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mmu_circCard11_464
Basic information of circular RNA
PanCircBase ID:
mmu_circCard11_464
mm39_Chr. Position ID:
chr5|140892379|140894407|-
Gene name:
Card11
Transcript:
ENSMUST00000085786.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
5,4
Exon/intron offsets:
0,1890
Genomic length:
2028
Exon/intron sizes in nt:
3,26,138
Flanking exon/introns:
chr5:140888672-140892379|chr5:140894407-140898952
Splice length:
464
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCCGATTGTTGGACATTCTTCACACCAAGGGACAAAGGGGCTATGTGGTCTTCCTGGAGAGCCTGGAGTTTTACTACCCAGAACTTTACAAACTGGTGACTGGAAAGGAACCCACCCGGAGATTCTCCACCATTGTGGTGGAGGAAGGCCATGAGGGCCTCACACACTTCCTGATGAACGAGGTCATCAAACTGCAGCAGCAAGTGAAAGCCAAGGACCTTCAGCGCTGTGAGCTGCTGGCCAAGTCCCGGCAACTGGAGGATGAGAAGAAGCAGCTGAGCCTGATACGGGTGGAGCTGCTGACCTTCCAGGAGCGATACTACAAGATGAAGGAGGAGCGGGACAGCTACAATGACGAGCTCGTCAAGGTCAAGGACGACAACTACAACTTAGCCATGCGCTACGCCCAGCTCAGTGAGGAGAAAAACATGGCGGTGATGAGGAGCCGCGACCTCCAACTCGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTACAACTTAGCCATGCGC
58.63
AACTCCAGGCTCTCCAGGAA
60.18
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTACAACTTAGCCATGCGC
58.63
AAACTCCAGGCTCTCCAGGA
60.18
154
PCR template sequence:
CAAGGTCAAGGACGACAACTACAACTTAGCCATGCGCTACGCCCAGCTCAGTGAGGAGAAAAACATGGCGGTGATGAGGAGCCGCGACCTCCAACTCGAGGCCGATTGTTGGACATTCTTCACACCAAGGGACAAAGGGGCTATGTGGTCTTCCTGGAGAGCCTGGAGTTTTACTACCCAGAACTTTACAAACTGGTGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-328-3p(1), mmu-miR-7a-5p(1), mmu-miR-673-5p(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
CCTCCAACTCGAGGCCGATTGTTGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCARD11_464_ORF_1
1.2859
0.560580473
0.903160878
390
ORF sequence:
ATGAACGAGGTCATCAAACTGCAGCAGCAAGTGAAAGCCAAGGACCTTCAGCGCTGTGAGCTGCTGGCCAAGTCCCGGCAACTGGAGGATGAGAAGAAGCAGCTGAGCCTGATACGGGTGGAGCTGCTGACCTTCCAGGAGCGATACTACAAGATGAAGGAGGAGCGGGACAGCTACAATGACGAGCTCGTCAAGGTCAAGGACGACAACTACAACTTAGCCATGCGCTACGCCCAGCTCAGTGAGGAGAAAAACATGGCGGTGATGAGGAGCCGCGACCTCCAACTCGAGGCCGATTGTTGGACATTCTTCACACCAAGGGACAAAGGGGCTATGTGGTCTTCCTGGAGAGCCTGGAGTTTTACTACCCAGAACTTTACAAACTGGTGA
Peptide sequence:
MNEVIKLQQQVKAKDLQRCELLAKSRQLEDEKKQLSLIRVELLTFQERYYKMKEERDSYNDELVKVKDDNYNLAMRYAQLSEEKNMAVMRSRDLQLEADCWTFFTPRDKGAMWSSWRAWSFTTQNFTNW
IRESfinder result
IRESfinder score
0.226149863
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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