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mmu_circBptf_695
Basic information of circular RNA
PanCircBase ID:
mmu_circBptf_695
mm39_Chr. Position ID:
chr11|106953361|106959225|-
Gene name:
Bptf
Transcript:
ENSMUST00000057892.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
16,15,14,13,12
Exon/intron offsets:
0,3132,3410,4591,5739
Genomic length:
5864
Exon/intron sizes in nt:
2,47,43,15,11,29,125
Flanking exon/introns:
chr11:106952661-106953361|chr11:106959225-106963508
Splice length:
695
Other database IDs:
CIRCpedia: MMU_CIRCpedia_220535
circBase: hsa_circ_0045457
Human Ortholog IDs:
Mature spliced Sequence:
GTATAGACTACAGACGGTAAAGTCCTTGGCTGGAGTGAGCCTGATGTTACGGTTGCTGTGGGCCAGTTTGAGATGGGATGACATGGCAGCCAAGGCTCCTCCAGGAGGAGGGTCTACACGGACAGAAACATCTGAAACTGAAATCACAACCACTGAGATAATTAAGAGGCGTGATGTGGGCCCTTATGGCATTCGTTCTGAGTATTGTATCAGGAAGATCATTTGCCCCATTGGAGTTCCAGAAGCACCAAAAGAAACACCCACACCTCAGCGGAAGGGCCTCCGATCAAGTGCACTTCGGCCAAAGAGACCAGAGACCCCCAAGCAGACGGGCCCTGTTATCATTGAGAGCTGGGTAGCAGAAGAAGAGCTGGAGCTCTGGGAGATCAGGGCTTTTGCTGAGAGAGTGGAGAAAGAAAAGGCTCAAGCAGCAGAGCAGCAGACCAAGAAACGTTTGGAGCAGCAGAAGCCTGCAGTCATCGCAGCGTCCACCACGTCCCCAGCAAACAACACAAGCAGCACTGTCTCTCCAGCCCAGAAGGTGATGGTGGCTCCCTTAAGTGGCTCGGTCACCCCTGGAACAAAGATGGTGCTGGCCACAAAAGTCGGCTCGCCTGCCACAGTCACATTCCAGCAAAACAAGAACTTCCATCAAACTTTTGCCACGTGGGTGAAGCAAGGCCAGTCCAATTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTGCCACAGTCACATTCCA
60.25
GCTGCCATGTCATCCCATCT
60.18
172
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTGCCACAGTCACATTCCA
60.25
CCATCTCAAACTGGCCCACA
60.25
158
PCR template sequence:
GCCACAAAAGTCGGCTCGCCTGCCACAGTCACATTCCAGCAAAACAAGAACTTCCATCAAACTTTTGCCACGTGGGTGAAGCAAGGCCAGTCCAATTCAGGTATAGACTACAGACGGTAAAGTCCTTGGCTGGAGTGAGCCTGATGTTACGGTTGCTGTGGGCCAGTTTGAGATGGGATGACATGGCAGCCAAGGCTCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-1904(2), mmu-miR-122-5p(1), mmu-miR-29b-3p(1), mmu-miR-29a-3p(1), mmu-miR-29c-3p(1), mmu-miR-185-5p(1), mmu-miR-484(1), mmu-miR-193b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGTCCAATTCAGGTATAGACTACAG
siRNA sets
TAGTCTATACCTGAATTGG, CTATACCTGAATTGGACTG, AGTCTATACCTGAATTGGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBPTF_695_ORF_1
0.8626
0.277459694
0.909890055
672
ORF sequence:
ATGTTACGGTTGCTGTGGGCCAGTTTGAGATGGGATGACATGGCAGCCAAGGCTCCTCCAGGAGGAGGGTCTACACGGACAGAAACATCTGAAACTGAAATCACAACCACTGAGATAATTAAGAGGCGTGATGTGGGCCCTTATGGCATTCGTTCTGAGTATTGTATCAGGAAGATCATTTGCCCCATTGGAGTTCCAGAAGCACCAAAAGAAACACCCACACCTCAGCGGAAGGGCCTCCGATCAAGTGCACTTCGGCCAAAGAGACCAGAGACCCCCAAGCAGACGGGCCCTGTTATCATTGAGAGCTGGGTAGCAGAAGAAGAGCTGGAGCTCTGGGAGATCAGGGCTTTTGCTGAGAGAGTGGAGAAAGAAAAGGCTCAAGCAGCAGAGCAGCAGACCAAGAAACGTTTGGAGCAGCAGAAGCCTGCAGTCATCGCAGCGTCCACCACGTCCCCAGCAAACAACACAAGCAGCACTGTCTCTCCAGCCCAGAAGGTGATGGTGGCTCCCTTAAGTGGCTCGGTCACCCCTGGAACAAAGATGGTGCTGGCCACAAAAGTCGGCTCGCCTGCCACAGTCACATTCCAGCAAAACAAGAACTTCCATCAAACTTTTGCCACGTGGGTGAAGCAAGGCCAGTCCAATTCAGGTATAGACTACAGACGGTAA
Peptide sequence:
MLRLLWASLRWDDMAAKAPPGGGSTRTETSETEITTTEIIKRRDVGPYGIRSEYCIRKIICPIGVPEAPKETPTPQRKGLRSSALRPKRPETPKQTGPVIIESWVAEEELELWEIRAFAERVEKEKAQAAEQQTKKRLEQQKPAVIAASTTSPANNTSSTVSPAQKVMVAPLSGSVTPGTKMVLATKVGSPATVTFQQNKNFHQTFATWVKQGQSNSGIDYRR
IRESfinder result
IRESfinder score
0.361945389
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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