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mmu_circBrca2_303
Basic information of circular RNA
PanCircBase ID:
mmu_circBrca2_303
mm39_Chr. Position ID:
chr5|150483892|150484288|+
Gene name:
Brca2
Transcript:
ENSMUST00000044620.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
23,24
Exon/intron offsets:
0,257
Genomic length:
396
Exon/intron sizes in nt:
1,64,139
Flanking exon/introns:
chr5:150483661-150483892|chr5:150484288-150490352
Splice length:
303
Other database IDs:
circAtlas: mmu-Brca2_0012
Human Ortholog IDs:
Mature spliced Sequence:
CTCTGTTGAGTATCTGGCGTCCATCATCAGACTTATCCTCCCTGTTAACAGAAGGAAAGAGATACAGAATCTATCATCTTGCAGTGTCAAAATCGAAGAGTAAATTTGAACGGCCCAGCATCCAGTTAACAGCCACAAAAAGAACTCAGTATCAACAGCTGCCGGTTTCCAGTGAGACTTTACTCCAGGTTTACCAGCCCAGGGAGTCCCTTCACTTCAGCAGACTGTCAGACCCAGCCTTTCAGCCTCCTTGTTCTGAAGTGGATGTCGTAGGAGTTGTAGTTTCTGTTGTAAAACCGATAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCAGACTGTCAGACCCAG
60.04
TCCTTCTGTTAACAGGGAGGA
57.73
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCAGACTGTCAGACCCAG
60.04
TTCCTTCTGTTAACAGGGAGGA
58.41
143
PCR template sequence:
GAGTCCCTTCACTTCAGCAGACTGTCAGACCCAGCCTTTCAGCCTCCTTGTTCTGAAGTGGATGTCGTAGGAGTTGTAGTTTCTGTTGTAAAACCGATAGCTCTGTTGAGTATCTGGCGTCCATCATCAGACTTATCCTCCCTGTTAACAGAAGGAAAGAGATACAGAATCTATCATCTTGCAGTGTCAAAATCGAAGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-490-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTAAAACCGATAGCTCTGTTGAGTAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.820336427
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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