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mmu_circBtaf1_1122
Basic information of circular RNA
PanCircBase ID:
mmu_circBtaf1_1122
mm39_Chr. Position ID:
chr19|36926467|36944252|+
Gene name:
Btaf1
Transcript:
ENSMUST00000099494.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
3,4,5,6,7,8,9,10,11
Exon/intron offsets:
0,1966,7303,9305,12571,13363,16550,17424,17608
Genomic length:
17785
Exon/intron sizes in nt:
1,15,14,41,64,13,71,30,00,00,00,000
Flanking exon/introns:
chr19:36922735-36926467|chr19:36944252-36946455
Splice length:
1122
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GTATTAATATATTTAAGGAGTACAAATTGGGATACCCGGATTGCAGCAGGACAAGCTGTTGAAGCTATAGTGAAAAACGTGCCTGAGTGGAATCCAGTGCCAAGAACCAAACAAGAACCTACAGAGAGTTCTATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTTGGGGTCTGCTGGTGCAGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGTGGATGTGCTGCTGAA
59.89
GCTTCAACAGCTTGTCCTGC
60.04
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGTGGATGTGCTGCTGAA
59.89
CTGCAATCCGGGTATCCCAA
59.82
142
PCR template sequence:
TAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGGTATTAATATATTTAAGGAGTACAAATTGGGATACCCGGATTGCAGCAGGACAAGCTGTTGAAGCTATAGTGAAAAACGTGCCTGAGTGGAATCCAGTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-5101(2), mmu-miR-335-3p(2), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-93-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-106b-5p(1), mmu-miR-217-5p(1), mmu-miR-324-5p(1), mmu-miR-218-5p(1), mmu-miR-541-5p(1), mmu-miR-1199-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCAGTTCGTCAGGTATTAATATATT
siRNA sets
AATATATTAATACCTGACG, TATTAATACCTGACGAACT, ATATATTAATACCTGACGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTAF1_1122_ORF_1
1.0641
0.048418447
0.999994562
2112
ORF sequence:
ATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTTGGGGTCTGCTGGTGCAGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGGTATTAATATATTTAAGGAGTACAAATTGGGATACCCGGATTGCAGCAGGACAAGCTGTTGAAGCTATAGTGAAAAACGTGCCTGAGTGGAATCCAGTGCCAAGAACCAAACAAGAACCTACAGAGAGTTCTATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTTGGGGTCTGCTGGTGCAGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAG
Peptide sequence:
MEDSSTTDRLNFDRFDICRLLQHGASLLGSAGAEFEVQDEKSGEVDPKERIARQRKLLQKKLGLNMGEAIGMSTEELFNDEDLDYTPTSAALVNKQSTLQAAELIDSEFRAGMSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVRQVLIYLRSTNWDTRIAAGQAVEAIVKNVPEWNPVPRTKQEPTESSMEDSSTTDRLNFDRFDICRLLQHGASLLGSAGAEFEVQDEKSGEVDPKERIARQRKLLQKKLGLNMGEAIGMSTEELFNDEDLDYTPTSAALVNKQSTLQAAELIDSEFRAGMSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVRQ
IRESfinder result
IRESfinder score
0.608361505
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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