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mmu_circBtaf1_590
Basic information of circular RNA
PanCircBase ID:
mmu_circBtaf1_590
mm39_Chr. Position ID:
chr19|36933770|36943107|+
Gene name:
Btaf1
Transcript:
ENSMUST00000099494.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
5,6,7,8,9
Exon/intron offsets:
0,2002,5268,6060,9247
Genomic length:
9337
Exon/intron sizes in nt:
164,137,130,69,90
Flanking exon/introns:
chr19:36928577-36933770|chr19:36943107-36943891
Splice length:
590
Other database IDs:
circAtlas: mmu-Btaf1_0001
Human Ortholog IDs:
Mature spliced Sequence:
GTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCTGGGAGGTTCGACATG
59.82
ATTTCCGTTGGCGTGCTATC
58.99
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCTGGGAGGTTCGACATG
59.82
TTTCCGTTGGCGTGCTATCC
61.02
141
PCR template sequence:
CTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-93-5p(1), mmu-miR-106b-5p(1), mmu-miR-217-5p(1), mmu-miR-324-5p(1), mmu-miR-541-5p(1), mmu-miR-5101(1), mmu-miR-1199-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CACTTTAGAGGAGGTGAAGTGGATCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTAF1_590_ORF_1
1.0209
0.084122941
0.717790438
549
ORF sequence:
ATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGGTGAAGTGGATCCTAAAGAGAGGATAG
Peptide sequence:
MGEAIGMSTEELFNDEDLDYTPTSAALVNKQSTLQAAELIDSEFRAGMSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEVKWILKRG
IRESfinder result
IRESfinder score
0.547329774
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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