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mmu_circBtbd9_841

Basic information of circular RNA
PanCircBase ID:

mmu_circBtbd9_841

mm39_Chr. Position ID:

chr17|30743686|30749339|-

Gene name:

Btbd9

Transcript:

ENSMUST00000168787.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

3

Exon/intron indexes:

4,3,2

Exon/intron offsets:

0,2589,5441

Genomic length:

5653

Exon/intron sizes in nt:

265,364,212

Flanking exon/introns:

chr17:30736297-30743686|chr17:30749339-30795119

Splice length:

841

Other database IDs:

circBase: mmu_circ_0006681, CIRCpedia: MMU_CIRCpedia_15826, circAtlas: mmu-Btbd9_0002

circBase: hsa_circ_0076255, circAtlas: hsa-BTBD9_0002, CIRCpedia: HSA_CIRCpedia_54919

Human Ortholog IDs:
Mature spliced Sequence:

GCCAGTGACTATCATCTGTTCCACAGGATGAGTAACAGCCACCCTCTTCGCCCCTTTACTGCAGTGGGGGAAATCGATCATGTGCACATTTTGTCTGAGCATATTGGTGCCTTGTTGATTGGTGAAGAATATGGTGATGTCACGTTTGTTGTGGAAAAGAAGCACTTTCCTGCCCACAGGGTAATTTTAGCAGCGAGGTGCCAATATTTTCGAGCATTACTGTATGGCGGGATGCGAGAGTCGCAGCCCGAAGCAGAGATCCCTCTCCAAGACACCACGGCCGAGGCTTTCACGATGCTCCTCAGATATATCTACACGGGGCGGGCGACGCTCACTGATGAGAAGGAGGAGGTGCTGCTGGACTTCCTGAGCCTGGCTCACAAATACGGATTTCCAGAGCTGGAGGACTCTACTTCTGAGTATCTCTGCACCATACTGAACATTCAGAATGTCTGTATGACCTTTGATGTTGCCAGTCTCTACTCGCTTCCCAAGTTAACTTGTATGTGCTGCATGTTCATGGACAGAAATGCTCAGGAGGTGCTGGCCAGCGACGGCTTCCTCTCCCTGTCCAAGACAGCACTCCTGAATATCGTCTTAAGAGATTCGTTTGCTGCTCCTGAGAAAGATATTTTCTTAGCCTTGTTAAACTGGTGTAAGCACAATGCAAAAGAGAATCATGCTGAAATCATGCAGGCTGTGCGCTTACCTCTAATGAGCCTCACTGAGCTTCTGAACGTCGTGAGGCCATCAGGACTCTTGTCTCCGGATGCTATTCTTGATGCCATCAAAGTGCGGTCGGAGAGCCGGGATATGGACCTCAACTACAGAGGCATGCTCA

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GATGCCATCAAAGTGCGGTC

59.9

GATCGATTTCCCCCACTGCA

60.11

140

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GATGCCATCAAAGTGCGGTC

59.9

TGATCGATTTCCCCCACTGC

60.11

141

PCR template sequence:

GTGAGGCCATCAGGACTCTTGTCTCCGGATGCTATTCTTGATGCCATCAAAGTGCGGTCGGAGAGCCGGGATATGGACCTCAACTACAGAGGCATGCTCAGCCAGTGACTATCATCTGTTCCACAGGATGAGTAACAGCCACCCTCTTCGCCCCTTTACTGCAGTGGGGGAAATCGATCATGTGCACATTTTGTCTGAGC

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

16

Interacting miRNAs (#binding sites)

mmu-miR-15a-5p(3), mmu-miR-322-5p(2), mmu-miR-15b-5p(2), mmu-miR-20b-5p(1), mmu-miR-16-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-33-5p(1), mmu-miR-324-5p(1), mmu-miR-10a-5p(1), mmu-miR-495-3p(1), mmu-miR-24-3p(1), mmu-miR-127-5p(1), mmu-miR-182-5p(1), mmu-miR-484(1)

siRNAs for circular RNA silencing
Junction sequence

AGAGGCATGCTCAGCCAGTGACTATC

siRNA sets

GATAGTCACTGGCTGAGCA, ATAGTCACTGGCTGAGCAT

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBTBD9_841_ORF_1

1.076

0.212949496

0.984790469

912

ORF sequence:

ATGCTATTCTTGATGCCATCAAAGTGCGGTCGGAGAGCCGGGATATGGACCTCAACTACAGAGGCATGCTCAGCCAGTGACTATCATCTGTTCCACAGGATGAGTAACAGCCACCCTCTTCGCCCCTTTACTGCAGTGGGGGAAATCGATCATGTGCACATTTTGTCTGAGCATATTGGTGCCTTGTTGATTGGTGAAGAATATGGTGATGTCACGTTTGTTGTGGAAAAGAAGCACTTTCCTGCCCACAGGGTAATTTTAGCAGCGAGGTGCCAATATTTTCGAGCATTACTGTATGGCGGGATGCGAGAGTCGCAGCCCGAAGCAGAGATCCCTCTCCAAGACACCACGGCCGAGGCTTTCACGATGCTCCTCAGATATATCTACACGGGGCGGGCGACGCTCACTGATGAGAAGGAGGAGGTGCTGCTGGACTTCCTGAGCCTGGCTCACAAATACGGATTTCCAGAGCTGGAGGACTCTACTTCTGAGTATCTCTGCACCATACTGAACATTCAGAATGTCTGTATGACCTTTGATGTTGCCAGTCTCTACTCGCTTCCCAAGTTAACTTGTATGTGCTGCATGTTCATGGACAGAAATGCTCAGGAGGTGCTGGCCAGCGACGGCTTCCTCTCCCTGTCCAAGACAGCACTCCTGAATATCGTCTTAAGAGATTCGTTTGCTGCTCCTGAGAAAGATATTTTCTTAGCCTTGTTAAACTGGTGTAAGCACAATGCAAAAGAGAATCATGCTGAAATCATGCAGGCTGTGCGCTTACCTCTAATGAGCCTCACTGAGCTTCTGAACGTCGTGAGGCCATCAGGACTCTTGTCTCCGGATGCTATTCTTGATGCCATCAAAGTGCGGTCGGAGAGCCGGGATATGGACCTCAACTACAGAGGCATGCTC

Peptide sequence:

MLFLMPSKCGRRAGIWTSTTEACSASDYHLFHRMSNSHPLRPFTAVGEIDHVHILSEHIGALLIGEEYGDVTFVVEKKHFPAHRVILAARCQYFRALLYGGMRESQPEAEIPLQDTTAEAFTMLLRYIYTGRATLTDEKEEVLLDFLSLAHKYGFPELEDSTSEYLCTILNIQNVCMTFDVASLYSLPKLTCMCCMFMDRNAQEVLASDGFLSLSKTALLNIVLRDSFAAPEKDIFLALLNWCKHNAKENHAEIMQAVRLPLMSLTELLNVVRPSGLLSPDAILDAIKVRSESRDMDLNYRGML

IRESfinder result

IRESfinder score

0.437219285

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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