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mmu_circBrwd1_2655
Basic information of circular RNA
PanCircBase ID:
mmu_circBrwd1_2655
mm39_Chr. Position ID:
chr16|95818780|95853859|-
Gene name:
Brwd1
Transcript:
ENSMUST00000099502.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
21
Exon/intron indexes:
35,34,33,32,31,30,29,28,27,26,25,24,23,22,21,20,19,18,17,16,15
Exon/intron offsets:
0,1838,2098,3635,5822,6140,9764,10596,13054,13946,16004,16187,18281,20310,23671,25420,26883,28764,29354,29738,34944
Genomic length:
35079
Exon/intron sizes in nt:
14,44,27,61,26,12,11,50,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr16:95817460-95818780|chr16:95853859-95855031
Splice length:
2655
Other database IDs:
circAtlas: mmu-Brwd1_0117
Human Ortholog IDs:
Mature spliced Sequence:
GGACATGCTGATGAAGTGTTTGTTTTGGAGACACATCCCTTTGATTCCAGAATTATGTTATCTGCAGGACACGATGGCAGCATATTTATATGGGATATTACAAAGGGCATCAAGATGAAGCATTACTTTAATATGATCGAAGGACAAGGACATGGAGCTGTGTTTGACTGCAAGTTTTCACAGGATGGACAACATTTTGCCTGTACAGATTCTCACGGGCATCTTCTGATATTTGGCTTTGGATGCAGCAAGCCATATGAAAAGATTCCTGATCAGATGTTTTTTCATACTGACTACCGACCACTGATAAGAGATTCCAATAACTATGTCTTGGATGAGCAGACACAGCAGGCACCTCATCTTATGCCTCCACCATTCTTGGTGGATGTGGACGGAAACCCTCATCCAACCAAGTTCCAGAGATTAGTACCAGGTCGAGAAAACTCAGCAGATGAACATTTGGTTCCACAGCTAGGCTATGTGGCCACAAGTGATGGGGAGGTGATTGAACAGATTATAAGCCTGCATACCAACGACAGTGGAGATGCAAGCCCAGAGTCCAGCGTCTTGGATGGCATGATTAGGCAGCTGCAGCAGCAGCAAGACCAGAGACTGGGAGTGGATCAGGATGGGACTGCAAATGGACTTCCCAGTGGGGAAGGGACACCCCGAAGAGGGAGTTTTAGAAGACTGAGTTTAGACATTCAGTCTCCTCCAAACATCGGTCTCCGTCGGAGTGGGCAGGTTGAAGGTGTTCGTCAGATGCATCAGAACGCTCCTCGGAGTCAGATTGCTACAGAGCGTGACCTCCAGGCTTGGAAGCGGAGAGTAGTTGTTCCAGAGGCACCGCCTGGCATGTTCAGGCGGTTGGAAGACATTCGCATAGAGAGAGGCGAGGAGGAGAGGAACCTGTATGTAATAGGGAGAAAGAAGAAGACTTTCCAGGTCCCACAAAAGTCCAATTCGATGGTTTTGGTGTCCCAGTCTAGACAACGGACGTGTAGGCGTAAATATGCAAATTATAGTAGAAGAAACGCTGACCAGTGTGAATTATCTTCTGGAAATGAATCTTCAGGCTCTGTTAGACATGAGACATCTTATGATCAGAGTGAAGGTTCTTGTTCTTCAGAAGATGACGAGTGGAGAAATGACAGAAGAAGTGGTAGTGACAGCGACAGCTCAAGTGACTCTTCATCTCAGTATTCTGATTGGACAGCTGACACGGGGATCAACTTGCAGCCTCCTCTCAGGATGTCATGTCGTCGTCGAGTAACTCGATTCTGCAGTACTTCAGAGGACGAAATGTCCATGGAGAACATATCACCTCCTAAACGAAGGCGGAAAAGAAGGAAAGAAAGCAAGCCTAAGAGGGAGAGTTTACGAAGGATGACTCCAGCAGAACTTGCAAATATGGAACACCTATATGAGTTTCACCCTCCTGTGTGGATAACGGACACCACACTTCGGAAGTCTCCCTTTGTTCCTCAGATGGGTGATGAGGTGATGTACTTCCGTCAGGGTCATGAAGCTTATATTGAAGCTGTCAGAAGAAATAACATTTATGAACTAAATCCTCACAAGGAGCCATGGAGGAAAATGGATCTTAGGGATCAAGAACTGGTCAAAATTGTTGGACTAAGGTATGAGGTTGGACCACCTACACTTTGTTGCCTCAAACTAGCATTTATAGATCCAGCGACTGGCAGACTGACGGATAAATCTTTCTCTATTAGATATCATGACATGCCAGATGTTATTGATTTTCTTGTGTTGCGCCAATTTTATGATGAAGCAAGACAGAGAAACTGGCAGCCTTGTGACAGATTTCGATCTATAATTGATGATGCTTGGTGGTTTGGAACCGTGTTGAGTCAAGAACCGTATCAACCCCAGTATCCTGACAGTCACTTCCAGTGTTACATTGTTAGGTGGGACAACACCGAGACTGAAAAGCTTAGCCCTTGGGACATGGAACCGATTCCTGACAATGTTGATCCTCCAGAAGAATTGGGAGCAAGTATCTCTGTCACTTCAGATGAGCTAGAGAAATTGCTCTACAAACCACAAGAAGGAGAATGGGGCCAGAGGTCAAGAGATGAGGAGTGTGATCGGATCATCAGTGGCATAGACCAGCTTTTAAACCTCGATATAGCAGCAGCGTTTGCAGGCCCGGTGGATCTGTGCACATATCCCAAGTACTGCACAGTAGTGGCTTATCCAACTGATCTCTACACAATTCGAATGAGGCTTGTTAATCGATTTTACAGGCGGTTATCTGCATTAATTTGGGAAGTCCGATATATAGAGCATAATGCAAGAACGTTTAATGAACCGGAAAGTGTAATTGCAAGATCAGCTAAAAAAATAACTGACCAGCTTCTGAAGTTTATCAAGAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACGACAGTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAGGTCCACAACTGGAAAAGGAGGAGTAGAGCCAGTGGCTGTATTGAGAGTGACTGGAGGAGGCAGTGCAAGGCCCTGCTTATCCTCATCTTCCAGTGTGAGGACTCGGAGCCCTTTAGACAGCCCGTGGACTTGGATGAGTACCCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTCCAGTGTGAGGACTCGGA
60.18
TATGCTGCCATCGTGTCCTG
60.18
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTTCCAGTGTGAGGACTCGG
59.76
TATGCTGCCATCGTGTCCTG
60.18
142
PCR template sequence:
GAGTGACTGGAGGAGGCAGTGCAAGGCCCTGCTTATCCTCATCTTCCAGTGTGAGGACTCGGAGCCCTTTAGACAGCCCGTGGACTTGGATGAGTACCCAGGACATGCTGATGAAGTGTTTGTTTTGGAGACACATCCCTTTGATTCCAGAATTATGTTATCTGCAGGACACGATGGCAGCATATTTATATGGGATATTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
16
Interacting miRNAs (#binding sites)
mmu-miR-155-5p(1), mmu-miR-150-5p(1), mmu-miR-221-3p(1), mmu-miR-135a-5p(1), mmu-miR-320-3p(1), mmu-miR-326-3p(1), mmu-miR-143-3p(1), mmu-miR-149-5p(1), mmu-miR-125a-5p(1), mmu-miR-411-3p(1), mmu-miR-383-5p(1), mmu-miR-342-5p(1), mmu-miR-190b-5p(1), mmu-miR-207(1), mmu-miR-673-5p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGATGAGTACCCAGGACATGCTGATG
siRNA sets
CATGTCCTGGGTACTCATC, ATCAGCATGTCCTGGGTAC, CAGCATGTCCTGGGTACTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBRWD1_2655_ORF_1
0.8626
0.135705729
1
5256
ORF sequence:
