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mmu_circBtbd7_959

Basic information of circular RNA
PanCircBase ID:

mmu_circBtbd7_959

mm39_Chr. Position ID:

chr12|102756963|102779102|-

Gene name:

Btbd7

Transcript:

ENSMUST00000223554.2

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

6

Exon/intron indexes:

10,9,8,7,6,5

Exon/intron offsets:

0,3041,4468,17195,20415,21930

Genomic length:

22139

Exon/intron sizes in nt:

179,190,144,161,76,209

Flanking exon/introns:

chr12:102754643-102756963|chr12:102779102-102803876

Splice length:

959

Other database IDs:

circBase: mmu_circ_0003672, CIRCpedia: MMU_CIRCpedia_772, circAtlas: mmu-Btbd7_0008

circAtlas: hsa-BTBD7_0008, CIRCpedia: HSA_CIRCpedia_370718

Human Ortholog IDs:
Mature spliced Sequence:

GCTGTGAGGACATCATTGCTGAGAGCATCTCATTAGACACCTTAATTGCCGTCCTCAAGTGGAGCTCGCATCCATACGGCTCTAAGTGGGTGCACCGACAAGCTGTGCATTTCCTGTGTGAGGAGCTATCCCAGGTCATGACTTCGGATGTTTTTTATGAACTGAGCAAAGACCATCTGCTTACTGCTATCCAGTCTGACTACCTACAGGCAAGCGAACAAGATATCCTTAAATATCTGATTAAATGGGGTGAGCATCAGTTGATGAAAAGAATAGCAGACAGAGAGCCAAACTTACTGAGTGGCACTGCACATAGTGTGAACAAAAGAGGTGTGAAGAGAAGAGACCTGGACATAGAAGAACTTAGAGAGATCCTTTCTTCTCTCTTGCCTTTTGTGCGGATTGAACACATCCTACCTATAAACAGTGAGGTCTTAAGTGATGCAATGAAAAGAGGCTTGATCAGCACGCCGCCATCAGACATGCTTCCTACAGCAGAGGGCGGGAAATCAAACGCCTGGCTGCGGCAGAAGAACGCTGGCATATATGTTCGTCCTCGGCTGTTTTCTCCCTATGTGGAGGAAGCAAAGTCAGTGCTGGATGAGATGATGGTGGAACAGACAGACCTGGTGCGACTGCGAATGGTGCGCATGTCCAATGTCCCAGACACACTCTACATGGTCAGCAATGCCATGCCCCAGTGCTGCCACATGATCAGCCACCAGCAGATCAGCAGTAACCAGTCGAGCCCTCCATCAGTTGTAGCCAATGAGATCCCAGTTCCTCGTCTCCTCATTATGAAGGATATGGTGAGGCGCCTGCAGGAACTGCGGCACACAGAGCAGGTGCAGAGAGCCTATGCACTGAACTGTGGGGAAGGTGCCACCGTCAGCTATGAAATCCAGATCCGAGTGCTCAGAGAGTTTGGTCTTGCAGACGCCGCTGCAGAGCTCCTACAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TGCACTGAACTGTGGGGAAG

60.18

CTCCACTTGAGGACGGCAAT

60.04

164

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TGCCACCGTCAGCTATGAAA

59.68

CTCCACTTGAGGACGGCAAT

60.04

143

PCR template sequence:

TGCACTGAACTGTGGGGAAGGTGCCACCGTCAGCTATGAAATCCAGATCCGAGTGCTCAGAGAGTTTGGTCTTGCAGACGCCGCTGCAGAGCTCCTACAGGCTGTGAGGACATCATTGCTGAGAGCATCTCATTAGACACCTTAATTGCCGTCCTCAAGTGGAGCTCGCATCCATACGGCTCTAAGTGGGTGCACCGACA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

11

Interacting miRNAs (#binding sites)

mmu-miR-335-3p(2), mmu-let-7a-5p(1), mmu-miR-375-3p(1), mmu-let-7c-5p(1), mmu-miR-29c-3p(1), mmu-miR-138-5p(1), mmu-let-7d-5p(1), mmu-miR-24-3p(1), mmu-miR-342-3p(1), mmu-miR-300-3p(1), mmu-miR-574-5p(1)

siRNAs for circular RNA silencing
Junction sequence

AGAGCTCCTACAGGCTGTGAGGACAT

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBTBD7_959_ORF_1

1.033

0.264904493

0.980310053

858

ORF sequence:

ATGACTTCGGATGTTTTTTATGAACTGAGCAAAGACCATCTGCTTACTGCTATCCAGTCTGACTACCTACAGGCAAGCGAACAAGATATCCTTAAATATCTGATTAAATGGGGTGAGCATCAGTTGATGAAAAGAATAGCAGACAGAGAGCCAAACTTACTGAGTGGCACTGCACATAGTGTGAACAAAAGAGGTGTGAAGAGAAGAGACCTGGACATAGAAGAACTTAGAGAGATCCTTTCTTCTCTCTTGCCTTTTGTGCGGATTGAACACATCCTACCTATAAACAGTGAGGTCTTAAGTGATGCAATGAAAAGAGGCTTGATCAGCACGCCGCCATCAGACATGCTTCCTACAGCAGAGGGCGGGAAATCAAACGCCTGGCTGCGGCAGAAGAACGCTGGCATATATGTTCGTCCTCGGCTGTTTTCTCCCTATGTGGAGGAAGCAAAGTCAGTGCTGGATGAGATGATGGTGGAACAGACAGACCTGGTGCGACTGCGAATGGTGCGCATGTCCAATGTCCCAGACACACTCTACATGGTCAGCAATGCCATGCCCCAGTGCTGCCACATGATCAGCCACCAGCAGATCAGCAGTAACCAGTCGAGCCCTCCATCAGTTGTAGCCAATGAGATCCCAGTTCCTCGTCTCCTCATTATGAAGGATATGGTGAGGCGCCTGCAGGAACTGCGGCACACAGAGCAGGTGCAGAGAGCCTATGCACTGAACTGTGGGGAAGGTGCCACCGTCAGCTATGAAATCCAGATCCGAGTGCTCAGAGAGTTTGGTCTTGCAGACGCCGCTGCAGAGCTCCTACAGGCTGTGAGGACATCATTGCTGAGAGCATCTCATTAG

Peptide sequence:

MTSDVFYELSKDHLLTAIQSDYLQASEQDILKYLIKWGEHQLMKRIADREPNLLSGTAHSVNKRGVKRRDLDIEELREILSSLLPFVRIEHILPINSEVLSDAMKRGLISTPPSDMLPTAEGGKSNAWLRQKNAGIYVRPRLFSPYVEEAKSVLDEMMVEQTDLVRLRMVRMSNVPDTLYMVSNAMPQCCHMISHQQISSNQSSPPSVVANEIPVPRLLIMKDMVRRLQELRHTEQVQRAYALNCGEGATVSYEIQIRVLREFGLADAAAELLQAVRTSLLRASH

IRESfinder result

IRESfinder score

0.357831277

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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