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mmu_circBrwd1_118
Basic information of circular RNA
PanCircBase ID:
mmu_circBrwd1_118
mm39_Chr. Position ID:
chr16|95820618|95820954|-
Gene name:
Brwd1
Transcript:
ENSMUST00000099502.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
34,33
Exon/intron offsets:
0,260
Genomic length:
336
Exon/intron sizes in nt:
42,76
Flanking exon/introns:
chr16:95818924-95820618|chr16:95820954-95822415
Splice length:
118
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACGACAGTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGTGCTGATGATTTGGATGA
58.04
CGTCCTCATCATTGTCAGAAGTG
59.38
118
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGTGCTGATGATTTGGATGA
58.04
CGTCCTCATCATTGTCAGAAGT
58.15
118
PCR template sequence:
ACAGTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAGGAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
TGGAAGAAGGAAGGAATCAAGATTGT
siRNA sets
ACAATCTTGATTCCTTCCT, ATCTTGATTCCTTCCTTCT, TGATTCCTTCCTTCTTCCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBRWD1_118_ORF_1
1.0801
0.145102042
0.217059501
189
ORF sequence:
ATGATGAGGACGACAGTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAGGAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACGACAGTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAG
Peptide sequence:
MMRTTVLMIWMIVIFLKRLLEEGRNQDCTNISELCNTSDNDEDDSADDLDDSDLPKTSSGRRK
IRESfinder result
IRESfinder score
0.344420243
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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