAACAAATGCAAGAGAAATCTGACCAGCTGGAGATGCATCATGCCAAAATAAAGGAGCTAGAAGATCTAAAGAGGACATTTAAGGAGGGCATGGATGAGCTACGGACATTGAGAACGAAGGCGAAATGTCTAGAAGATGAACGCTTAAGAACGGAAGATGAGTTATCAAAGTACAGGGAAATTATCAATCGACAGAAATCTGAAATTCAGAATTTACTGGACAAGGTGAAAATCACAGATCAGTTACATGAGCAGCTTCAAAGTGGTAAACAAGAAATTGAACATTTGAAAGAAGAAATGGAAAGCCTTAATTCTTTGATCAATGACCTCCAGAAAGACATTGAAGGCAGCAGGAAAAGGGAGTCTGAGCTCCTGCTTTTCACAGAAAAGCTCACGAGTAAGAACGCGCAGCTGCAGTCTGAGTCCAGCGCTCTGCAGTCCCAGGTGGACAACCTGTCCTGTACTGAGAGCCAGCTGCAAAGCCAGTGCCAGCAGATGGGACAGGCTAACCGTAATCTGGAGAGCAAGTTGCTCAAAGAGGAAGAGCTTCGAAAAGAGGAAGTACAGACTCTGCAAGCTGAGCTTTCTGCTGCGCAGACCGAAGTCAAAGCCTTGAGTACCCAGGTGGAAGAGCTGAAAGATGAGTTAGTGACGCAGAGGCGCAAACACGCCTCTAACGTCAAGGACCTCAGCAAGCAGCTTCAGCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAAACACGCCTCTAACGTC
59.84
CCTTTCGCAGGTCTAGAGCC
60.18
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGAGTTAGTGACGCAGAGGC
59.76
CCTTTCGCAGGTCTAGAGCC
60.18
162
PCR template sequence:
GCCTTGAGTACCCAGGTGGAAGAGCTGAAAGATGAGTTAGTGACGCAGAGGCGCAAACACGCCTCTAACGTCAAGGACCTCAGCAAGCAGCTTCAGCAAGGCCAATAAGAAATGTGAAGAGGCCCGTCAGGAAAAGGAGGCAATGGTAATGAAGTATGTGAGAGGCGAGAAGGAGGCTCTAGACCTGCGAAAGGAAAAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGCTTCAGCAAGGCCAATAAGAAAT
siRNA sets
ATTTCTTATTGGCCTTGCT, TTTCTTATTGGCCTTGCTG, TTATTGGCCTTGCTGAAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCCDC186_1213_ORF_1
1.2629
0.302190134
0.998878906
1179
ORF sequence:
ATGGTAATGAAGTATGTGAGAGGCGAGAAGGAGGCTCTAGACCTGCGAAAGGAAAAAGAAACCCTTGAGAGGAAACTTAGAGATGCAAGTAAGGAATTGGAGAAAAACACTAACAAAATTAAGCAGCTGTCTCAGGAGAAAGGGCGGCTGCAGCAACTCTATGAATCGAAGGAAGGTGAAACAACTAGACTCATCAGAGAAATAGAGAAATTAAAGGAGGAGATGAATTCTCAAGTCATTAAAGTAAAGTGGGCGCAAAACAAGTTAAAAGCTGAAATGGACTCACACAAGGAAACCAAAGATAAACTCAAAGAAACAACTACAAAACTAACCCAGGCAAAGGAGGAAGCTGAGCAAATCCGGCAGAACTGTCAGGACATGATAAAGACCTATCAGGAATCTGAAGAAATTAAGTCAAATGAGCTTGATGCAAAGCTCAGAGTTACAAAAGGAGAACTTGAAAAACAAATGCAAGAGAAATCTGACCAGCTGGAGATGCATCATGCCAAAATAAAGGAGCTAGAAGATCTAAAGAGGACATTTAAGGAGGGCATGGATGAGCTACGGACATTGAGAACGAAGGCGAAATGTCTAGAAGATGAACGCTTAAGAACGGAAGATGAGTTATCAAAGTACAGGGAAATTATCAATCGACAGAAATCTGAAATTCAGAATTTACTGGACAAGGTGAAAATCACAGATCAGTTACATGAGCAGCTTCAAAGTGGTAAACAAGAAATTGAACATTTGAAAGAAGAAATGGAAAGCCTTAATTCTTTGATCAATGACCTCCAGAAAGACATTGAAGGCAGCAGGAAAAGGGAGTCTGAGCTCCTGCTTTTCACAGAAAAGCTCACGAGTAAGAACGCGCAGCTGCAGTCTGAGTCCAGCGCTCTGCAGTCCCAGGTGGACAACCTGTCCTGTACTGAGAGCCAGCTGCAAAGCCAGTGCCAGCAGATGGGACAGGCTAACCGTAATCTGGAGAGCAAGTTGCTCAAAGAGGAAGAGCTTCGAAAAGAGGAAGTACAGACTCTGCAAGCTGAGCTTTCTGCTGCGCAGACCGAAGTCAAAGCCTTGAGTACCCAGGTGGAAGAGCTGAAAGATGAGTTAGTGACGCAGAGGCGCAAACACGCCTCTAACGTCAAGGACCTCAGCAAGCAGCTTCAGCAAGGCCAATAA
Peptide sequence:
MVMKYVRGEKEALDLRKEKETLERKLRDASKELEKNTNKIKQLSQEKGRLQQLYESKEGETTRLIREIEKLKEEMNSQVIKVKWAQNKLKAEMDSHKETKDKLKETTTKLTQAKEEAEQIRQNCQDMIKTYQESEEIKSNELDAKLRVTKGELEKQMQEKSDQLEMHHAKIKELEDLKRTFKEGMDELRTLRTKAKCLEDERLRTEDELSKYREIINRQKSEIQNLLDKVKITDQLHEQLQSGKQEIEHLKEEMESLNSLINDLQKDIEGSRKRESELLLFTEKLTSKNAQLQSESSALQSQVDNLSCTESQLQSQCQQMGQANRNLESKLLKEEELRKEEVQTLQAELSAAQTEVKALSTQVEELKDELVTQRRKHASNVKDLSKQLQQGQ
IRESfinder result
IRESfinder score
0.33025956
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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