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mmu_circCacna2d1_2805
Basic information of circular RNA
PanCircBase ID:
mmu_circCacna2d1_2805
mm39_Chr. Position ID:
chr5|16399752|16570871|+
Gene name:
Cacna2d1
Transcript:
ENSMUST00000167946.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
34
Exon/intron indexes:
5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38
Exon/intron offsets:
0,18121,51911,69503,71932,72621,105174,107538,117746,118887,120151,122880,124483,125329,131557,133218,139009,145952,147797,147920,154657,157836,159068,159807,160740,162049,162534,163011,164176,164429,167065,167510,170604,171036
Genomic length:
171119
Exon/intron sizes in nt:
42,130,132,70,51,100,159,105,79,50,90,78,75,75,72,72,62,81,9,69,98,88,63,104,87,68,39,72,153,53,56,130,110,83
Flanking exon/introns:
chr5:16368050-16399752|chr5:16570871-16575555
Splice length:
2805
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
AGCAATGAAGTTGTCTACTATAATGCTAAGGATGATCTTGATCCTGAAAGAAATGAGAGTGAGCCAGGCAGCCAACGGATTAAACCTGTTTTTATTGAAGATGCTAATTTTGGACGTCAGATATCCTATCAGCATGCAGCGGTCCATATTCCCACGGACATCTATGAGGGCTCAACCATAGTGTTAAACGAACTCAACTGGACAAGTGCCTTAGATGAAGTATTCAAAAGAAATCGAGACGAAGACCCTACACTGCTGTGGCAAGTGTTTGGCAGCGCCACTGGCCTGGCCCGATATTATCCAGCTTCTCCATGGGTGGATAATAGTAGAACTCCAAACAAGATTGATCTATATGATGTACGCAGAAGACCATGGTACATCCAGGGAGCTGCATCCCCCAAGGACATGCTCATTCTGGTGGACGTGAGTGGAAGTGTGAGCGGATTGACTCTGAAACTCATCCGAACATCTGTCTCCGAGATGTTAGAAACCCTCTCTGATGATGATTTCGTGAATGTAGCTTCATTTAACAGCAACGCTCAGGATGTAAGCTGTTTCCAGCACCTGGTTCAAGCGAATGTAAGAAATAAGAAGGTGTTGAAAGATGCCGTGAATAACATTACAGCAAAGGGGATCACAGATTACAAGAAAGGCTTTAGCTTTGCCTTCGAACAGCTACTTAATTATAATGTTTCCAGAGCTAATTGCAATAAGATTATCATGTTATTCACGGATGGAGGAGAAGAGAGAGCCCAGGAGATATTTGCCAAATACAATAAAGACAAAAAAGTCCGTGTGTTTACATTTTCCGTCGGTCAACATAATTATGACAGAGGACCTATTCAGTGGATGGCTTGTGAAAATAAAGGTTACTATTATGAGATTCCCTCCATTGGTGCAATAAGAATCAATACTCAGGAATATCTAGATGTTCTGGGAAGACCAATGGTTTTAGCTGGTGACAAAGCGAAGCAAGTTCAATGGACAAATGTGTATTTGGACGCCCTGGAACTGGGACTTGTCATTACTGGAACTCTACCAGTCTTCAACGTCACTGGCCAATCTGAAAATAAGACAAACTTGAAGAACCAGTTGATTCTTGGTGTGATGGGCGTTGATGTGTCTCTGGAAGATATCAAGAGATTGACACCACGTTTTACACTCTGTCCCAATGGCTACTATTTTGCAATTGATCCTAATGGCTATGTCTTATTGCATCCAAATCTTCAGCCAAAGAACCCCAAATCTCAGGAGCCAGTCACACTGGATTTTCTCGATGCTGAGTTAGAGAATGAAATTAAAGTGGAGATTCGAAATAAAATGATAGATGGAGAAAGTGGAGAAAAAACGTTCAGAACTCTGGTCAAGTCTCAAGATGAGAGATACATTGACAAAGGAAATCGAACATACACATGGACGCCTGTCAATGGCACAGATTACAGTTTGGCCTTGGTATTGCCAACCTACAGTTTTTACTATATAAAAGCCAAACTAGAAGAGACAATAACTCAGGCCAGATGTAACTTGGGGATATTTTCAGATTCAGAAACCCTGAAGCCAGACAATTTTGAAGAATCTGGCTATACTTTCATAGCACCAAGGGAATACTGCAATGATCTTAAACCTTCAGATAATAACACTGAATTTCTTTTGAATTTCAATGAATTTATTGATAGGAAAACTCCAAACAACCCCTCCTGTAATACAGATTTGATTAATAGAATCTTGCTGGATGCAGGTTTTACAAATGAACTTGTCCAAAATTATTGGAGTAAGCAGAAAAATATCAAAGGAGTGAAGGCACGCTTTGTGGTGACAGACGGTGGGATTACGAGAGTTTATCCCAAAGAGGCCGGAGAAAATTGGCAAGAGAACCCAGAGACGTACGAGGACAGCTTCTACAAACGGAGCCTAGATAACGATAACTACGTTTTCACTGCGCCCTACTTTAACAAAAGTGGACCTGGTGCGTATGAATCTGGAATTATGGTAAGCAAAGCAGTAGAACTGTATATCCAAGGAAAACTTCTTAAGCCCGCAGTTGTGGGAATTAAAATTGATGTAAATTCTTGGATAGAAAATTTTACCAAAACTTCAATCAGGGATCCGTGTGCTGGTCCAGTTTGTGACTGCAAAAGAAACAGTGATGTAATGGACTGTGTCATTCTAGATGATGGTGGATTTCTTTTGATGGCAAATCACGATGATTACACTAATCAGATTGGACGTTTTTTTGGAGAGATTGACCCAAGCATGATGAGACACCTGGTTAATATATCACTTTATGCATTCAACAAATCATATGACTATCAGTCCGTGTGCGATCCAGGGGCAGCACCAAAGCAGGGGGCAGGACATCGCTCAGCATATGTGCCATCGATTGCAGATATACTGCAGATTGGCTGGTGGGCCACCGCTGCCGCCTGGTCTATTCTCCAGCAGCTGCTCTTGAGTTTGACATTTCCACGGCTCCTTGAGGCAGTTGAGATGGAGGAAGATGACTTCACAGCCTCCCTGTCTAAGCAGAGCTGCATCACAGAACAAACCCAGTACTTCTTCAAGAACGATACTAAATCATTCAGTGGTTTACTGGACTGTGGAAACTGTTCCAGGATCTTTCATGTAGAGAAACTTATGAACACCAACTTAGTATTCATAATGGTGGAGAGCAAAGGGACATGTCCGTGTGACACGCGGCTGCTCATGCAAGCGGAACAGACTTCTGATGGTCCAGATCCTTGCGACATGGTCAAGCAGCCCAGATACCGAAAAGGACCTGATGTCTGCTTTGATAATAATGTGCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGATCCTTGCGACATGGT
60.11
GGTTTAATCCGTTGGCTGCC
59.83
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAAGCAGCCCAGATACCGAA
60.11
GGTTTAATCCGTTGGCTGCC
59.83
141
PCR template sequence:
GCAAGCGGAACAGACTTCTGATGGTCCAGATCCTTGCGACATGGTCAAGCAGCCCAGATACCGAAAAGGACCTGATGTCTGCTTTGATAATAATGTGCTGAGCAATGAAGTTGTCTACTATAATGCTAAGGATGATCTTGATCCTGAAAGAAATGAGAGTGAGCCAGGCAGCCAACGGATTAAACCTGTTTTTATTGAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
27
Interacting miRNAs (#binding sites)
mmu-miR-5101(2), mmu-miR-9-5p(1), mmu-miR-134-5p(1), mmu-miR-199a-5p(1), mmu-miR-30e-5p(1), mmu-miR-122-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-335-5p(1), mmu-miR-30a-5p(1), mmu-miR-216a-5p(1), mmu-miR-326-3p(1), mmu-miR-30d-5p(1), mmu-miR-149-5p(1), mmu-miR-127-3p(1), mmu-miR-676-3p(1), mmu-miR-382-3p(1), mmu-miR-182-5p(1), mmu-miR-425-5p(1), mmu-miR-297a-5p(1), mmu-miR-680(1), mmu-miR-207(1), mmu-miR-188-3p(1), mmu-miR-350-3p(1), mmu-miR-670-5p(1), mmu-miR-697(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TAATAATGTGCTGAGCAATGAAGTTG
