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mmu_circBmpr2_453
Basic information of circular RNA
PanCircBase ID:
mmu_circBmpr2_453
mm39_Chr. Position ID:
chr1|59852746|59880700|+
Gene name:
Bmpr2
Transcript:
ENSMUST00000087435.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
2,3,4
Exon/intron offsets:
0,1653,27843
Genomic length:
27954
Exon/intron sizes in nt:
17,11,71,111
Flanking exon/introns:
chr1:59803887-59852746|chr1:59880700-59881901
Splice length:
453
Other database IDs:
circAtlas: mmu-Bmpr2_0017
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
CTTCTCAGAATCAAGAACGGCTGTGTGCATTTAAAGATCCATATCAACAAGATCTTGGGATAGGTGAGAGTCGAATCTCTCATGAAAATGGGACAATATTATGTTCCAAAGGGAGCACGTGTTATGGTCTGTGGGAGAAATCAAAAGGGGACATCAATCTTGTGAAACAAGGATGTTGGTCTCACATCGGTGATCCCCAAGAGTGCCACTATGAAGAGTGTGTAGTAACTACCACCCCACCCTCAATTCAGAATGGAACGTACCGCTTTTGCTGCTGTAGTACAGATTTATGTAATGTCAACTTTACTGAGAACTTTCCACCCCCTGACACAACACCACTCAGTCCACCTCATTCATTTAATCGAGATGAAACGATAATCATTGCTTTGGCATCAGTTTCTGTGTTAGCTGTTTTGATAGTCGCCTTATGTTTTGGATACAGAATGTTGACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCTTTGGCATCAGTTTCTGTG
59.97
TCGACTCTCACCTATCCCAAGA
59.76
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTTTGGCATCAGTTTCTGTGT
59.96
TCGACTCTCACCTATCCCAAGA
59.76
144
PCR template sequence:
TCATTTAATCGAGATGAAACGATAATCATTGCTTTGGCATCAGTTTCTGTGTTAGCTGTTTTGATAGTCGCCTTATGTTTTGGATACAGAATGTTGACAGCTTCTCAGAATCAAGAACGGCTGTGTGCATTTAAAGATCCATATCAACAAGATCTTGGGATAGGTGAGAGTCGAATCTCTCATGAAAATGGGACAATATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-15a-5p(1), mmu-miR-204-5p(1), mmu-miR-503-5p(1), mmu-miR-362-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGAATGTTGACAGCTTCTCAGAATCA
siRNA sets
ATTCTGAGAAGCTGTCAAC, TTCTGAGAAGCTGTCAACA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBMPR2_453_ORF_1
0.6947
-0.044216298
0.305922389
462
ORF sequence:
ATGTTGACAGCTTCTCAGAATCAAGAACGGCTGTGTGCATTTAAAGATCCATATCAACAAGATCTTGGGATAGGTGAGAGTCGAATCTCTCATGAAAATGGGACAATATTATGTTCCAAAGGGAGCACGTGTTATGGTCTGTGGGAGAAATCAAAAGGGGACATCAATCTTGTGAAACAAGGATGTTGGTCTCACATCGGTGATCCCCAAGAGTGCCACTATGAAGAGTGTGTAGTAACTACCACCCCACCCTCAATTCAGAATGGAACGTACCGCTTTTGCTGCTGTAGTACAGATTTATGTAATGTCAACTTTACTGAGAACTTTCCACCCCCTGACACAACACCACTCAGTCCACCTCATTCATTTAATCGAGATGAAACGATAATCATTGCTTTGGCATCAGTTTCTGTGTTAGCTGTTTTGATAGTCGCCTTATGTTTTGGATACAGAATGTTGACA
Peptide sequence:
MLTASQNQERLCAFKDPYQQDLGIGESRISHENGTILCSKGSTCYGLWEKSKGDINLVKQGCWSHIGDPQECHYEECVVTTTPPSIQNGTYRFCCCSTDLCNVNFTENFPPPDTTPLSPPHSFNRDETIIIALASVSVLAVLIVALCFGYRMLT
IRESfinder result
IRESfinder score
0.703281007
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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