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mmu_circBpnt1_552
Basic information of circular RNA
PanCircBase ID:
mmu_circBpnt1_552
mm39_Chr. Position ID:
chr1|185073419|185084576|+
Gene name:
Bpnt1
Transcript:
ENSMUST00000027916.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
3,4,5,6,7
Exon/intron offsets:
0,4092,5452,7521,10959
Genomic length:
11157
Exon/intron sizes in nt:
10,51,08,49,92,198
Flanking exon/introns:
chr1:185070429-185073419|chr1:185084576-185086153
Splice length:
552
Other database IDs:
circBase: mmu_circ_0008538
Human Ortholog IDs:
Mature spliced Sequence:
ACCTCGGCCACCGACCTGCAGACCAAAGCCGACCGCTTGGTGCAGATGAGCATATGCTCTTCCCTGGCCCGGAAGTTCCCGAAGCTGACCATCATAGGGGAAGAGGACCTGCCTCCTGGGGAAGTGGATCAAGAGCTGATTGAAGACGGGCAGTGGGAGGAGATCCTGAAGCAGCCGTGCCCCTCGCAGTACAGTGCTATCAAGGAGGAAGACCTTGTGGTTTGGGTTGACCCCTTAGATGGCACCAAGGAGTACACTGAAGGTCTTCTTGACAATGTAACAGTTCTCATTGGGATTGCTTATGAAGGAAAGGCCATCGCAGGCATCATCAACCAGCCGTATTACAACTACCAGGCAGGACCCGACGCGGCGCTGGGCAGGACCATCTGGGGAGTTCTGGGTTTGGGTGCTTTTGGGTTTCAGCTGAAGGAAGCCCCTGCCGGGAAGCACATTATCACAACCACCAGATCCCATAGCAACCAGCTGGTCACAGACTGCATTTCCGCCATGAACCCCGACACCGTGCTGCGAGTGGGAGGAGCAGGAAACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGACTGCATTTCCGCCATGA
60.04
TGGTCAGCTTCGGGAACTTC
59.97
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGACTGCATTTCCGCCATGA
60.04
TGATGGTCAGCTTCGGGAAC
60.04
155
PCR template sequence:
TATCACAACCACCAGATCCCATAGCAACCAGCTGGTCACAGACTGCATTTCCGCCATGAACCCCGACACCGTGCTGCGAGTGGGAGGAGCAGGAAACAAGACCTCGGCCACCGACCTGCAGACCAAAGCCGACCGCTTGGTGCAGATGAGCATATGCTCTTCCCTGGCCCGGAAGTTCCCGAAGCTGACCATCATAGGGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-135a-5p(1), mmu-miR-146a-5p(1), mmu-miR-216a-5p(1), mmu-miR-1198-5p(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGCAGGAAACAAGACCTCGGCCACCG
siRNA sets
TGGCCGAGGTCTTGTTTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBPNT1_552_ORF_1
1.2541
0.418729253
0.998369706
1059
ORF sequence:
ATGAGCATATGCTCTTCCCTGGCCCGGAAGTTCCCGAAGCTGACCATCATAGGGGAAGAGGACCTGCCTCCTGGGGAAGTGGATCAAGAGCTGATTGAAGACGGGCAGTGGGAGGAGATCCTGAAGCAGCCGTGCCCCTCGCAGTACAGTGCTATCAAGGAGGAAGACCTTGTGGTTTGGGTTGACCCCTTAGATGGCACCAAGGAGTACACTGAAGGTCTTCTTGACAATGTAACAGTTCTCATTGGGATTGCTTATGAAGGAAAGGCCATCGCAGGCATCATCAACCAGCCGTATTACAACTACCAGGCAGGACCCGACGCGGCGCTGGGCAGGACCATCTGGGGAGTTCTGGGTTTGGGTGCTTTTGGGTTTCAGCTGAAGGAAGCCCCTGCCGGGAAGCACATTATCACAACCACCAGATCCCATAGCAACCAGCTGGTCACAGACTGCATTTCCGCCATGAACCCCGACACCGTGCTGCGAGTGGGAGGAGCAGGAAACAAGACCTCGGCCACCGACCTGCAGACCAAAGCCGACCGCTTGGTGCAGATGAGCATATGCTCTTCCCTGGCCCGGAAGTTCCCGAAGCTGACCATCATAGGGGAAGAGGACCTGCCTCCTGGGGAAGTGGATCAAGAGCTGATTGAAGACGGGCAGTGGGAGGAGATCCTGAAGCAGCCGTGCCCCTCGCAGTACAGTGCTATCAAGGAGGAAGACCTTGTGGTTTGGGTTGACCCCTTAGATGGCACCAAGGAGTACACTGAAGGTCTTCTTGACAATGTAACAGTTCTCATTGGGATTGCTTATGAAGGAAAGGCCATCGCAGGCATCATCAACCAGCCGTATTACAACTACCAGGCAGGACCCGACGCGGCGCTGGGCAGGACCATCTGGGGAGTTCTGGGTTTGGGTGCTTTTGGGTTTCAGCTGAAGGAAGCCCCTGCCGGGAAGCACATTATCACAACCACCAGATCCCATAGCAACCAGCTGGTCACAGACTGCATTTCCGCCATGAACCCCGACACCGTGCTGCGAGTGGGAGGAGCAGGAAACAAG
Peptide sequence:
MSICSSLARKFPKLTIIGEEDLPPGEVDQELIEDGQWEEILKQPCPSQYSAIKEEDLVVWVDPLDGTKEYTEGLLDNVTVLIGIAYEGKAIAGIINQPYYNYQAGPDAALGRTIWGVLGLGAFGFQLKEAPAGKHIITTTRSHSNQLVTDCISAMNPDTVLRVGGAGNKTSATDLQTKADRLVQMSICSSLARKFPKLTIIGEEDLPPGEVDQELIEDGQWEEILKQPCPSQYSAIKEEDLVVWVDPLDGTKEYTEGLLDNVTVLIGIAYEGKAIAGIINQPYYNYQAGPDAALGRTIWGVLGLGAFGFQLKEAPAGKHIITTTRSHSNQLVTDCISAMNPDTVLRVGGAGNK
IRESfinder result
IRESfinder score
0.412570445
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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