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mmu_circBtaf1_1511
Basic information of circular RNA
PanCircBase ID:
mmu_circBtaf1_1511
mm39_Chr. Position ID:
chr19|36966787|36979939|+
Gene name:
Btaf1
Transcript:
ENSMUST00000099494.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
11
Exon/intron indexes:
23,24,25,26,27,28,29,30,31,32,33
Exon/intron offsets:
0,1335,3048,4882,5203,5411,7142,8118,10704,10929,13026
Genomic length:
13152
Exon/intron sizes in nt:
1,10,20,21,49,15,31,22,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr19:36966429-36966787|chr19:36979939-36980887
Splice length:
1511
Other database IDs:
circBase: hsa_circ_0094440, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_76611
Human Ortholog IDs:
Mature spliced Sequence:
ATGGAAAGTCCCTCCTGGAAAGAGGTGATGTTCCTGCTCAAGAATTGGTGAATTCCCTGCAAGTGTTTGAAATAGCAGCAGCTTCCATGGACTCTGCACTTCATCCCTTGCTGGTACAGCACTTGCCTCATCTTTATATGTGCCTTCAGTACCCCAGCACTGCAGTGAGGCACATGGCGGCTCGTTGCATAGGTGTCATGAGTAAAATAGCTACCATGGAAACAATGAATATTTTTTTGGAGAAGGTACTTCCTTGGTTGGGAGCAATTGATGACAATGTCAAGCAAGAAGGTGCAATTGAAGCACTTGCCTGTGTTATGGAACAATTGGATGTCGGTATTGTTCCATATATTGTCCTTTTAGTTGTTCCTGTATTGGGAAGGATGAGTGATCAGACAGACAGTGTGAGATTCATGGCTACTCAGTGCTTTGCAACACTGATTAGACTGATGCCACTTGAGGCAGGCATTCCAGACCCTCCAAACATGTCAGAAGAATTAATCCAGTTGAAAGCAAAAGAGCGACACTTTTTAGAGCAATTGTTAGATGGGAAGAAGTTAGAAAATTACAAGATCCCAGTGCCAATCAATGCTGAACTCAGGAAATATCAGCAGGATGGTGTGAACTGGTTAGCCTTTCTCAATAAGTATAAGCTTCATGGAATTCTGTGTGATGACATGGGCTTAGGAAAAACATTACAGTCCATCTGCATTCTAGCAGGAGATCACTGTCAGAGGGCCCAGGAATATGCAAGATCGAAATTGGCAGAATGTATGCCACTTCCTTCCTTGGTGGTTTGTCCACCAACATTAACAGGTCATTGGGTAGATGAAGTAGGTAAATTTTGTTCCAGAGAATATCTCAATCCGTTGCATTATACTGGCCCTCCCACTGAAAGGATAAGGTTGCAACATCAAGTAAAAAGGCACAATCTGATAGTAGCCTCATATGATGTTGTGAGGAATGACATAGACTTTTTTAGAAATATTAAATTTAACTACTGTATTCTTGATGAAGGCCATGTCATAAAAAATGGAAAAACCAAGTTGTCCAAAGCAGTAAAGCAGCTGACTGCTAATTACAGGATTATTCTTTCTGGAACACCAATCCAGAACAATGTTTTGGAGTTGTGGTCATTATTTGATTTCCTTATGCCTGGATTTTTGGGTACTGAACGCCAGTTTGCTGCTCGATATGGCAAACCTATATTAGCGAGTAGGGATGCACGAAGCTCCAGTCGAGAACAAGAAGCAGGTGTCCTGGCAATGGACGCTCTGCACCGCCAAGTCCTGCCGTTCCTTCTCAGAAGAATGAAAGAAGATGTTTTGCAGGATCTTCCACCTAAAATTATTCAAGACTATTACTGTACTCTCAGCCCTCTCCAGGTTCAGCTTTATGAAGATTTTGCTAAGTCGCGTGCCAAGTGTGATGTTGATGAGACCGTGTCTTCAGCTGCACTTTCAGAAGAAACTGAAAAACCAAAACTTAAAGCGACAGGTCATGTATTCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAGACCGTGTCTTCAGCTG
59.68
GTGCAGAGTCCATGGAAGCT
60.04
175
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGAGACCGTGTCTTCAGCTG
59.68
AGTGCAGAGTCCATGGAAGC
60.04
176
PCR template sequence:
GTCGCGTGCCAAGTGTGATGTTGATGAGACCGTGTCTTCAGCTGCACTTTCAGAAGAAACTGAAAAACCAAAACTTAAAGCGACAGGTCATGTATTCCAGATGGAAAGTCCCTCCTGGAAAGAGGTGATGTTCCTGCTCAAGAATTGGTGAATTCCCTGCAAGTGTTTGAAATAGCAGCAGCTTCCATGGACTCTGCACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-93-5p(2), mmu-miR-96-5p(2), mmu-miR-32-5p(1), mmu-miR-150-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-320-3p(1), mmu-miR-25-3p(1), mmu-miR-340-5p(1), mmu-miR-690(1), mmu-miR-490-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCATGTATTCCAGATGGAAAGTCCCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTAF1_1511_ORF_1
0.8597
0.019758487
0.999104368
1476
ORF sequence:
ATGGACTCTGCACTTCATCCCTTGCTGGTACAGCACTTGCCTCATCTTTATATGTGCCTTCAGTACCCCAGCACTGCAGTGAGGCACATGGCGGCTCGTTGCATAGGTGTCATGAGTAAAATAGCTACCATGGAAACAATGAATATTTTTTTGGAGAAGGTACTTCCTTGGTTGGGAGCAATTGATGACAATGTCAAGCAAGAAGGTGCAATTGAAGCACTTGCCTGTGTTATGGAACAATTGGATGTCGGTATTGTTCCATATATTGTCCTTTTAGTTGTTCCTGTATTGGGAAGGATGAGTGATCAGACAGACAGTGTGAGATTCATGGCTACTCAGTGCTTTGCAACACTGATTAGACTGATGCCACTTGAGGCAGGCATTCCAGACCCTCCAAACATGTCAGAAGAATTAATCCAGTTGAAAGCAAAAGAGCGACACTTTTTAGAGCAATTGTTAGATGGGAAGAAGTTAGAAAATTACAAGATCCCAGTGCCAATCAATGCTGAACTCAGGAAATATCAGCAGGATGGTGTGAACTGGTTAGCCTTTCTCAATAAGTATAAGCTTCATGGAATTCTGTGTGATGACATGGGCTTAGGAAAAACATTACAGTCCATCTGCATTCTAGCAGGAGATCACTGTCAGAGGGCCCAGGAATATGCAAGATCGAAATTGGCAGAATGTATGCCACTTCCTTCCTTGGTGGTTTGTCCACCAACATTAACAGGTCATTGGGTAGATGAAGTAGGTAAATTTTGTTCCAGAGAATATCTCAATCCGTTGCATTATACTGGCCCTCCCACTGAAAGGATAAGGTTGCAACATCAAGTAAAAAGGCACAATCTGATAGTAGCCTCATATGATGTTGTGAGGAATGACATAGACTTTTTTAGAAATATTAAATTTAACTACTGTATTCTTGATGAAGGCCATGTCATAAAAAATGGAAAAACCAAGTTGTCCAAAGCAGTAAAGCAGCTGACTGCTAATTACAGGATTATTCTTTCTGGAACACCAATCCAGAACAATGTTTTGGAGTTGTGGTCATTATTTGATTTCCTTATGCCTGGATTTTTGGGTACTGAACGCCAGTTTGCTGCTCGATATGGCAAACCTATATTAGCGAGTAGGGATGCACGAAGCTCCAGTCGAGAACAAGAAGCAGGTGTCCTGGCAATGGACGCTCTGCACCGCCAAGTCCTGCCGTTCCTTCTCAGAAGAATGAAAGAAGATGTTTTGCAGGATCTTCCACCTAAAATTATTCAAGACTATTACTGTACTCTCAGCCCTCTCCAGGTTCAGCTTTATGAAGATTTTGCTAAGTCGCGTGCCAAGTGTGATGTTGATGAGACCGTGTCTTCAGCTGCACTTTCAGAAGAAACTGAAAAACCAAAACTTAAAGCGACAGGTCATGTATTCCAGATGGAAAGTCCCTCCTGGAAAGAGGTGATGTTCCTGCTCAAGAATTGGTGA
Peptide sequence:
MDSALHPLLVQHLPHLYMCLQYPSTAVRHMAARCIGVMSKIATMETMNIFLEKVLPWLGAIDDNVKQEGAIEALACVMEQLDVGIVPYIVLLVVPVLGRMSDQTDSVRFMATQCFATLIRLMPLEAGIPDPPNMSEELIQLKAKERHFLEQLLDGKKLENYKIPVPINAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCQRAQEYARSKLAECMPLPSLVVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNLIVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPILASRDARSSSREQEAGVLAMDALHRQVLPFLLRRMKEDVLQDLPPKIIQDYYCTLSPLQVQLYEDFAKSRAKCDVDETVSSAALSEETEKPKLKATGHVFQMESPSWKEVMFLLKNW
IRESfinder result
IRESfinder score
0.545484719
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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