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mmu_circBtaf1_1649
Basic information of circular RNA
PanCircBase ID:
mmu_circBtaf1_1649
mm39_Chr. Position ID:
chr19|36928433|36958126|+
Gene name:
Btaf1
Transcript:
ENSMUST00000099494.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
4,5,6,7,8,9,10,11,12,13,14,15,16
Exon/intron offsets:
0,5337,7339,10605,11397,14584,15458,15642,18022,18880,21864,27281,29543
Genomic length:
29693
Exon/intron sizes in nt:
1,44,16,41,37,13,06,99,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr19:36926582-36928433|chr19:36958126-36958386
Splice length:
1649
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AACCTACAGAGAGTTCTATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTTGGGGTCTGCTGGTGCAGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGGATGTAATTAATACTTTATTGCCTAAAGTCTTAACTCGAATAATCGAAGGACTCCAGGACCTTGATGACGATGTTAGAGCTGTTGCTGCAGCATCCCTGGTTCCTGTAGTAGAAAGCCTTGTCTACCTTCAGACACAAAAGGTACCCTCCATTATAAATACCTTATGGGATTCTCTTCTGGAATTAGATGATCTAACAGCTTCAACAAATAGTATTATGACTCTCCTTTCATCCATGTTAACTTACCCTCAGGTCCAGCAATGCAGTATTCAACAGTCACTCACAGTTTTAGTCCCACGTGTCTGGCCATTTTTGCATCACACTATATCTTCAGTTCGAAGAGCAGCATTGGAAACTCTCTTTACATTATTATCAACACAGGACGAGAACTCTTCATCTTGGCTCATACCTATCCTGTCGGATATGCTGAGACACATTTTTCAGTTTTGTGTTTTAGAAAGCAGCCAGGAAATTCTGGATCTTATTCACAAGGTTTGGATGGAGCTTTTGAGCAAGGCGTCAGTTCAGTATTTGGTAGCAGCTGCATGCCCATGGATGAGTGCTTGGCTTTGCCTAATGATGCAGCCTTCTCACTTACCTATTGATTTAAATATGCTGCTGGAAGTAAAAGCTAGAGCCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCTAATGATGCAGCCTTCT
59.79
AAAGTGATGCCCCATGCTGT
60.25
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGATGAGTGCTTGGCTTTGC
60.11
CCCCATGCTGTAACAATCTGC
59.59
179
PCR template sequence:
TGCATGCCCATGGATGAGTGCTTGGCTTTGCCTAATGATGCAGCCTTCTCACTTACCTATTGATTTAAATATGCTGCTGGAAGTAAAAGCTAGAGCCAAGAACCTACAGAGAGTTCTATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
20
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(2), mmu-miR-322-5p(2), mmu-miR-15b-5p(2), mmu-miR-217-5p(2), mmu-miR-134-5p(1), mmu-miR-15a-5p(1), mmu-miR-93-5p(1), mmu-miR-200b-3p(1), mmu-miR-106b-5p(1), mmu-miR-324-5p(1), mmu-miR-676-3p(1), mmu-miR-127-5p(1), mmu-miR-218-5p(1), mmu-miR-541-5p(1), mmu-miR-499-5p(1), mmu-miR-5101(1), mmu-miR-369-3p(1), mmu-miR-335-3p(1), mmu-miR-1199-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
AGCTAGAGCCAAGAACCTACAGAGAG
siRNA sets
CTCTCTGTAGGTTCTTGGC, TCTCTGTAGGTTCTTGGCT, CTCTGTAGGTTCTTGGCTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTAF1_1649_ORF_1
0.8984
-0.007395023
0.999784799
1680
ORF sequence:
ATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTTGGGGTCTGCTGGTGCAGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGGATGTAATTAATACTTTATTGCCTAAAGTCTTAACTCGAATAATCGAAGGACTCCAGGACCTTGATGACGATGTTAGAGCTGTTGCTGCAGCATCCCTGGTTCCTGTAGTAGAAAGCCTTGTCTACCTTCAGACACAAAAGGTACCCTCCATTATAAATACCTTATGGGATTCTCTTCTGGAATTAGATGATCTAACAGCTTCAACAAATAGTATTATGACTCTCCTTTCATCCATGTTAACTTACCCTCAGGTCCAGCAATGCAGTATTCAACAGTCACTCACAGTTTTAGTCCCACGTGTCTGGCCATTTTTGCATCACACTATATCTTCAGTTCGAAGAGCAGCATTGGAAACTCTCTTTACATTATTATCAACACAGGACGAGAACTCTTCATCTTGGCTCATACCTATCCTGTCGGATATGCTGAGACACATTTTTCAGTTTTGTGTTTTAGAAAGCAGCCAGGAAATTCTGGATCTTATTCACAAGGTTTGGATGGAGCTTTTGAGCAAGGCGTCAGTTCAGTATTTGGTAGCAGCTGCATGCCCATGGATGAGTGCTTGGCTTTGCCTAATGATGCAGCCTTCTCACTTACCTATTGATTTAAATATGCTGCTGGAAGTAAAAGCTAGAGCCAAGAACCTACAGAGAGTTCTATGGAAGATTCATCTACTACAGATCGATTAA
Peptide sequence:
MEDSSTTDRLNFDRFDICRLLQHGASLLGSAGAEFEVQDEKSGEVDPKERIARQRKLLQKKLGLNMGEAIGMSTEELFNDEDLDYTPTSAALVNKQSTLQAAELIDSEFRAGMSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVRQDVINTLLPKVLTRIIEGLQDLDDDVRAVAAASLVPVVESLVYLQTQKVPSIINTLWDSLLELDDLTASTNSIMTLLSSMLTYPQVQQCSIQQSLTVLVPRVWPFLHHTISSVRRAALETLFTLLSTQDENSSSWLIPILSDMLRHIFQFCVLESSQEILDLIHKVWMELLSKASVQYLVAAACPWMSAWLCLMMQPSHLPIDLNMLLEVKARAKNLQRVLWKIHLLQID
IRESfinder result
IRESfinder score
0.680913737
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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