top of page
mmu_circBtbd8_676
Basic information of circular RNA
PanCircBase ID:
mmu_circBtbd8_676
mm39_Chr. Position ID:
chr5|107651204|107651880|+
Gene name:
Btbd8
Transcript:
ENSMUST00000211896.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
14
Exon/intron offsets:
0
Genomic length:
676
Exon/intron sizes in nt:
676
Flanking exon/introns:
chr5:107647954-107651204|chr5:107651880-107652369
Splice length:
676
Other database IDs:
circAtlas: mmu-A830010M20Rik_0002
Human Ortholog IDs:
Mature spliced Sequence:
CAAAGGAGACAAGGCTCTGATGTTGATGTTTTAAAAATAAAACCTTGGACAGAAAATAACAAAAAACCGTGTCAGACCTCTCTCTCTACTAATCAGAAGATGAGATCCGATGGATTGGGAGCATCAGGACATGCATCAAGCACTAACAGAAACAGTATAAATAAAGTGTCCAAGCATGGTGACAGTACAAAAATGTCCAAAGTTGTGAAAGAGATGAAAACTGGGGGAAAATATGTCTCTGGCAAGTCTAAAACTATGGTAAAGCCCCAAACAGAAAACAGTGATCATACAAAGATAGAAGGTTTGTCATCCACTGTTGTGGGAAGACCATCAAGAGTGACAGCAGCTGGACGGAAGGACCCAGTACATGGAAAAGGAGTCCAGAACCAAGAAGTGGAGACCACAGGGGCAAGACCTAAGGTACTCACTGCAAACCTGAACGTGCAAGCCAGAGCCAAGCCTTTGCAAACACTGAGAGGAAAAGATTCCACATGCCCCGCCTCTGTAGGGCCCTCCAGTAGATCTACACATTCCAGCACGGAGCTTCTGGCTTCCATGGGCTCTGTGGATGAGACAAAGGAAAACGGATCAGTAGAAGACAAGTGTCGTGATGGGAAGCCATACGTTAGTGATTCCCCAGGACAGATGGTGAGCAATGGAGTAATGTCAACTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCGTGATGGGAAGCCATACG
59.9
AGAGAGAGGTCTGACACGGT
59.6
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGTGATGGGAAGCCATACGT
60.18
AGAGAGAGGTCTGACACGGT
59.6
155
PCR template sequence:
AAGGAAAACGGATCAGTAGAAGACAAGTGTCGTGATGGGAAGCCATACGTTAGTGATTCCCCAGGACAGATGGTGAGCAATGGAGTAATGTCAACTGCAGCAAAGGAGACAAGGCTCTGATGTTGATGTTTTAAAAATAAAACCTTGGACAGAAAATAACAAAAAACCGTGTCAGACCTCTCTCTCTACTAATCAGAAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-1a-3p(1), mmu-miR-150-5p(1), mmu-miR-135a-5p(1), mmu-miR-152-3p(1), mmu-miR-673-5p(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATGTCAACTGCAGCAAAGGAGACAAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTBD8_676_ORF_1
0.8158
0.07219253
0.699660974
597
ORF sequence:
ATGAGATCCGATGGATTGGGAGCATCAGGACATGCATCAAGCACTAACAGAAACAGTATAAATAAAGTGTCCAAGCATGGTGACAGTACAAAAATGTCCAAAGTTGTGAAAGAGATGAAAACTGGGGGAAAATATGTCTCTGGCAAGTCTAAAACTATGGTAAAGCCCCAAACAGAAAACAGTGATCATACAAAGATAGAAGGTTTGTCATCCACTGTTGTGGGAAGACCATCAAGAGTGACAGCAGCTGGACGGAAGGACCCAGTACATGGAAAAGGAGTCCAGAACCAAGAAGTGGAGACCACAGGGGCAAGACCTAAGGTACTCACTGCAAACCTGAACGTGCAAGCCAGAGCCAAGCCTTTGCAAACACTGAGAGGAAAAGATTCCACATGCCCCGCCTCTGTAGGGCCCTCCAGTAGATCTACACATTCCAGCACGGAGCTTCTGGCTTCCATGGGCTCTGTGGATGAGACAAAGGAAAACGGATCAGTAGAAGACAAGTGTCGTGATGGGAAGCCATACGTTAGTGATTCCCCAGGACAGATGGTGAGCAATGGAGTAATGTCAACTGCAGCAAAGGAGACAAGGCTCTGA
Peptide sequence:
MRSDGLGASGHASSTNRNSINKVSKHGDSTKMSKVVKEMKTGGKYVSGKSKTMVKPQTENSDHTKIEGLSSTVVGRPSRVTAAGRKDPVHGKGVQNQEVETTGARPKVLTANLNVQARAKPLQTLRGKDSTCPASVGPSSRSTHSSTELLASMGSVDETKENGSVEDKCRDGKPYVSDSPGQMVSNGVMSTAAKETRL
IRESfinder result
IRESfinder score
0.392695288
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page