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mmu_circCar10_330
Basic information of circular RNA
PanCircBase ID:
mmu_circCar10_330
mm39_Chr. Position ID:
chr11|93487938|93490224|+
Gene name:
Car10
Transcript:
ENSMUST00000042943.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
8,9
Exon/intron offsets:
0,2111
Genomic length:
2286
Exon/intron sizes in nt:
1,55,175
Flanking exon/introns:
chr11:93473846-93487938|chr11:93490224-93491141
Splice length:
330
Other database IDs:
circAtlas: mmu-Car10_0025
Human Ortholog IDs:
Mature spliced Sequence:
ATGATGCATACTTACTACAGGGACTTAATATAGAGGAACTGTATCCAGAGACCTCTAGTTTCATCACTTATGATGGGTCGATGACTATCCCGCCCTGCTATGAGACAGCAAGTTGGATAATAATGAACAAGCCTGTGTACATAACCAGGATGCAGATGCATTCCCTACGCCTCCTCAGCCAGAATCAGCCATCGCAGATCTTTCTGAGCATGAGTGACAACTTCAGGCCTGTCCAGCCACTCAATAATCGCTGTATTCGCACCAACATCAACTTCAGTTTACAGGGGAAGGACTGTCCTAACAACAGGGCCCAGAAGCTACAGTACAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCGCTGTATTCGCACCAACA
60.32
AGGGCGGGATAGTCATCGA
59.85
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCGCTGTATTCGCACCAACA
60.32
CAGGGCGGGATAGTCATCGA
61.18
180
PCR template sequence:
GTCCAGCCACTCAATAATCGCTGTATTCGCACCAACATCAACTTCAGTTTACAGGGGAAGGACTGTCCTAACAACAGGGCCCAGAAGCTACAGTACAGAGATGATGCATACTTACTACAGGGACTTAATATAGAGGAACTGTATCCAGAGACCTCTAGTTTCATCACTTATGATGGGTCGATGACTATCCCGCCCTGCTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-10b-5p(1), mmu-miR-10a-5p(1), mmu-miR-127-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTACAGTACAGAGATGATGCATACTT
siRNA sets
AAGTATGCATCATCTCTGT, ATGCATCATCTCTGTACTG, GTATGCATCATCTCTGTAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCAR10_330_ORF_1
1.1329
0.207501882
0.867023474
579
ORF sequence:
ATGACTATCCCGCCCTGCTATGAGACAGCAAGTTGGATAATAATGAACAAGCCTGTGTACATAACCAGGATGCAGATGCATTCCCTACGCCTCCTCAGCCAGAATCAGCCATCGCAGATCTTTCTGAGCATGAGTGACAACTTCAGGCCTGTCCAGCCACTCAATAATCGCTGTATTCGCACCAACATCAACTTCAGTTTACAGGGGAAGGACTGTCCTAACAACAGGGCCCAGAAGCTACAGTACAGAGATGATGCATACTTACTACAGGGACTTAATATAGAGGAACTGTATCCAGAGACCTCTAGTTTCATCACTTATGATGGGTCGATGACTATCCCGCCCTGCTATGAGACAGCAAGTTGGATAATAATGAACAAGCCTGTGTACATAACCAGGATGCAGATGCATTCCCTACGCCTCCTCAGCCAGAATCAGCCATCGCAGATCTTTCTGAGCATGAGTGACAACTTCAGGCCTGTCCAGCCACTCAATAATCGCTGTATTCGCACCAACATCAACTTCAGTTTACAGGGGAAGGACTGTCCTAACAACAGGGCCCAGAAGCTACAGTACAGA
Peptide sequence:
MTIPPCYETASWIIMNKPVYITRMQMHSLRLLSQNQPSQIFLSMSDNFRPVQPLNNRCIRTNINFSLQGKDCPNNRAQKLQYRDDAYLLQGLNIEELYPETSSFITYDGSMTIPPCYETASWIIMNKPVYITRMQMHSLRLLSQNQPSQIFLSMSDNFRPVQPLNNRCIRTNINFSLQGKDCPNNRAQKLQYR
IRESfinder result
IRESfinder score
0.515374187
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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