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mmu_circBtaf1_562
Basic information of circular RNA
PanCircBase ID:
mmu_circBtaf1_562
mm39_Chr. Position ID:
chr19|36939038|36944252|+
Gene name:
Btaf1
Transcript:
ENSMUST00000099494.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
7,8,9,10,11
Exon/intron offsets:
0,792,3979,4853,5037
Genomic length:
5214
Exon/intron sizes in nt:
1,30,69,90,96,177
Flanking exon/introns:
chr19:36935909-36939038|chr19:36944252-36946455
Splice length:
562
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGACAGTGGATGTGCTGCT
59.6
TCCGTCTCTTTTCTTCTGGCT
59.03
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGACAGTGGATGTGCTGCT
59.6
TCCGTCTCTTTTCTTCTGGCTC
60.03
140
PCR template sequence:
TAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-93-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-106b-5p(1), mmu-miR-218-5p(1), mmu-miR-541-5p(1), mmu-miR-1199-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCAGTTCGTCAGCAATGACAGCACT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTAF1_562_ORF_1
1.0149
0.024795047
0.71489008
582
ORF sequence:
ATGCTTTGGCAGTTCGTCAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAG
Peptide sequence:
MLWQFVSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVRQ
IRESfinder result
IRESfinder score
0.59008678
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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