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mmu_circCadps_307
Basic information of circular RNA
PanCircBase ID:
mmu_circCadps_307
mm39_Chr. Position ID:
chr14|10019893|10029892|+
Gene name:
Cadps
Transcript:
ENSMUST00000112657.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
25,26,27
Exon/intron offsets:
0,8954,9915
Genomic length:
9999
Exon/intron sizes in nt:
148,75,84
Flanking exon/introns:
chr14:10015599-10019893|chr14:10029892-10051725
Splice length:
307
Other database IDs:
circBase: hsa_circ_0066426, circAtlas: hsa-CADPS_0001
Human Ortholog IDs:
Mature spliced Sequence:
AACCAGGATTGCATTTGAAGTTAAGCTGCAAAAAACCAGTCGATCAACAGATTTTCGAGTCCCACAGTCAATATGCACCATGTTTAATGTTATGGTTGATGCCAAAGCTCAATCAACAAAACTTTGCAGCATGGAAATGGGCCAAGAGCATCAATACCATTCAAAAATAGATGAACTAATTGAAGAAACCGTTAAAGAAATGATCACACTTTTGGTGGCAAAGTTTGTAACTATATTGGAAGGCGTACTGGCAAAGTTATCCAGATATGACGAGGGGACTTTGTTTTCTTCGTTTCTGTCATTCACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCGTACTGGCAAAGTTATCC
59.33
TGGTGCATATTGACTGTGGGA
59.37
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCGTACTGGCAAAGTTATCC
59.33
GGTGCATATTGACTGTGGGAC
58.98
145
PCR template sequence:
ACTTTTGGTGGCAAAGTTTGTAACTATATTGGAAGGCGTACTGGCAAAGTTATCCAGATATGACGAGGGGACTTTGTTTTCTTCGTTTCTGTCATTCACGAACCAGGATTGCATTTGAAGTTAAGCTGCAAAAAACCAGTCGATCAACAGATTTTCGAGTCCCACAGTCAATATGCACCATGTTTAATGTTATGGTTGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-129-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCTGTCATTCACGAACCAGGATTGCA
siRNA sets
ATCCTGGTTCGTGAATGAC, CAATCCTGGTTCGTGAATG, AATCCTGGTTCGTGAATGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCADPS_307_ORF_1
0.7412
-0.052885577
0.170221482
348
ORF sequence:
ATGACGAGGGGACTTTGTTTTCTTCGTTTCTGTCATTCACGAACCAGGATTGCATTTGAAGTTAAGCTGCAAAAAACCAGTCGATCAACAGATTTTCGAGTCCCACAGTCAATATGCACCATGTTTAATGTTATGGTTGATGCCAAAGCTCAATCAACAAAACTTTGCAGCATGGAAATGGGCCAAGAGCATCAATACCATTCAAAAATAGATGAACTAATTGAAGAAACCGTTAAAGAAATGATCACACTTTTGGTGGCAAAGTTTGTAACTATATTGGAAGGCGTACTGGCAAAGTTATCCAGATATGACGAGGGGACTTTGTTTTCTTCGTTTCTGTCATTCACG
Peptide sequence:
MTRGLCFLRFCHSRTRIAFEVKLQKTSRSTDFRVPQSICTMFNVMVDAKAQSTKLCSMEMGQEHQYHSKIDELIEETVKEMITLLVAKFVTILEGVLAKLSRYDEGTLFSSFLSFT
IRESfinder result
IRESfinder score
0.652788689
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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