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mmu_circBrip1_751
Basic information of circular RNA
PanCircBase ID:
mmu_circBrip1_751
mm39_Chr. Position ID:
chr11|85999592|86030005|-
Gene name:
Brip1
Transcript:
ENSMUST00000044423.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
16,15,14,13,12
Exon/intron offsets:
0,1576,26492,29996,30247
Genomic length:
30413
Exon/intron sizes in nt:
12,21,60,16,21,41,166
Flanking exon/introns:
chr11:85968795-85999592|chr11:86030005-86034082
Splice length:
751
Other database IDs:
CIRCpedia: MMU_CIRCpedia_229852, circAtlas: mmu-Brip1_0007
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_360935
Human Ortholog IDs:
Mature spliced Sequence:
ATTTGCAGATGATTATAAAGTTGCTATTCAACAGACTTACTCCTGGACAAATCAGATTGCTATTTTTGATAAAACTGGAGTCTTGGCTGTACCAAAAAATAAGAAACATTCACGACAGAAAATTGGAGTTAATGCGCTAAACTTTTGGTGTTTAAATCCAGCTGTGGCCTTTTCAGATATTAATGACAAAGTACGGACCATTGTTCTAACATCAGGAACACTCTCACCCCTGAAATCCTTTTCATCTGAGCTTGGTGTCACATTTAGCATCCAACTGGAGGCTAACCATGTCATCAGTAACTCACAGGTTTGGGTTGGTACTGTTGGCTCAGGCCCTAAGGGTCGGAATCTCTGCGCTACCTTTCAGCATACTGAAACATTTGAGTTCCAGGATGAAGTGGGAATGCTTCTGTTATCTGTGTGCCAGACTGTGAGCCAAGGAATTCTGTGCTTTTTGCCATCTTACAAGCTATTAGAGAAATTAAGAGAACGTTGGATTTTCACTGGCCTATGGCATAGTTTGGAGTCAGTGAAGACAGTCATTGCAGAACCCCAAGGAGGAGAAAAAACTGATTTTGATGAATTGCTGCAAGTGTACTATGACGCCATCAAGTTCAAAGGAGAAAAGGATGGAGCCCTTCTCATAGCAGTCTGTCGTGGCAAGGTGAGCGAGGGTCTGGATTTCTCAGATGATAACGCCCGTGCTGTCATAACCGTAGGGATTCCTTTCCCAAATGTGAAGGATCTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCGTGCTGTCATAACCGTA
59.83
TGGTACAGCCAAGACTCCAG
59.02
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCGTGCTGTCATAACCGTA
59.83
GGTACAGCCAAGACTCCAGT
59.02
146
PCR template sequence:
CTGTCGTGGCAAGGTGAGCGAGGGTCTGGATTTCTCAGATGATAACGCCCGTGCTGTCATAACCGTAGGGATTCCTTTCCCAAATGTGAAGGATCTTCAGATTTGCAGATGATTATAAAGTTGCTATTCAACAGACTTACTCCTGGACAAATCAGATTGCTATTTTTGATAAAACTGGAGTCTTGGCTGTACCAAAAAAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-322-5p(1), mmu-miR-23a-3p(1), mmu-miR-539-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAGGATCTTCAGATTTGCAGATGAT
siRNA sets
CTGCAAATCTGAAGATCCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBRIP1_751_ORF_1
0.8028
0.093744001
0.309137586
360
ORF sequence:
ATGCTTCTGTTATCTGTGTGCCAGACTGTGAGCCAAGGAATTCTGTGCTTTTTGCCATCTTACAAGCTATTAGAGAAATTAAGAGAACGTTGGATTTTCACTGGCCTATGGCATAGTTTGGAGTCAGTGAAGACAGTCATTGCAGAACCCCAAGGAGGAGAAAAAACTGATTTTGATGAATTGCTGCAAGTGTACTATGACGCCATCAAGTTCAAAGGAGAAAAGGATGGAGCCCTTCTCATAGCAGTCTGTCGTGGCAAGGTGAGCGAGGGTCTGGATTTCTCAGATGATAACGCCCGTGCTGTCATAACCGTAGGGATTCCTTTCCCAAATGTGAAGGATCTTCAGATTTGCAGATGA
Peptide sequence:
MLLLSVCQTVSQGILCFLPSYKLLEKLRERWIFTGLWHSLESVKTVIAEPQGGEKTDFDELLQVYYDAIKFKGEKDGALLIAVCRGKVSEGLDFSDDNARAVITVGIPFPNVKDLQICR
IRESfinder result
IRESfinder score
0.652996625
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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