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mmu_circBtaf1_1191
Basic information of circular RNA
PanCircBase ID:
mmu_circBtaf1_1191
mm39_Chr. Position ID:
chr19|36935772|36955819|+
Gene name:
Btaf1
Transcript:
ENSMUST00000099494.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
6,7,8,9,10,11,12,13,14,15
Exon/intron offsets:
0,3266,4058,7245,8119,8303,10683,11541,14525,19942
Genomic length:
20047
Exon/intron sizes in nt:
13,71,30,69,90,96,17,70,00,00,00,00,000
Flanking exon/introns:
chr19:36933934-36935772|chr19:36955819-36957976
Splice length:
1191
Other database IDs:
CIRCpedia: MMU_CIRCpedia_38175, circAtlas: mmu-Btaf1_0052
Human Ortholog IDs:
Mature spliced Sequence:
ACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGGATGTAATTAATACTTTATTGCCTAAAGTCTTAACTCGAATAATCGAAGGACTCCAGGACCTTGATGACGATGTTAGAGCTGTTGCTGCAGCATCCCTGGTTCCTGTAGTAGAAAGCCTTGTCTACCTTCAGACACAAAAGGTACCCTCCATTATAAATACCTTATGGGATTCTCTTCTGGAATTAGATGATCTAACAGCTTCAACAAATAGTATTATGACTCTCCTTTCATCCATGTTAACTTACCCTCAGGTCCAGCAATGCAGTATTCAACAGTCACTCACAGTTTTAGTCCCACGTGTCTGGCCATTTTTGCATCACACTATATCTTCAGTTCGAAGAGCAGCATTGGAAACTCTCTTTACATTATTATCAACACAGGACGAGAACTCTTCATCTTGGCTCATACCTATCCTGTCGGATATGCTGAGACACATTTTTCAGTTTTGTGTTTTAGAAAGCAGCCAGGAAATTCTGGATCTTATTCACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTCATACCTATCCTGTCGG
59.45
ATTACTCATTCCTGCTCGGA
56.03
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTCATACCTATCCTGTCGG
59.45
TGTCTATTACTCATTCCTGCTCGG
59.96
151
PCR template sequence:
TTCATCTTGGCTCATACCTATCCTGTCGGATATGCTGAGACACATTTTTCAGTTTTGTGTTTTAGAAAGCAGCCAGGAAATTCTGGATCTTATTCACAAGACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
18
Interacting miRNAs (#binding sites)
mmu-miR-217-5p(2), mmu-miR-134-5p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-93-5p(1), mmu-miR-200b-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-106b-5p(1), mmu-miR-324-5p(1), mmu-miR-676-3p(1), mmu-miR-127-5p(1), mmu-miR-218-5p(1), mmu-miR-541-5p(1), mmu-miR-499-5p(1), mmu-miR-369-3p(1), mmu-miR-1199-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
TCTTATTCACAAGACTCTCCAAGCAG
siRNA sets
CTGCTTGGAGAGTCTTGTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTAF1_1191_ORF_1
0.9668
-0.014889531
0.99999833
2337
ORF sequence:
ATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGGATGTAATTAATACTTTATTGCCTAAAGTCTTAACTCGAATAATCGAAGGACTCCAGGACCTTGATGACGATGTTAGAGCTGTTGCTGCAGCATCCCTGGTTCCTGTAGTAGAAAGCCTTGTCTACCTTCAGACACAAAAGGTACCCTCCATTATAAATACCTTATGGGATTCTCTTCTGGAATTAGATGATCTAACAGCTTCAACAAATAGTATTATGACTCTCCTTTCATCCATGTTAACTTACCCTCAGGTCCAGCAATGCAGTATTCAACAGTCACTCACAGTTTTAGTCCCACGTGTCTGGCCATTTTTGCATCACACTATATCTTCAGTTCGAAGAGCAGCATTGGAAACTCTCTTTACATTATTATCAACACAGGACGAGAACTCTTCATCTTGGCTCATACCTATCCTGTCGGATATGCTGAGACACATTTTTCAGTTTTGTGTTTTAGAAAGCAGCCAGGAAATTCTGGATCTTATTCACAAGACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGGATGTAATTAATACTTTATTGCCTAAAGTCTTAACTCGAATAATCGAAGGACTCCAGGACCTTGATGACGATGTTAGAGCTGTTGCTGCAGCATCCCTGGTTCCTGTAGTAGAAAGCCTTGTCTACCTTCAGACACAAAAGGTACCCTCCATTATAAATACCTTATGGGATTCTCTTCTGGAATTAGATGATCTAACAGCTTCAACAAATAGTATTATGACTCTCCTTTCATCCATGTTAACTTACCCTCAGGTCCAGCAATGCAGTATTCAACAGTCACTCACAGTTTTAGTCCCACGTGTCTGGCCATTTTTGCATCACACTATATCTTCAGTTCGAAGAGCAGCATTGGAAACTCTCTTTACATTATTATCAACACAGGACGAGAACTCTTCATCTTGGCTCATACCTATCCTGTCGGATATGCTGAGACACATTTTTCAGTTTTGTGTTTTAGAAAGCAGCCAGGAAATTCTGGATCTTATTCACAAG
Peptide sequence:
MSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVRQDVINTLLPKVLTRIIEGLQDLDDDVRAVAAASLVPVVESLVYLQTQKVPSIINTLWDSLLELDDLTASTNSIMTLLSSMLTYPQVQQCSIQQSLTVLVPRVWPFLHHTISSVRRAALETLFTLLSTQDENSSSWLIPILSDMLRHIFQFCVLESSQEILDLIHKTLQAAELIDSEFRAGMSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVRQDVINTLLPKVLTRIIEGLQDLDDDVRAVAAASLVPVVESLVYLQTQKVPSIINTLWDSLLELDDLTASTNSIMTLLSSMLTYPQVQQCSIQQSLTVLVPRVWPFLHHTISSVRRAALETLFTLLSTQDENSSSWLIPILSDMLRHIFQFCVLESSQEILDLIHK
IRESfinder result
IRESfinder score
0.694942917
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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