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mmu_circCacna1a_367
Basic information of circular RNA
PanCircBase ID:
mmu_circCacna1a_367
mm39_Chr. Position ID:
chr8|85310187|85313838|+
Gene name:
Cacna1a
Transcript:
ENSMUST00000238701.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
30,31
Exon/intron offsets:
0,3486
Genomic length:
3651
Exon/intron sizes in nt:
202,165
Flanking exon/introns:
chr8:85307980-85310187|chr8:85313838-85315377
Splice length:
367
Other database IDs:
circAtlas: hsa-CAC1A_0030
Human Ortholog IDs:
Mature spliced Sequence:
GGTCCTCAAGCACTCAGTGGATGCTACTTTTGAGAACCAGGGCCCCAGCCCCGGGTACCGCATGGAGATGTCCATCTTCTACGTGGTGTACTTTGTAGTGTTTCCCTTCTTCTTTGTCAATATCTTCGTGGCCTTGATCATCATCACCTTTCAGGAGCAGGGAGACAAGATGATGGAAGAGTACAGCCTGGAGAAAAATGAGAGGGCCTGCATCGACTTTGCCATCAGTGCCAAGCCGCTGACCAGGCACATGCCCCAGAACAAGCAGAGCTTCCAGTATCGAATGTGGCAGTTCGTCGTGTCCCCACCCTTTGAGTACACCATCATGGCCATGATCGCTCTCAACACCATCGTGCTAATGATGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAATGTGGCAGTTCGTCGTG
59.84
GACATCTCCATGCGGTACCC
60.25
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAATGTGGCAGTTCGTCGTG
59.84
GGACATCTCCATGCGGTACC
60.25
159
PCR template sequence:
GAGCTTCCAGTATCGAATGTGGCAGTTCGTCGTGTCCCCACCCTTTGAGTACACCATCATGGCCATGATCGCTCTCAACACCATCGTGCTAATGATGAAGGGTCCTCAAGCACTCAGTGGATGCTACTTTTGAGAACCAGGGCCCCAGCCCCGGGTACCGCATGGAGATGTCCATCTTCTACGTGGTGTACTTTGTAGTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-222-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
GCTAATGATGAAGGGTCCTCAAGCAC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCACNA1A_367_ORF_1
1.2987
0.49406666
0.834962175
339
ORF sequence:
ATGGAGATGTCCATCTTCTACGTGGTGTACTTTGTAGTGTTTCCCTTCTTCTTTGTCAATATCTTCGTGGCCTTGATCATCATCACCTTTCAGGAGCAGGGAGACAAGATGATGGAAGAGTACAGCCTGGAGAAAAATGAGAGGGCCTGCATCGACTTTGCCATCAGTGCCAAGCCGCTGACCAGGCACATGCCCCAGAACAAGCAGAGCTTCCAGTATCGAATGTGGCAGTTCGTCGTGTCCCCACCCTTTGAGTACACCATCATGGCCATGATCGCTCTCAACACCATCGTGCTAATGATGAAGGGTCCTCAAGCACTCAGTGGATGCTACTTTTGA
Peptide sequence:
MEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKGPQALSGCYF
IRESfinder result
IRESfinder score
0.451107781
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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