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mmu_circCamsap2_673
Basic information of circular RNA
PanCircBase ID:
mmu_circCamsap2_673
mm39_Chr. Position ID:
chr1|136215637|136232194|-
Gene name:
Camsap2
Transcript:
ENSMUST00000048309.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
9,8,7,6,5,4,3
Exon/intron offsets:
0,4980,5380,6110,7614,9842,16395
Genomic length:
16557
Exon/intron sizes in nt:
94,14,01,42,37,14,84,100
Flanking exon/introns:
chr1:136213740-136215637|chr1:136232194-136252474
Splice length:
673
Other database IDs:
circAtlas: mmu-Camsap2_0011
Human Ortholog IDs:
Mature spliced Sequence:
AGTGCACATTTGGCTATGATTGATACTCTAATGATGGCTTACACTGTAGAGATGATCAGCATAGAAAAAGTAATTGCATGTGCTCAACAGTATTCAGCTTTTTTTCAAGCCACAGATCTGCCCTATGATATTGAGGATGCTGTCATGTACTGGATGAATAAGGTAAATGAACACTTGAAAGACATAATGGAACAAGAACAAAAATCTAAAGAGCACCACCCAGCTGAAGCTCCAGGAGGCCAAAAGGCAGGTCTGGAGCATACTTTTTGCCACTCACCAATTTTGATAGCTCTTATAGCTCGTTACAGGAAGGAACAAACACTGCTTAAGCAACTGCCTTGCATTCCATTGGTGGAAAACTTGTTGAAGGATGGGACCGACGGCTGCGCACTAGCTGCCCTCATTCACTTCTACTGCCCTGCTGTTGTCCGGCTAGAGGATATTTGTTTGAAAGAAACTATGTCTTTGGCTGACAGTCTGTACAATCTACAGCTGATTCAGGAATTTTGCCAAGAATACTTAAACCATTGCTGCCATTTCAGCTTGGAAGATATGCTTTATGCTGCTTCATCCATAAAGAGTAATTATTTGGTGTTCATGGCAGAACTCTTCTGGTGGTTTGAAGTTGTGAAGCCTTCATTTGTGCAGCCTCGTGTTGTCCGTCCACAAGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTTCATTTGTGCAGCCTC
60.11
AGCTGAATACTGTTGAGCACA
57.59
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGCCTTCATTTGTGCAGCC
59.68
AGCTGAATACTGTTGAGCACA
57.59
142
PCR template sequence:
ATAAAGAGTAATTATTTGGTGTTCATGGCAGAACTCTTCTGGTGGTTTGAAGTTGTGAAGCCTTCATTTGTGCAGCCTCGTGTTGTCCGTCCACAAGGAGAGTGCACATTTGGCTATGATTGATACTCTAATGATGGCTTACACTGTAGAGATGATCAGCATAGAAAAAGTAATTGCATGTGCTCAACAGTATTCAGCTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-15a-5p(1), mmu-miR-1a-3p(1), mmu-miR-429-3p(1), mmu-miR-206-3p(1), mmu-miR-200c-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-184-3p(1), mmu-miR-320-3p(1), mmu-miR-139-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CGTCCACAAGGAGAGTGCACATTTGG
siRNA sets
AATGTGCACTCTCCTTGTG, AAATGTGCACTCTCCTTGT, TGTGCACTCTCCTTGTGGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCAMSAP2_673_ORF_1
0.9174
0.073853467
0.838584776
681
ORF sequence:
ATGATTGATACTCTAATGATGGCTTACACTGTAGAGATGATCAGCATAGAAAAAGTAATTGCATGTGCTCAACAGTATTCAGCTTTTTTTCAAGCCACAGATCTGCCCTATGATATTGAGGATGCTGTCATGTACTGGATGAATAAGGTAAATGAACACTTGAAAGACATAATGGAACAAGAACAAAAATCTAAAGAGCACCACCCAGCTGAAGCTCCAGGAGGCCAAAAGGCAGGTCTGGAGCATACTTTTTGCCACTCACCAATTTTGATAGCTCTTATAGCTCGTTACAGGAAGGAACAAACACTGCTTAAGCAACTGCCTTGCATTCCATTGGTGGAAAACTTGTTGAAGGATGGGACCGACGGCTGCGCACTAGCTGCCCTCATTCACTTCTACTGCCCTGCTGTTGTCCGGCTAGAGGATATTTGTTTGAAAGAAACTATGTCTTTGGCTGACAGTCTGTACAATCTACAGCTGATTCAGGAATTTTGCCAAGAATACTTAAACCATTGCTGCCATTTCAGCTTGGAAGATATGCTTTATGCTGCTTCATCCATAAAGAGTAATTATTTGGTGTTCATGGCAGAACTCTTCTGGTGGTTTGAAGTTGTGAAGCCTTCATTTGTGCAGCCTCGTGTTGTCCGTCCACAAGGAGAGTGCACATTTGGCTATGATTGA
Peptide sequence:
MIDTLMMAYTVEMISIEKVIACAQQYSAFFQATDLPYDIEDAVMYWMNKVNEHLKDIMEQEQKSKEHHPAEAPGGQKAGLEHTFCHSPILIALIARYRKEQTLLKQLPCIPLVENLLKDGTDGCALAALIHFYCPAVVRLEDICLKETMSLADSLYNLQLIQEFCQEYLNHCCHFSLEDMLYAASSIKSNYLVFMAELFWWFEVVKPSFVQPRVVRPQGECTFGYD
IRESfinder result
IRESfinder score
0.665896455
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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