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mmu_circBmp2k_705
Basic information of circular RNA
PanCircBase ID:
mmu_circBmp2k_705
mm39_Chr. Position ID:
chr5|97175689|97193026|+
Gene name:
Bmp2k
Transcript:
ENSMUST00000112974.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
2,3,4,5,6,7
Exon/intron offsets:
0,3306,9065,10590,12842,17204
Genomic length:
17337
Exon/intron sizes in nt:
11,91,06,14,31,22,82,100
Flanking exon/introns:
chr5:97145955-97175689|chr5:97193026-97200955
Splice length:
705
Other database IDs:
CIRCpedia: MMU_CIRCpedia_35490, circAtlas: mmu-Bmp2k_0003
circBase: hsa_circ_0070190, circAtlas: hsa-BMP2K_0004, CIRCpedia: HSA_CIRCpedia_49241
Human Ortholog IDs:
Mature spliced Sequence:
GTGGATTCTCCACTGTTTTCCTGGTGCGGACTCACAGTGGGATCCGATGTGCACTGAAGCGAATGTATGTCAATAACACACCCGACCTCAACATTTGTAAAAGGGAAATCACGATTATGAAAGAGTTATCTGGCCACAAAAATATTGTCGGTTATTTGGACTGTGCTGTCAATTCAATTAGTGACAATGTGTGGGAAGTGCTTATCTTAATGGAATATTGTCGAGCTGGGCAGGTTGTGAACCAGATGAATAAGAAGCTGCAGACAGGCTTTACCGAGTCAGAAGTCCTACAAATCTTCTGTGATACCTGCGAAGCTGTCGCACGATTGCATCAATGTAAGACTCCCATAATCCACCGGGATCTGAAGGTAGAAAATATTTTGCTAAATGATGCTGGAAATTATGTGCTTTGTGACTTTGGCAGTGCCACTAATAAATTTCTCAATCCTCAAAAAGATGGAGTTAATGTAGTAGAAGAAGAAATTAAAAAATACACGACTCTGTCGTATAGAGCACCAGAAATGATCAACCTTTATGGAGGGAAACCGATCACCACCAAGGCTGATATCTGGGCCTTGGGATGTTTACTGTATAAACTTTGTTTCTTCACTCTTCCTTTTGGTGAGAGTCAGGTTGCTATCTGTGATGGCAGCTTCACCATCCCAGACAATTCTCGCTACTCCCACAACGTGCATTGCTTGATTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGTGATGGCAGCTTCACC
60.32
ATGTTGAGGTCGGGTGTGTT
59.53
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGTGATGGCAGCTTCACC
60.32
AATGTTGAGGTCGGGTGTGT
59.53
161
PCR template sequence:
TTCACTCTTCCTTTTGGTGAGAGTCAGGTTGCTATCTGTGATGGCAGCTTCACCATCCCAGACAATTCTCGCTACTCCCACAACGTGCATTGCTTGATTAGTGGATTCTCCACTGTTTTCCTGGTGCGGACTCACAGTGGGATCCGATGTGCACTGAAGCGAATGTATGTCAATAACACACCCGACCTCAACATTTGTAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-30e-5p(1), mmu-miR-30a-5p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-30c-5p(1), mmu-miR-30d-5p(1), mmu-miR-30b-5p(1), mmu-miR-136-5p(1), mmu-miR-127-3p(1), mmu-miR-22-3p(1), mmu-miR-539-5p(1), mmu-miR-350-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CATTGCTTGATTAGTGGATTCTCCAC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBMP2K_705_ORF_1
0.8787
0.124609711
0.998624913
1347
ORF sequence:
ATGTATGTCAATAACACACCCGACCTCAACATTTGTAAAAGGGAAATCACGATTATGAAAGAGTTATCTGGCCACAAAAATATTGTCGGTTATTTGGACTGTGCTGTCAATTCAATTAGTGACAATGTGTGGGAAGTGCTTATCTTAATGGAATATTGTCGAGCTGGGCAGGTTGTGAACCAGATGAATAAGAAGCTGCAGACAGGCTTTACCGAGTCAGAAGTCCTACAAATCTTCTGTGATACCTGCGAAGCTGTCGCACGATTGCATCAATGTAAGACTCCCATAATCCACCGGGATCTGAAGGTAGAAAATATTTTGCTAAATGATGCTGGAAATTATGTGCTTTGTGACTTTGGCAGTGCCACTAATAAATTTCTCAATCCTCAAAAAGATGGAGTTAATGTAGTAGAAGAAGAAATTAAAAAATACACGACTCTGTCGTATAGAGCACCAGAAATGATCAACCTTTATGGAGGGAAACCGATCACCACCAAGGCTGATATCTGGGCCTTGGGATGTTTACTGTATAAACTTTGTTTCTTCACTCTTCCTTTTGGTGAGAGTCAGGTTGCTATCTGTGATGGCAGCTTCACCATCCCAGACAATTCTCGCTACTCCCACAACGTGCATTGCTTGATTAGTGGATTCTCCACTGTTTTCCTGGTGCGGACTCACAGTGGGATCCGATGTGCACTGAAGCGAATGTATGTCAATAACACACCCGACCTCAACATTTGTAAAAGGGAAATCACGATTATGAAAGAGTTATCTGGCCACAAAAATATTGTCGGTTATTTGGACTGTGCTGTCAATTCAATTAGTGACAATGTGTGGGAAGTGCTTATCTTAATGGAATATTGTCGAGCTGGGCAGGTTGTGAACCAGATGAATAAGAAGCTGCAGACAGGCTTTACCGAGTCAGAAGTCCTACAAATCTTCTGTGATACCTGCGAAGCTGTCGCACGATTGCATCAATGTAAGACTCCCATAATCCACCGGGATCTGAAGGTAGAAAATATTTTGCTAAATGATGCTGGAAATTATGTGCTTTGTGACTTTGGCAGTGCCACTAATAAATTTCTCAATCCTCAAAAAGATGGAGTTAATGTAGTAGAAGAAGAAATTAAAAAATACACGACTCTGTCGTATAGAGCACCAGAAATGATCAACCTTTATGGAGGGAAACCGATCACCACCAAGGCTGATATCTGGGCCTTGGGATGTTTACTGTATAAACTTTGTTTCTTCACTCTTCCTTTTGGTGAGAGTCAGGTTGCTATCTGTGATGGCAGCTTCACCATCCCAGACAATTCTCGCTACTCCCACAACGTGCATTGCTTGATT
Peptide sequence:
MYVNNTPDLNICKREITIMKELSGHKNIVGYLDCAVNSISDNVWEVLILMEYCRAGQVVNQMNKKLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLNDAGNYVLCDFGSATNKFLNPQKDGVNVVEEEIKKYTTLSYRAPEMINLYGGKPITTKADIWALGCLLYKLCFFTLPFGESQVAICDGSFTIPDNSRYSHNVHCLISGFSTVFLVRTHSGIRCALKRMYVNNTPDLNICKREITIMKELSGHKNIVGYLDCAVNSISDNVWEVLILMEYCRAGQVVNQMNKKLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLNDAGNYVLCDFGSATNKFLNPQKDGVNVVEEEIKKYTTLSYRAPEMINLYGGKPITTKADIWALGCLLYKLCFFTLPFGESQVAICDGSFTIPDNSRYSHNVHCLI
IRESfinder result
IRESfinder score
0.657625217
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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