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mmu_circBrsk2_761
Basic information of circular RNA
PanCircBase ID:
mmu_circBrsk2_761
mm39_Chr. Position ID:
chr7|141545976|141554626|+
Gene name:
Brsk2
Transcript:
ENSMUST00000075528.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
13,14,15,16,17,18,19
Exon/intron offsets:
0,872,1475,4649,6138,6393,8602
Genomic length:
8650
Exon/intron sizes in nt:
61,208,49,124,181,90,48
Flanking exon/introns:
chr7:141542173-141545976|chr7:141554626-141555799
Splice length:
761
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATCTCGATCCATCAGTGGTGCGTCCTCAGGCCTTTCTACAAGTCCACTCAGCAGTCCTCGGGTGACCCCTCACCCCTCACCAAGGGGTAGTCCCCTTCCTACCCCCAAAGGGACGCCTGTCCACACGCCAAAGGAGAGCCCAGCTGGCACACCCAACCCCACACCACCATCCAGCCCTAGTGTTGGAGGAGTGCCCTGGCGGACACGACTGAACTCCATCAAGAACAGCTTCCTGGGCTCACCTCGATTCCACCGCCGGAAACTCCAAGTTCCCACGCCAGAGGAGATGTCCAACCTGACCCCAGAATCCTCTCCAGAGCTGGCCAAGAAATCGTGGTTCGGGAACTTCATCAACCTGGAGAAGGAGGAGCAGATCTTTGTGGTGATCAAGGACAAGCCCCTGAGCTCCATCAAGGCTGACATCGTTCATGCCTTCCTGTCGATCCCCAGCCTCAGCCACAGCGTTATTTCCCAGACAAGCTTCAGGGCTGAATACAAGGCCACAGGGGGCCCAGCAGTGTTCCAGAAGCCGGTCAAGTTCCAGGTGGACATCACCTACACTGAGGGCGGAGAGGCCCAGAAGGAGAATGGCATCTACTCAGTCACATTCACTTTACTCTCAGGCCCCAGTCGCCGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGTTAAGCACCCATGACCAGCCATCAGCCCAGCACCTGTCAGACACCACTAACTGTATGGAAGTGATGACGGGGCGGCTTTCCAAATGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTAAGCACCCATGACCAGCC
60.32
GGACTGCTGAGTGGACTTGT
59.61
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTAAGCACCCATGACCAGCC
60.32
AGGACTGCTGAGTGGACTTG
59.32
146
PCR template sequence:
CAGGCCCAGCTGTTAAGCACCCATGACCAGCCATCAGCCCAGCACCTGTCAGACACCACTAACTGTATGGAAGTGATGACGGGGCGGCTTTCCAAATGTGATCTCGATCCATCAGTGGTGCGTCCTCAGGCCTTTCTACAAGTCCACTCAGCAGTCCTCGGGTGACCCCTCACCCCTCACCAAGGGGTAGTCCCCTTCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-328-3p(1), mmu-miR-326-3p(1), mmu-miR-382-5p(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTTCCAAATGTGATCTCGATCCATC
siRNA sets
GGATCGAGATCACATTTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBRSK2_761_ORF_1
1.297
0.420535172
0.939884375
540
ORF sequence:
ATGTCCAACCTGACCCCAGAATCCTCTCCAGAGCTGGCCAAGAAATCGTGGTTCGGGAACTTCATCAACCTGGAGAAGGAGGAGCAGATCTTTGTGGTGATCAAGGACAAGCCCCTGAGCTCCATCAAGGCTGACATCGTTCATGCCTTCCTGTCGATCCCCAGCCTCAGCCACAGCGTTATTTCCCAGACAAGCTTCAGGGCTGAATACAAGGCCACAGGGGGCCCAGCAGTGTTCCAGAAGCCGGTCAAGTTCCAGGTGGACATCACCTACACTGAGGGCGGAGAGGCCCAGAAGGAGAATGGCATCTACTCAGTCACATTCACTTTACTCTCAGGCCCCAGTCGCCGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGTTAAGCACCCATGACCAGCCATCAGCCCAGCACCTGTCAGACACCACTAACTGTATGGAAGTGATGACGGGGCGGCTTTCCAAATGTGATCTCGATCCATCAGTGGTGCGTCCTCAGGCCTTTCTACAAGTCCACTCAGCAGTCCTCGGGTGA
Peptide sequence:
MSNLTPESSPELAKKSWFGNFINLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDQPSAQHLSDTTNCMEVMTGRLSKCDLDPSVVRPQAFLQVHSAVLG
IRESfinder result
IRESfinder score
0.425372184
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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