ATGTTATCTGCAGGACACGATGGCAGCATATTTATATGGGATATTACAAAGGGCATCAAGATGAAGCATTACTTTAATATGATCGAAGGACAAGGACATGGAGCTGTGTTTGACTGCAAGTTTTCACAGGATGGACAACATTTTGCCTGTACAGATTCTCACGGGCATCTTCTGATATTTGGCTTTGGATGCAGCAAGCCATATGAAAAGATTCCTGATCAGATGTTTTTTCATACTGACTACCGACCACTGATAAGAGATTCCAATAACTATGTCTTGGATGAGCAGACACAGCAGGCACCTCATCTTATGCCTCCACCATTCTTGGTGGATGTGGACGGAAACCCTCATCCAACCAAGTTCCAGAGATTAGTACCAGGTCGAGAAAACTCAGCAGATGAACATTTGGTTCCACAGCTAGGCTATGTGGCCACAAGTGATGGGGAGGTGATTGAACAGATTATAAGCCTGCATACCAACGACAGTGGAGATGCAAGCCCAGAGTCCAGCGTCTTGGATGGCATGATTAGGCAGCTGCAGCAGCAGCAAGACCAGAGACTGGGAGTGGATCAGGATGGGACTGCAAATGGACTTCCCAGTGGGGAAGGGACACCCCGAAGAGGGAGTTTTAGAAGACTGAGTTTAGACATTCAGTCTCCTCCAAACATCGGTCTCCGTCGGAGTGGGCAGGTTGAAGGTGTTCGTCAGATGCATCAGAACGCTCCTCGGAGTCAGATTGCTACAGAGCGTGACCTCCAGGCTTGGAAGCGGAGAGTAGTTGTTCCAGAGGCACCGCCTGGCATGTTCAGGCGGTTGGAAGACATTCGCATAGAGAGAGGCGAGGAGGAGAGGAACCTGTATGTAATAGGGAGAAAGAAGAAGACTTTCCAGGTCCCACAAAAGTCCAATTCGATGGTTTTGGTGTCCCAGTCTAGACAACGGACGTGTAGGCGTAAATATGCAAATTATAGTAGAAGAAACGCTGACCAGTGTGAATTATCTTCTGGAAATGAATCTTCAGGCTCTGTTAGACATGAGACATCTTATGATCAGAGTGAAGGTTCTTGTTCTTCAGAAGATGACGAGTGGAGAAATGACAGAAGAAGTGGTAGTGACAGCGACAGCTCAAGTGACTCTTCATCTCAGTATTCTGATTGGACAGCTGACACGGGGATCAACTTGCAGCCTCCTCTCAGGATGTCATGTCGTCGTCGAGTAACTCGATTCTGCAGTACTTCAGAGGACGAAATGTCCATGGAGAACATATCACCTCCTAAACGAAGGCGGAAAAGAAGGAAAGAAAGCAAGCCTAAGAGGGAGAGTTTACGAAGGATGACTCCAGCAGAACTTGCAAATATGGAACACCTATATGAGTTTCACCCTCCTGTGTGGATAACGGACACCACACTTCGGAAGTCTCCCTTTGTTCCTCAGATGGGTGATGAGGTGATGTACTTCCGTCAGGGTCATGAAGCTTATATTGAAGCTGTCAGAAGAAATAACATTTATGAACTAAATCCTCACAAGGAGCCATGGAGGAAAATGGATCTTAGGGATCAAGAACTGGTCAAAATTGTTGGACTAAGGTATGAGGTTGGACCACCTACACTTTGTTGCCTCAAACTAGCATTTATAGATCCAGCGACTGGCAGACTGACGGATAAATCTTTCTCTATTAGATATCATGACATGCCAGATGTTATTGATTTTCTTGTGTTGCGCCAATTTTATGATGAAGCAAGACAGAGAAACTGGCAGCCTTGTGACAGATTTCGATCTATAATTGATGATGCTTGGTGGTTTGGAACCGTGTTGAGTCAAGAACCGTATCAACCCCAGTATCCTGACAGTCACTTCCAGTGTTACATTGTTAGGTGGGACAACACCGAGACTGAAAAGCTTAGCCCTTGGGACATGGAACCGATTCCTGACAATGTTGATCCTCCAGAAGAATTGGGAGCAAGTATCTCTGTCACTTCAGATGAGCTAGAGAAATTGCTCTACAAACCACAAGAAGGAGAATGGGGCCAGAGGTCAAGAGATGAGGAGTGTGATCGGATCATCAGTGGCATAGACCAGCTTTTAAACCTCGATATAGCAGCAGCGTTTGCAGGCCCGGTGGATCTGTGCACATATCCCAAGTACTGCACAGTAGTGGCTTATCCAACTGATCTCTACACAATTCGAATGAGGCTTGTTAATCGATTTTACAGGCGGTTATCTGCATTAATTTGGGAAGTCCGATATATAGAGCATAATGCAAGAACGTTTAATGAACCGGAAAGTGTAATTGCAAGATCAGCTAAAAAAATAACTGACCAGCTTCTGAAGTTTATCAAGAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACGACAGTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAGGTCCACAACTGGAAAAGGAGGAGTAGAGCCAGTGGCTGTATTGAGAGTGACTGGAGGAGGCAGTGCAAGGCCCTGCTTATCCTCATCTTCCAGTGTGAGGACTCGGAGCCCTTTAGACAGCCCGTGGACTTGGATGAGTACCCAGGACATGCTGATGAAGTGTTTGTTTTGGAGACACATCCCTTTGATTCCAGAATTATGTTATCTGCAGGACACGATGGCAGCATATTTATATGGGATATTACAAAGGGCATCAAGATGAAGCATTACTTTAATATGATCGAAGGACAAGGACATGGAGCTGTGTTTGACTGCAAGTTTTCACAGGATGGACAACATTTTGCCTGTACAGATTCTCACGGGCATCTTCTGATATTTGGCTTTGGATGCAGCAAGCCATATGAAAAGATTCCTGATCAGATGTTTTTTCATACTGACTACCGACCACTGATAAGAGATTCCAATAACTATGTCTTGGATGAGCAGACACAGCAGGCACCTCATCTTATGCCTCCACCATTCTTGGTGGATGTGGACGGAAACCCTCATCCAACCAAGTTCCAGAGATTAGTACCAGGTCGAGAAAACTCAGCAGATGAACATTTGGTTCCACAGCTAGGCTATGTGGCCACAAGTGATGGGGAGGTGATTGAACAGATTATAAGCCTGCATACCAACGACAGTGGAGATGCAAGCCCAGAGTCCAGCGTCTTGGATGGCATGATTAGGCAGCTGCAGCAGCAGCAAGACCAGAGACTGGGAGTGGATCAGGATGGGACTGCAAATGGACTTCCCAGTGGGGAAGGGACACCCCGAAGAGGGAGTTTTAGAAGACTGAGTTTAGACATTCAGTCTCCTCCAAACATCGGTCTCCGTCGGAGTGGGCAGGTTGAAGGTGTTCGTCAGATGCATCAGAACGCTCCTCGGAGTCAGATTGCTACAGAGCGTGACCTCCAGGCTTGGAAGCGGAGAGTAGTTGTTCCAGAGGCACCGCCTGGCATGTTCAGGCGGTTGGAAGACATTCGCATAGAGAGAGGCGAGGAGGAGAGGAACCTGTATGTAATAGGGAGAAAGAAGAAGACTTTCCAGGTCCCACAAAAGTCCAATTCGATGGTTTTGGTGTCCCAGTCTAGACAACGGACGTGTAGGCGTAAATATGCAAATTATAGTAGAAGAAACGCTGACCAGTGTGAATTATCTTCTGGAAATGAATCTTCAGGCTCTGTTAGACATGAGACATCTTATGATCAGAGTGAAGGTTCTTGTTCTTCAGAAGATGACGAGTGGAGAAATGACAGAAGAAGTGGTAGTGACAGCGACAGCTCAAGTGACTCTTCATCTCAGTATTCTGATTGGACAGCTGACACGGGGATCAACTTGCAGCCTCCTCTCAGGATGTCATGTCGTCGTCGAGTAACTCGATTCTGCAGTACTTCAGAGGACGAAATGTCCATGGAGAACATATCACCTCCTAAACGAAGGCGGAAAAGAAGGAAAGAAAGCAAGCCTAAGAGGGAGAGTTTACGAAGGATGACTCCAGCAGAACTTGCAAATATGGAACACCTATATGAGTTTCACCCTCCTGTGTGGATAACGGACACCACACTTCGGAAGTCTCCCTTTGTTCCTCAGATGGGTGATGAGGTGATGTACTTCCGTCAGGGTCATGAAGCTTATATTGAAGCTGTCAGAAGAAATAACATTTATGAACTAAATCCTCACAAGGAGCCATGGAGGAAAATGGATCTTAGGGATCAAGAACTGGTCAAAATTGTTGGACTAAGGTATGAGGTTGGACCACCTACACTTTGTTGCCTCAAACTAGCATTTATAGATCCAGCGACTGGCAGACTGACGGATAAATCTTTCTCTATTAGATATCATGACATGCCAGATGTTATTGATTTTCTTGTGTTGCGCCAATTTTATGATGAAGCAAGACAGAGAAACTGGCAGCCTTGTGACAGATTTCGATCTATAATTGATGATGCTTGGTGGTTTGGAACCGTGTTGAGTCAAGAACCGTATCAACCCCAGTATCCTGACAGTCACTTCCAGTGTTACATTGTTAGGTGGGACAACACCGAGACTGAAAAGCTTAGCCCTTGGGACATGGAACCGATTCCTGACAATGTTGATCCTCCAGAAGAATTGGGAGCAAGTATCTCTGTCACTTCAGATGAGCTAGAGAAATTGCTCTACAAACCACAAGAAGGAGAATGGGGCCAGAGGTCAAGAGATGAGGAGTGTGATCGGATCATCAGTGGCATAGACCAGCTTTTAAACCTCGATATAGCAGCAGCGTTTGCAGGCCCGGTGGATCTGTGCACATATCCCAAGTACTGCACAGTAGTGGCTTATCCAACTGATCTCTACACAATTCGAATGAGGCTTGTTAATCGATTTTACAGGCGGTTATCTGCATTAATTTGGGAAGTCCGATATATAGAGCATAATGCAAGAACGTTTAATGAACCGGAAAGTGTAATTGCAAGATCAGCTAAAAAAATAACTGACCAGCTTCTGAAGTTTATCAAGAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACGACAGTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAGGTCCACAACTGGAAAAGGAGGAGTAGAGCCAGTGGCTGTATTGAGAGTGACTGGAGGAGGCAGTGCAAGGCCCTGCTTATCCTCATCTTCCAGTGTGAGGACTCGGAGCCCTTTAGACAGCCCGTGGACTTGGATGAGTACCCA
Peptide sequence:
MLSAGHDGSIFIWDITKGIKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKFQRLVPGRENSADEHLVPQLGYVATSDGEVIEQIISLHTNDSGDASPESSVLDGMIRQLQQQQDQRLGVDQDGTANGLPSGEGTPRRGSFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEAPPGMFRRLEDIRIERGEEERNLYVIGRKKKTFQVPQKSNSMVLVSQSRQRTCRRKYANYSRRNADQCELSSGNESSGSVRHETSYDQSEGSCSSEDDEWRNDRRSGSDSDSSSDSSSQYSDWTADTGINLQPPLRMSCRRRVTRFCSTSEDEMSMENISPPKRRRKRRKESKPKRESLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVMYFRQGHEAYIEAVRRNNIYELNPHKEPWRKMDLRDQELVKIVGLRYEVGPPTLCCLKLAFIDPATGRLTDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQPCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTETEKLSPWDMEPIPDNVDPPEELGASISVTSDELEKLLYKPQEGEWGQRSRDEECDRIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALIWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQDCTNISELCNTSDNDEDDSADDLDDSDLPKTSSGRRKVHNWKRRSRASGCIESDWRRQCKALLILIFQCEDSEPFRQPVDLDEYPGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGIKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKFQRLVPGRENSADEHLVPQLGYVATSDGEVIEQIISLHTNDSGDASPESSVLDGMIRQLQQQQDQRLGVDQDGTANGLPSGEGTPRRGSFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEAPPGMFRRLEDIRIERGEEERNLYVIGRKKKTFQVPQKSNSMVLVSQSRQRTCRRKYANYSRRNADQCELSSGNESSGSVRHETSYDQSEGSCSSEDDEWRNDRRSGSDSDSSSDSSSQYSDWTADTGINLQPPLRMSCRRRVTRFCSTSEDEMSMENISPPKRRRKRRKESKPKRESLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVMYFRQGHEAYIEAVRRNNIYELNPHKEPWRKMDLRDQELVKIVGLRYEVGPPTLCCLKLAFIDPATGRLTDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQPCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTETEKLSPWDMEPIPDNVDPPEELGASISVTSDELEKLLYKPQEGEWGQRSRDEECDRIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALIWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQDCTNISELCNTSDNDEDDSADDLDDSDLPKTSSGRRKVHNWKRRSRASGCIESDWRRQCKALLILIFQCEDSEPFRQPVDLDEYP
IRESfinder result
IRESfinder score
0.492994625
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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