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mmu_circBrwd1_665
Basic information of circular RNA
PanCircBase ID:
mmu_circBrwd1_665
mm39_Chr. Position ID:
chr16|95818780|95825076|-
Gene name:
Brwd1
Transcript:
ENSMUST00000099502.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
35,34,33,32,31,30
Exon/intron offsets:
0,1838,2098,3635,5822,6140
Genomic length:
6296
Exon/intron sizes in nt:
144,42,76,126,121,156
Flanking exon/introns:
chr16:95817460-95818780|chr16:95825076-95828544
Splice length:
665
Other database IDs:
CIRCpedia: MMU_CIRCpedia_35258, circAtlas: mmu-Brwd1_0029
CIRCpedia: HSA_CIRCpedia_356288
Human Ortholog IDs:
Mature spliced Sequence:
TTGATCCTCCAGAAGAATTGGGAGCAAGTATCTCTGTCACTTCAGATGAGCTAGAGAAATTGCTCTACAAACCACAAGAAGGAGAATGGGGCCAGAGGTCAAGAGATGAGGAGTGTGATCGGATCATCAGTGGCATAGACCAGCTTTTAAACCTCGATATAGCAGCAGCGTTTGCAGGCCCGGTGGATCTGTGCACATATCCCAAGTACTGCACAGTAGTGGCTTATCCAACTGATCTCTACACAATTCGAATGAGGCTTGTTAATCGATTTTACAGGCGGTTATCTGCATTAATTTGGGAAGTCCGATATATAGAGCATAATGCAAGAACGTTTAATGAACCGGAAAGTGTAATTGCAAGATCAGCTAAAAAAATAACTGACCAGCTTCTGAAGTTTATCAAGAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACGACAGTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAGGTCCACAACTGGAAAAGGAGGAGTAGAGCCAGTGGCTGTATTGAGAGTGACTGGAGGAGGCAGTGCAAGGCCCTGCTTATCCTCATCTTCCAGTGTGAGGACTCGGAGCCCTTTAGACAGCCCGTGGACTTGGATGAGTACCCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAGGACTCGGAGCCCTTTA
59.96
CTCTGGCCCCATTCTCCTTC
59.82
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGAGGACTCGGAGCCCTTTA
59.96
TCTGGCCCCATTCTCCTTCT
60.25
145
PCR template sequence:
GAGTGACTGGAGGAGGCAGTGCAAGGCCCTGCTTATCCTCATCTTCCAGTGTGAGGACTCGGAGCCCTTTAGACAGCCCGTGGACTTGGATGAGTACCCATTGATCCTCCAGAAGAATTGGGAGCAAGTATCTCTGTCACTTCAGATGAGCTAGAGAAATTGCTCTACAAACCACAAGAAGGAGAATGGGGCCAGAGGTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-150-5p(1), mmu-miR-320-3p(1), mmu-miR-149-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGATGAGTACCCATTGATCCTCCAGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBRWD1_665_ORF_1
0.8454
0.096516219
0.517439692
468
ORF sequence:
ATGAGGCTTGTTAATCGATTTTACAGGCGGTTATCTGCATTAATTTGGGAAGTCCGATATATAGAGCATAATGCAAGAACGTTTAATGAACCGGAAAGTGTAATTGCAAGATCAGCTAAAAAAATAACTGACCAGCTTCTGAAGTTTATCAAGAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACGACAGTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAGGTCCACAACTGGAAAAGGAGGAGTAGAGCCAGTGGCTGTATTGAGAGTGACTGGAGGAGGCAGTGCAAGGCCCTGCTTATCCTCATCTTCCAGTGTGAGGACTCGGAGCCCTTTAGACAGCCCGTGGACTTGGATGAGTACCCATTGATCCTCCAGAAGAATTGGGAGCAAGTATCTCTGTCACTTCAGATGAGCTAG
Peptide sequence:
MRLVNRFYRRLSALIWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQDCTNISELCNTSDNDEDDSADDLDDSDLPKTSSGRRKVHNWKRRSRASGCIESDWRRQCKALLILIFQCEDSEPFRQPVDLDEYPLILQKNWEQVSLSLQMS
IRESfinder result
IRESfinder score
0.62473616
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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