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mmu_circCapza1_323
Basic information of circular RNA
PanCircBase ID:
mmu_circCapza1_323
mm39_Chr. Position ID:
chr3|104747097|104750028|-
Gene name:
Capza1
Transcript:
ENSMUST00000094028.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,4,3
Exon/intron offsets:
0,1046,2879
Genomic length:
2931
Exon/intron sizes in nt:
207,64,52
Flanking exon/introns:
chr3:104742120-104747097|chr3:104750028-104752233
Splice length:
323
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGTCCGGCTACTGCTTAACAATGACAATCTCCTCAGGGAAGGGGCAGCACATGCATTTGCCCAGTATAACATGGATCAGTTCACACCCGTGAAGATAGAAGGCTACGATGACCAGGTCTTAATTACAGAGCATGGTGACCTTGGTAACAGCAGATTTTTAGATCCAAGAAACCAAATTTCCTTTAAGTTTGATCATTTACGGAAAGAAGCAAGTGACCCGCAGCCAGAGGATGTGGATGGAGGCTTGAAGTCTTGGAGGGAGTCGTGTGACAGTGCTCTGAGAGCCTATGTGAAAGATCATTATTCCAACGGCTTCTGTACT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAGGATGTGGATGGAGGCT
60.03
TACTGGGCAAATGCATGTGC
59.47
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTTGGAGGGAGTCGTGTG
60.04
TACTGGGCAAATGCATGTGC
59.47
140
PCR template sequence:
GCCAGAGGATGTGGATGGAGGCTTGAAGTCTTGGAGGGAGTCGTGTGACAGTGCTCTGAGAGCCTATGTGAAAGATCATTATTCCAACGGCTTCTGTACTATGTCCGGCTACTGCTTAACAATGACAATCTCCTCAGGGAAGGGGCAGCACATGCATTTGCCCAGTATAACATGGATCAGTTCACACCCGTGAAGATAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-351-5p(1), mmu-miR-670-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
CGGCTTCTGTACTATGTCCGGCTACT
siRNA sets
CGGACATAGTACAGAAGCC, GGACATAGTACAGAAGCCG, GTAGCCGGACATAGTACAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCAPZA1_323_ORF_1
0.6622
0.099949763
0.244437063
345
ORF sequence:
ATGGATCAGTTCACACCCGTGAAGATAGAAGGCTACGATGACCAGGTCTTAATTACAGAGCATGGTGACCTTGGTAACAGCAGATTTTTAGATCCAAGAAACCAAATTTCCTTTAAGTTTGATCATTTACGGAAAGAAGCAAGTGACCCGCAGCCAGAGGATGTGGATGGAGGCTTGAAGTCTTGGAGGGAGTCGTGTGACAGTGCTCTGAGAGCCTATGTGAAAGATCATTATTCCAACGGCTTCTGTACTATGTCCGGCTACTGCTTAACAATGACAATCTCCTCAGGGAAGGGGCAGCACATGCATTTGCCCAGTATAACATGGATCAGTTCACACCCGTGA
Peptide sequence:
MDQFTPVKIEGYDDQVLITEHGDLGNSRFLDPRNQISFKFDHLRKEASDPQPEDVDGGLKSWRESCDSALRAYVKDHYSNGFCTMSGYCLTMTISSGKGQHMHLPSITWISSHP
IRESfinder result
IRESfinder score
0.509375463
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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