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mmu_circBtbd9_1291
Basic information of circular RNA
PanCircBase ID:
mmu_circBtbd9_1291
mm39_Chr. Position ID:
chr17|30553196|30749339|-
Gene name:
Btbd9
Transcript:
ENSMUST00000168787.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
7,6,5,4,3,2
Exon/intron offsets:
0,179333,182881,190490,193079,195931
Genomic length:
196143
Exon/intron sizes in nt:
1,10,12,02,20,26,53,64,000
Flanking exon/introns:
chr17:30518694-30553196|chr17:30749339-30795119
Splice length:
1291
Other database IDs:
circAtlas: mmu-Btbd9_0021
Human Ortholog IDs:
Mature spliced Sequence:
GCCAGTGACTATCATCTGTTCCACAGGATGAGTAACAGCCACCCTCTTCGCCCCTTTACTGCAGTGGGGGAAATCGATCATGTGCACATTTTGTCTGAGCATATTGGTGCCTTGTTGATTGGTGAAGAATATGGTGATGTCACGTTTGTTGTGGAAAAGAAGCACTTTCCTGCCCACAGGGTAATTTTAGCAGCGAGGTGCCAATATTTTCGAGCATTACTGTATGGCGGGATGCGAGAGTCGCAGCCCGAAGCAGAGATCCCTCTCCAAGACACCACGGCCGAGGCTTTCACGATGCTCCTCAGATATATCTACACGGGGCGGGCGACGCTCACTGATGAGAAGGAGGAGGTGCTGCTGGACTTCCTGAGCCTGGCTCACAAATACGGATTTCCAGAGCTGGAGGACTCTACTTCTGAGTATCTCTGCACCATACTGAACATTCAGAATGTCTGTATGACCTTTGATGTTGCCAGTCTCTACTCGCTTCCCAAGTTAACTTGTATGTGCTGCATGTTCATGGACAGAAATGCTCAGGAGGTGCTGGCCAGCGACGGCTTCCTCTCCCTGTCCAAGACAGCACTCCTGAATATCGTCTTAAGAGATTCGTTTGCTGCTCCTGAGAAAGATATTTTCTTAGCCTTGTTAAACTGGTGTAAGCACAATGCAAAAGAGAATCATGCTGAAATCATGCAGGCTGTGCGCTTACCTCTAATGAGCCTCACTGAGCTTCTGAACGTCGTGAGGCCATCAGGACTCTTGTCTCCGGATGCTATTCTTGATGCCATCAAAGTGCGGTCGGAGAGCCGGGATATGGACCTCAACTACAGAGGCATGCTCATACCTGAAGAAAACATTGCAACTATGAAGTATGGGGCTCAGGTTGTGAAAGGAGAGCTGAAGTCGGCCTTGCTGGATGGTGACACTCAAAACTATGACTTGGATCATGGATTTTCCCGGCACCCCATTGATGATGACTGCCGCTCTGGCATTGAGATCAAGCTTGGCCAGCCGTCCATTATAAACCACATACGTCTACTCCTGTGGGACCGTGACAGCCGGTCTTATTCCTACTTTATTGAAGTGTCCATGGACGAACTTGATTGGATCAGAGTGATCGATCACTCACATTACCTGTGTCGGTCTTGGCAAAAGTTGTATTTCCCAGCCCGTGTCTGCAGGTATATCCGAATAGTTGGGACTCACAACACAGTGAACAAGATTTTCCACATTGTGGCTTTTGAATGCATGTTTACAAACAAAGCCTTCACCCTGGAGAAGGGCCTGATAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGGACTCACAACACAGTGA
58.8
TGCAGTAAAGGGGCGAAGAG
60.04
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGGACTCACAACACAGTGA
58.8
CTGCAGTAAAGGGGCGAAGA
60.04
159
PCR template sequence:
ATAGTTGGGACTCACAACACAGTGAACAAGATTTTCCACATTGTGGCTTTTGAATGCATGTTTACAAACAAAGCCTTCACCCTGGAGAAGGGCCTGATAGGCCAGTGACTATCATCTGTTCCACAGGATGAGTAACAGCCACCCTCTTCGCCCCTTTACTGCAGTGGGGGAAATCGATCATGTGCACATTTTGTCTGAGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
21
Interacting miRNAs (#binding sites)