siRNA sets
AACTTCATTGCTCAGCACA, CAACTTCATTGCTCAGCAC, ATTGCTCAGCACATTATTA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCACNA2D1_2805_ORF_1
0.925
0.132854117
1
5205
ORF sequence:
ATGCTCATTCTGGTGGACGTGAGTGGAAGTGTGAGCGGATTGACTCTGAAACTCATCCGAACATCTGTCTCCGAGATGTTAGAAACCCTCTCTGATGATGATTTCGTGAATGTAGCTTCATTTAACAGCAACGCTCAGGATGTAAGCTGTTTCCAGCACCTGGTTCAAGCGAATGTAAGAAATAAGAAGGTGTTGAAAGATGCCGTGAATAACATTACAGCAAAGGGGATCACAGATTACAAGAAAGGCTTTAGCTTTGCCTTCGAACAGCTACTTAATTATAATGTTTCCAGAGCTAATTGCAATAAGATTATCATGTTATTCACGGATGGAGGAGAAGAGAGAGCCCAGGAGATATTTGCCAAATACAATAAAGACAAAAAAGTCCGTGTGTTTACATTTTCCGTCGGTCAACATAATTATGACAGAGGACCTATTCAGTGGATGGCTTGTGAAAATAAAGGTTACTATTATGAGATTCCCTCCATTGGTGCAATAAGAATCAATACTCAGGAATATCTAGATGTTCTGGGAAGACCAATGGTTTTAGCTGGTGACAAAGCGAAGCAAGTTCAATGGACAAATGTGTATTTGGACGCCCTGGAACTGGGACTTGTCATTACTGGAACTCTACCAGTCTTCAACGTCACTGGCCAATCTGAAAATAAGACAAACTTGAAGAACCAGTTGATTCTTGGTGTGATGGGCGTTGATGTGTCTCTGGAAGATATCAAGAGATTGACACCACGTTTTACACTCTGTCCCAATGGCTACTATTTTGCAATTGATCCTAATGGCTATGTCTTATTGCATCCAAATCTTCAGCCAAAGAACCCCAAATCTCAGGAGCCAGTCACACTGGATTTTCTCGATGCTGAGTTAGAGAATGAAATTAAAGTGGAGATTCGAAATAAAATGATAGATGGAGAAAGTGGAGAAAAAACGTTCAGAACTCTGGTCAAGTCTCAAGATGAGAGATACATTGACAAAGGAAATCGAACATACACATGGACGCCTGTCAATGGCACAGATTACAGTTTGGCCTTGGTATTGCCAACCTACAGTTTTTACTATATAAAAGCCAAACTAGAAGAGACAATAACTCAGGCCAGATGTAACTTGGGGATATTTTCAGATTCAGAAACCCTGAAGCCAGACAATTTTGAAGAATCTGGCTATACTTTCATAGCACCAAGGGAATACTGCAATGATCTTAAACCTTCAGATAATAACACTGAATTTCTTTTGAATTTCAATGAATTTATTGATAGGAAAACTCCAAACAACCCCTCCTGTAATACAGATTTGATTAATAGAATCTTGCTGGATGCAGGTTTTACAAATGAACTTGTCCAAAATTATTGGAGTAAGCAGAAAAATATCAAAGGAGTGAAGGCACGCTTTGTGGTGACAGACGGTGGGATTACGAGAGTTTATCCCAAAGAGGCCGGAGAAAATTGGCAAGAGAACCCAGAGACGTACGAGGACAGCTTCTACAAACGGAGCCTAGATAACGATAACTACGTTTTCACTGCGCCCTACTTTAACAAAAGTGGACCTGGTGCGTATGAATCTGGAATTATGGTAAGCAAAGCAGTAGAACTGTATATCCAAGGAAAACTTCTTAAGCCCGCAGTTGTGGGAATTAAAATTGATGTAAATTCTTGGATAGAAAATTTTACCAAAACTTCAATCAGGGATCCGTGTGCTGGTCCAGTTTGTGACTGCAAAAGAAACAGTGATGTAATGGACTGTGTCATTCTAGATGATGGTGGATTTCTTTTGATGGCAAATCACGATGATTACACTAATCAGATTGGACGTTTTTTTGGAGAGATTGACCCAAGCATGATGAGACACCTGGTTAATATATCACTTTATGCATTCAACAAATCATATGACTATCAGTCCGTGTGCGATCCAGGGGCAGCACCAAAGCAGGGGGCAGGACATCGCTCAGCATATGTGCCATCGATTGCAGATATACTGCAGATTGGCTGGTGGGCCACCGCTGCCGCCTGGTCTATTCTCCAGCAGCTGCTCTTGAGTTTGACATTTCCACGGCTCCTTGAGGCAGTTGAGATGGAGGAAGATGACTTCACAGCCTCCCTGTCTAAGCAGAGCTGCATCACAGAACAAACCCAGTACTTCTTCAAGAACGATACTAAATCATTCAGTGGTTTACTGGACTGTGGAAACTGTTCCAGGATCTTTCATGTAGAGAAACTTATGAACACCAACTTAGTATTCATAATGGTGGAGAGCAAAGGGACATGTCCGTGTGACACGCGGCTGCTCATGCAAGCGGAACAGACTTCTGATGGTCCAGATCCTTGCGACATGGTCAAGCAGCCCAGATACCGAAAAGGACCTGATGTCTGCTTTGATAATAATGTGCTGAGCAATGAAGTTGTCTACTATAATGCTAAGGATGATCTTGATCCTGAAAGAAATGAGAGTGAGCCAGGCAGCCAACGGATTAAACCTGTTTTTATTGAAGATGCTAATTTTGGACGTCAGATATCCTATCAGCATGCAGCGGTCCATATTCCCACGGACATCTATGAGGGCTCAACCATAGTGTTAAACGAACTCAACTGGACAAGTGCCTTAGATGAAGTATTCAAAAGAAATCGAGACGAAGACCCTACACTGCTGTGGCAAGTGTTTGGCAGCGCCACTGGCCTGGCCCGATATTATCCAGCTTCTCCATGGGTGGATAATAGTAGAACTCCAAACAAGATTGATCTATATGATGTACGCAGAAGACCATGGTACATCCAGGGAGCTGCATCCCCCAAGGACATGCTCATTCTGGTGGACGTGAGTGGAAGTGTGAGCGGATTGACTCTGAAACTCATCCGAACATCTGTCTCCGAGATGTTAGAAACCCTCTCTGATGATGATTTCGTGAATGTAGCTTCATTTAACAGCAACGCTCAGGATGTAAGCTGTTTCCAGCACCTGGTTCAAGCGAATGTAAGAAATAAGAAGGTGTTGAAAGATGCCGTGAATAACATTACAGCAAAGGGGATCACAGATTACAAGAAAGGCTTTAGCTTTGCCTTCGAACAGCTACTTAATTATAATGTTTCCAGAGCTAATTGCAATAAGATTATCATGTTATTCACGGATGGAGGAGAAGAGAGAGCCCAGGAGATATTTGCCAAATACAATAAAGACAAAAAAGTCCGTGTGTTTACATTTTCCGTCGGTCAACATAATTATGACAGAGGACCTATTCAGTGGATGGCTTGTGAAAATAAAGGTTACTATTATGAGATTCCCTCCATTGGTGCAATAAGAATCAATACTCAGGAATATCTAGATGTTCTGGGAAGACCAATGGTTTTAGCTGGTGACAAAGCGAAGCAAGTTCAATGGACAAATGTGTATTTGGACGCCCTGGAACTGGGACTTGTCATTACTGGAACTCTACCAGTCTTCAACGTCACTGGCCAATCTGAAAATAAGACAAACTTGAAGAACCAGTTGATTCTTGGTGTGATGGGCGTTGATGTGTCTCTGGAAGATATCAAGAGATTGACACCACGTTTTACACTCTGTCCCAATGGCTACTATTTTGCAATTGATCCTAATGGCTATGTCTTATTGCATCCAAATCTTCAGCCAAAGAACCCCAAATCTCAGGAGCCAGTCACACTGGATTTTCTCGATGCTGAGTTAGAGAATGAAATTAAAGTGGAGATTCGAAATAAAATGATAGATGGAGAAAGTGGAGAAAAAACGTTCAGAACTCTGGTCAAGTCTCAAGATGAGAGATACATTGACAAAGGAAATCGAACATACACATGGACGCCTGTCAATGGCACAGATTACAGTTTGGCCTTGGTATTGCCAACCTACAGTTTTTACTATATAAAAGCCAAACTAGAAGAGACAATAACTCAGGCCAGATGTAACTTGGGGATATTTTCAGATTCAGAAACCCTGAAGCCAGACAATTTTGAAGAATCTGGCTATACTTTCATAGCACCAAGGGAATACTGCAATGATCTTAAACCTTCAGATAATAACACTGAATTTCTTTTGAATTTCAATGAATTTATTGATAGGAAAACTCCAAACAACCCCTCCTGTAATACAGATTTGATTAATAGAATCTTGCTGGATGCAGGTTTTACAAATGAACTTGTCCAAAATTATTGGAGTAAGCAGAAAAATATCAAAGGAGTGAAGGCACGCTTTGTGGTGACAGACGGTGGGATTACGAGAGTTTATCCCAAAGAGGCCGGAGAAAATTGGCAAGAGAACCCAGAGACGTACGAGGACAGCTTCTACAAACGGAGCCTAGATAACGATAACTACGTTTTCACTGCGCCCTACTTTAACAAAAGTGGACCTGGTGCGTATGAATCTGGAATTATGGTAAGCAAAGCAGTAGAACTGTATATCCAAGGAAAACTTCTTAAGCCCGCAGTTGTGGGAATTAAAATTGATGTAAATTCTTGGATAGAAAATTTTACCAAAACTTCAATCAGGGATCCGTGTGCTGGTCCAGTTTGTGACTGCAAAAGAAACAGTGATGTAATGGACTGTGTCATTCTAGATGATGGTGGATTTCTTTTGATGGCAAATCACGATGATTACACTAATCAGATTGGACGTTTTTTTGGAGAGATTGACCCAAGCATGATGAGACACCTGGTTAATATATCACTTTATGCATTCAACAAATCATATGACTATCAGTCCGTGTGCGATCCAGGGGCAGCACCAAAGCAGGGGGCAGGACATCGCTCAGCATATGTGCCATCGATTGCAGATATACTGCAGATTGGCTGGTGGGCCACCGCTGCCGCCTGGTCTATTCTCCAGCAGCTGCTCTTGAGTTTGACATTTCCACGGCTCCTTGAGGCAGTTGAGATGGAGGAAGATGACTTCACAGCCTCCCTGTCTAAGCAGAGCTGCATCACAGAACAAACCCAGTACTTCTTCAAGAACGATACTAAATCATTCAGTGGTTTACTGGACTGTGGAAACTGTTCCAGGATCTTTCATGTAGAGAAACTTATGAACACCAACTTAGTATTCATAATGGTGGAGAGCAAAGGGACATGTCCGTGTGACACGCGGCTGCTCATGCAAGCGGAACAGACTTCTGATGGTCCAGATCCTTGCGACATGGTCAAGCAGCCCAGATACCGAAAAGGACCTGATGTCTGCTTTGATAATAATGTGCTG
Peptide sequence:
MLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKNPKSQEPVTLDFLDAELENEIKVEIRNKMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQARCNLGIFSDSETLKPDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRILLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVELYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAPKQGAGHRSAYVPSIADILQIGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQYFFKNDTKSFSGLLDCGNCSRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNVLSNEVVYYNAKDDLDPERNESEPGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKRNRDEDPTLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKNPKSQEPVTLDFLDAELENEIKVEIRNKMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQARCNLGIFSDSETLKPDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRILLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVELYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAPKQGAGHRSAYVPSIADILQIGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQYFFKNDTKSFSGLLDCGNCSRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNVL
IRESfinder result
IRESfinder score
0.605485653
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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