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mmu_circBtaf1_651
Basic information of circular RNA
PanCircBase ID:
mmu_circBtaf1_651
mm39_Chr. Position ID:
chr19|36971669|36975035|+
Gene name:
Btaf1
Transcript:
ENSMUST00000099494.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
26,27,28,29,30
Exon/intron offsets:
0,321,529,2260,3236
Genomic length:
3366
Exon/intron sizes in nt:
1,53,12,21,68,78,130
Flanking exon/introns:
chr19:36969984-36971669|chr19:36975035-36977491
Splice length:
651
Other database IDs:
CIRCpedia: MMU_CIRCpedia_39315, circAtlas: mmu-Btaf1_0010
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_76612
Human Ortholog IDs:
Mature spliced Sequence:
GCAGGCATTCCAGACCCTCCAAACATGTCAGAAGAATTAATCCAGTTGAAAGCAAAAGAGCGACACTTTTTAGAGCAATTGTTAGATGGGAAGAAGTTAGAAAATTACAAGATCCCAGTGCCAATCAATGCTGAACTCAGGAAATATCAGCAGGATGGTGTGAACTGGTTAGCCTTTCTCAATAAGTATAAGCTTCATGGAATTCTGTGTGATGACATGGGCTTAGGAAAAACATTACAGTCCATCTGCATTCTAGCAGGAGATCACTGTCAGAGGGCCCAGGAATATGCAAGATCGAAATTGGCAGAATGTATGCCACTTCCTTCCTTGGTGGTTTGTCCACCAACATTAACAGGTCATTGGGTAGATGAAGTAGGTAAATTTTGTTCCAGAGAATATCTCAATCCGTTGCATTATACTGGCCCTCCCACTGAAAGGATAAGGTTGCAACATCAAGTAAAAAGGCACAATCTGATAGTAGCCTCATATGATGTTGTGAGGAATGACATAGACTTTTTTAGAAATATTAAATTTAACTACTGTATTCTTGATGAAGGCCATGTCATAAAAAATGGAAAAACCAAGTTGTCCAAAGCAGTAAAGCAGCTGACTGCTAATTACAGGATTATTCTTTCTGGAACACCAATCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AACCAAGTTGTCCAAAGCAGT
58.54
AGTGTCGCTCTTTTGCTTTCA
58.71
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCAAGTTGTCCAAAGCAGT
57.86
AAGTGTCGCTCTTTTGCTTTCA
59.32
140
PCR template sequence:
TGAAGGCCATGTCATAAAAAATGGAAAAACCAAGTTGTCCAAAGCAGTAAAGCAGCTGACTGCTAATTACAGGATTATTCTTTCTGGAACACCAATCCAGGCAGGCATTCCAGACCCTCCAAACATGTCAGAAGAATTAATCCAGTTGAAAGCAAAAGAGCGACACTTTTTAGAGCAATTGTTAGATGGGAAGAAGTTAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-96-5p(1), mmu-miR-690(1)
siRNAs for circular RNA silencing
Junction sequence
AACACCAATCCAGGCAGGCATTCCAG
siRNA sets
GAATGCCTGCCTGGATTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTAF1_651_ORF_1
0.7775
-0.019474459
0.995107582
1278
ORF sequence:
ATGTCAGAAGAATTAATCCAGTTGAAAGCAAAAGAGCGACACTTTTTAGAGCAATTGTTAGATGGGAAGAAGTTAGAAAATTACAAGATCCCAGTGCCAATCAATGCTGAACTCAGGAAATATCAGCAGGATGGTGTGAACTGGTTAGCCTTTCTCAATAAGTATAAGCTTCATGGAATTCTGTGTGATGACATGGGCTTAGGAAAAACATTACAGTCCATCTGCATTCTAGCAGGAGATCACTGTCAGAGGGCCCAGGAATATGCAAGATCGAAATTGGCAGAATGTATGCCACTTCCTTCCTTGGTGGTTTGTCCACCAACATTAACAGGTCATTGGGTAGATGAAGTAGGTAAATTTTGTTCCAGAGAATATCTCAATCCGTTGCATTATACTGGCCCTCCCACTGAAAGGATAAGGTTGCAACATCAAGTAAAAAGGCACAATCTGATAGTAGCCTCATATGATGTTGTGAGGAATGACATAGACTTTTTTAGAAATATTAAATTTAACTACTGTATTCTTGATGAAGGCCATGTCATAAAAAATGGAAAAACCAAGTTGTCCAAAGCAGTAAAGCAGCTGACTGCTAATTACAGGATTATTCTTTCTGGAACACCAATCCAGGCAGGCATTCCAGACCCTCCAAACATGTCAGAAGAATTAATCCAGTTGAAAGCAAAAGAGCGACACTTTTTAGAGCAATTGTTAGATGGGAAGAAGTTAGAAAATTACAAGATCCCAGTGCCAATCAATGCTGAACTCAGGAAATATCAGCAGGATGGTGTGAACTGGTTAGCCTTTCTCAATAAGTATAAGCTTCATGGAATTCTGTGTGATGACATGGGCTTAGGAAAAACATTACAGTCCATCTGCATTCTAGCAGGAGATCACTGTCAGAGGGCCCAGGAATATGCAAGATCGAAATTGGCAGAATGTATGCCACTTCCTTCCTTGGTGGTTTGTCCACCAACATTAACAGGTCATTGGGTAGATGAAGTAGGTAAATTTTGTTCCAGAGAATATCTCAATCCGTTGCATTATACTGGCCCTCCCACTGAAAGGATAAGGTTGCAACATCAAGTAAAAAGGCACAATCTGATAGTAGCCTCATATGATGTTGTGAGGAATGACATAGACTTTTTTAGAAATATTAAATTTAACTACTGTATTCTTGATGAAGGCCATGTCATAAAAAATGGAAAAACCAAGTTGTCCAAAGCAGTAAAGCAGCTGACTGCTAATTACAGGATTATTCTTTCTGGAACACCAATCCAG
Peptide sequence:
MSEELIQLKAKERHFLEQLLDGKKLENYKIPVPINAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCQRAQEYARSKLAECMPLPSLVVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNLIVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPIQAGIPDPPNMSEELIQLKAKERHFLEQLLDGKKLENYKIPVPINAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCQRAQEYARSKLAECMPLPSLVVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNLIVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPIQ
IRESfinder result
IRESfinder score
0.573340803
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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