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mmu_circBtaf1_1355
Basic information of circular RNA
PanCircBase ID:
mmu_circBtaf1_1355
mm39_Chr. Position ID:
chr19|36933770|36955819|+
Gene name:
Btaf1
Transcript:
ENSMUST00000099494.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
11
Exon/intron indexes:
5,6,7,8,9,10,11,12,13,14,15
Exon/intron offsets:
0,2002,5268,6060,9247,10121,10305,12685,13543,16527,21944
Genomic length:
22049
Exon/intron sizes in nt:
1,64,13,71,30,69,90,96,00,00,00,00,00,00,000
Flanking exon/introns:
chr19:36928577-36933770|chr19:36955819-36957976
Splice length:
1355
Other database IDs:
circAtlas: mmu-Btaf1_0097
Human Ortholog IDs:
Mature spliced Sequence:
GTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGGATGTAATTAATACTTTATTGCCTAAAGTCTTAACTCGAATAATCGAAGGACTCCAGGACCTTGATGACGATGTTAGAGCTGTTGCTGCAGCATCCCTGGTTCCTGTAGTAGAAAGCCTTGTCTACCTTCAGACACAAAAGGTACCCTCCATTATAAATACCTTATGGGATTCTCTTCTGGAATTAGATGATCTAACAGCTTCAACAAATAGTATTATGACTCTCCTTTCATCCATGTTAACTTACCCTCAGGTCCAGCAATGCAGTATTCAACAGTCACTCACAGTTTTAGTCCCACGTGTCTGGCCATTTTTGCATCACACTATATCTTCAGTTCGAAGAGCAGCATTGGAAACTCTCTTTACATTATTATCAACACAGGACGAGAACTCTTCATCTTGGCTCATACCTATCCTGTCGGATATGCTGAGACACATTTTTCAGTTTTGTGTTTTAGAAAGCAGCCAGGAAATTCTGGATCTTATTCACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTGGCTCATACCTATCCTGTCG
59.37
AATAATTTCCGTTGGCGTGCT
59.18
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTCATACCTATCCTGTCGG
59.45
GTAATAATTTCCGTTGGCGTGC
58.9
140
PCR template sequence:
TTCATCTTGGCTCATACCTATCCTGTCGGATATGCTGAGACACATTTTTCAGTTTTGTGTTTTAGAAAGCAGCCAGGAAATTCTGGATCTTATTCACAAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
19
Interacting miRNAs (#binding sites)
mmu-miR-217-5p(2), mmu-miR-134-5p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-93-5p(1), mmu-miR-200b-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-106b-5p(1), mmu-miR-324-5p(1), mmu-miR-676-3p(1), mmu-miR-127-5p(1), mmu-miR-218-5p(1), mmu-miR-541-5p(1), mmu-miR-499-5p(1), mmu-miR-5101(1), mmu-miR-369-3p(1), mmu-miR-1199-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
TCTTATTCACAAGGTGAAGTGGATCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTAF1_1355_ORF_1
0.947
-0.006580486
0.999621931
1587
ORF sequence:
ATGCAGTATTCAACAGTCACTCACAGTTTTAGTCCCACGTGTCTGGCCATTTTTGCATCACACTATATCTTCAGTTCGAAGAGCAGCATTGGAAACTCTCTTTACATTATTATCAACACAGGACGAGAACTCTTCATCTTGGCTCATACCTATCCTGTCGGATATGCTGAGACACATTTTTCAGTTTTGTGTTTTAGAAAGCAGCCAGGAAATTCTGGATCTTATTCACAAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGGATGTAATTAATACTTTATTGCCTAAAGTCTTAACTCGAATAATCGAAGGACTCCAGGACCTTGATGACGATGTTAGAGCTGTTGCTGCAGCATCCCTGGTTCCTGTAGTAGAAAGCCTTGTCTACCTTCAGACACAAAAGGTACCCTCCATTATAAATACCTTATGGGATTCTCTTCTGGAATTAGATGATCTAACAGCTTCAACAAATAGTATTATGACTCTCCTTTCATCCATGTTAACTTACCCTCAGGTCCAGCAATGCAGTATTCAACAGTCACTCACAGTTTTAGTCCCACGTGTCTGGCCATTTTTGCATCACACTATATCTTCAGTTCGAAGAGCAGCATTGGAAACTCTCTTTACATTATTATCAACACAGGACGAGAACTCTTCATCTTGGCTCATACCTATCCTGTCGGATATGCTGAGACACATTTTTCAGTTTTGTGTTTTAGAAAGCAGCCAGGAAATTCTGGATCTTATTCACAAG
Peptide sequence:
MQYSTVTHSFSPTCLAIFASHYIFSSKSSIGNSLYIIINTGRELFILAHTYPVGYAETHFSVLCFRKQPGNSGSYSQGEVDPKERIARQRKLLQKKLGLNMGEAIGMSTEELFNDEDLDYTPTSAALVNKQSTLQAAELIDSEFRAGMSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVRQDVINTLLPKVLTRIIEGLQDLDDDVRAVAAASLVPVVESLVYLQTQKVPSIINTLWDSLLELDDLTASTNSIMTLLSSMLTYPQVQQCSIQQSLTVLVPRVWPFLHHTISSVRRAALETLFTLLSTQDENSSSWLIPILSDMLRHIFQFCVLESSQEILDLIHK
IRESfinder result
IRESfinder score
0.682218504
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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