mmu-miR-15a-5p(3), mmu-miR-322-5p(2), mmu-miR-15b-5p(2), mmu-miR-20b-5p(1), mmu-miR-16-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-30e-5p(1), mmu-miR-33-5p(1), mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-324-5p(1), mmu-miR-10a-5p(1), mmu-miR-495-3p(1), mmu-miR-24-3p(1), mmu-miR-30b-5p(1), mmu-miR-127-5p(1), mmu-miR-182-5p(1), mmu-miR-342-5p(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
AAGGGCCTGATAGGCCAGTGACTATC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTBD9_1291_ORF_1
1.129
0.240977598
0.999052293
1272
ORF sequence:
ATGAGTAACAGCCACCCTCTTCGCCCCTTTACTGCAGTGGGGGAAATCGATCATGTGCACATTTTGTCTGAGCATATTGGTGCCTTGTTGATTGGTGAAGAATATGGTGATGTCACGTTTGTTGTGGAAAAGAAGCACTTTCCTGCCCACAGGGTAATTTTAGCAGCGAGGTGCCAATATTTTCGAGCATTACTGTATGGCGGGATGCGAGAGTCGCAGCCCGAAGCAGAGATCCCTCTCCAAGACACCACGGCCGAGGCTTTCACGATGCTCCTCAGATATATCTACACGGGGCGGGCGACGCTCACTGATGAGAAGGAGGAGGTGCTGCTGGACTTCCTGAGCCTGGCTCACAAATACGGATTTCCAGAGCTGGAGGACTCTACTTCTGAGTATCTCTGCACCATACTGAACATTCAGAATGTCTGTATGACCTTTGATGTTGCCAGTCTCTACTCGCTTCCCAAGTTAACTTGTATGTGCTGCATGTTCATGGACAGAAATGCTCAGGAGGTGCTGGCCAGCGACGGCTTCCTCTCCCTGTCCAAGACAGCACTCCTGAATATCGTCTTAAGAGATTCGTTTGCTGCTCCTGAGAAAGATATTTTCTTAGCCTTGTTAAACTGGTGTAAGCACAATGCAAAAGAGAATCATGCTGAAATCATGCAGGCTGTGCGCTTACCTCTAATGAGCCTCACTGAGCTTCTGAACGTCGTGAGGCCATCAGGACTCTTGTCTCCGGATGCTATTCTTGATGCCATCAAAGTGCGGTCGGAGAGCCGGGATATGGACCTCAACTACAGAGGCATGCTCATACCTGAAGAAAACATTGCAACTATGAAGTATGGGGCTCAGGTTGTGAAAGGAGAGCTGAAGTCGGCCTTGCTGGATGGTGACACTCAAAACTATGACTTGGATCATGGATTTTCCCGGCACCCCATTGATGATGACTGCCGCTCTGGCATTGAGATCAAGCTTGGCCAGCCGTCCATTATAAACCACATACGTCTACTCCTGTGGGACCGTGACAGCCGGTCTTATTCCTACTTTATTGAAGTGTCCATGGACGAACTTGATTGGATCAGAGTGATCGATCACTCACATTACCTGTGTCGGTCTTGGCAAAAGTTGTATTTCCCAGCCCGTGTCTGCAGGTATATCCGAATAGTTGGGACTCACAACACAGTGAACAAGATTTTCCACATTGTGGCTTTTGAATGCATGTTTACAAACAAAGCCTTCACCCTGGAGAAGGGCCTGATAGGCCAGTGA
Peptide sequence:
MSNSHPLRPFTAVGEIDHVHILSEHIGALLIGEEYGDVTFVVEKKHFPAHRVILAARCQYFRALLYGGMRESQPEAEIPLQDTTAEAFTMLLRYIYTGRATLTDEKEEVLLDFLSLAHKYGFPELEDSTSEYLCTILNIQNVCMTFDVASLYSLPKLTCMCCMFMDRNAQEVLASDGFLSLSKTALLNIVLRDSFAAPEKDIFLALLNWCKHNAKENHAEIMQAVRLPLMSLTELLNVVRPSGLLSPDAILDAIKVRSESRDMDLNYRGMLIPEENIATMKYGAQVVKGELKSALLDGDTQNYDLDHGFSRHPIDDDCRSGIEIKLGQPSIINHIRLLLWDRDSRSYSYFIEVSMDELDWIRVIDHSHYLCRSWQKLYFPARVCRYIRIVGTHNTVNKIFHIVAFECMFTNKAFTLEKGLIGQ
IRESfinder result
IRESfinder score
0.50891907
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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