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mmu_circBirc6_926
Basic information of circular RNA
PanCircBase ID:
mmu_circBirc6_926
mm39_Chr. Position ID:
chr17|74933956|74945465|+
Gene name:
Birc6
Transcript:
ENSMUST00000180037.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
38,39,40,41,42,43,44
Exon/intron offsets:
0,2443,3998,4649,7834,8801,11381
Genomic length:
11509
Exon/intron sizes in nt:
15,41,17,15,51,27,11,10,00,000
Flanking exon/introns:
chr17:74932234-74933956|chr17:74945465-74946774
Splice length:
926
Other database IDs:
circAtlas: mmu-Birc6_0047
circAtlas: hsa-BIRC6_0133, CIRCpedia: HSA_CIRCpedia_134066
Human Ortholog IDs:
Mature spliced Sequence:
CCTATAAGCAGTACATGGTATGACTACTGGGGTGCTGATTATGGTACCTATAATTATAATCCTTACATTGGAGGCCTGGGGATGCCTGTAGCAAAACCACCATCAAACACAGAGAAGAATGGATCCCAGACTGTCAGTGTTTCGGTGTCTCAGGCTTTAGATGCTCGTCTAGAAGTTGGTCTTGAACAGCAAGCAGAACTTATGTTGAAAATGATGTCTACTCTGGAAGCAGACTCCATCTTACAAGCATTAACAAATACATCTCCTACCTTTTCACAATCTCCAACTGGAACAGATGACTCACTTCTGGGGAATTTACAGCCAGCAAACCAGAACAGCCAGCTCATGATACAGCTCTCATCTGTCCCAATGTTAAATGTTTGTTTCAATAAACTCTTCTCCATGCTTCAAGTCCATCATGTTCAGTTGGAGTCACTTCTTCAGTTGTGGCTCACATTGAGCCTTAATTCTAGTTCATCAGGGAACAAAGAGAATGGAGCAGATATTTTTTTATATAATGCTAATCGGATACCTGTCATCTCATTAAATCAAGCATCAATAGCTAGCTTTCTCACAGTGCTAGCTTGGTATCCAAATACTTTGCTCCGGACGTGGTGCCTTGTGCTTCATAGCTTAACACTCATGACGAATATGCAGCTTAATTCTGGTTCCAGCAGTTCTATTGGAATTCAGGAGACTACGGCTCATCTGTTGGTTTCAGATCCAAACCTTATTCATGTGTTAGTGAAATTTCTTTCTGGTACCAGTCCTCATGGGACGAATCAGCATAGCCCACAGGTTGGTCCAACAGCTACACAAGCCATGCAGGAATTTCTCACCCGACTACAAGTGCATTTGTCTTCAACATGTCCCCAGATATTCAGTGAACTTTTGCTCAAGTTAATTCACATACTGTCCACAGAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCACCCGACTACAAGTGCA
59.68
CCCAGGCCTCCAATGTAAGG
60.11
172
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCACCCGACTACAAGTGCA
59.68
CCCCAGGCCTCCAATGTAAG
60.11
173
PCR template sequence:
AGGAATTTCTCACCCGACTACAAGTGCATTTGTCTTCAACATGTCCCCAGATATTCAGTGAACTTTTGCTCAAGTTAATTCACATACTGTCCACAGAAAGCCTATAAGCAGTACATGGTATGACTACTGGGGTGCTGATTATGGTACCTATAATTATAATCCTTACATTGGAGGCCTGGGGATGCCTGTAGCAAAACCAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-155-5p(1), mmu-miR-200a-3p(1), mmu-let-7e-5p(1), mmu-miR-324-5p(1), mmu-miR-495-3p(1), mmu-miR-136-5p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-541-5p(1), mmu-miR-539-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTCCACAGAAAGCCTATAAGCAGTA
siRNA sets
ACTGCTTATAGGCTTTCTG, TACTGCTTATAGGCTTTCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBIRC6_926_ORF_1
0.9311
-0.076277177
0.907339413
852
ORF sequence:
ATGCCTGTAGCAAAACCACCATCAAACACAGAGAAGAATGGATCCCAGACTGTCAGTGTTTCGGTGTCTCAGGCTTTAGATGCTCGTCTAGAAGTTGGTCTTGAACAGCAAGCAGAACTTATGTTGAAAATGATGTCTACTCTGGAAGCAGACTCCATCTTACAAGCATTAACAAATACATCTCCTACCTTTTCACAATCTCCAACTGGAACAGATGACTCACTTCTGGGGAATTTACAGCCAGCAAACCAGAACAGCCAGCTCATGATACAGCTCTCATCTGTCCCAATGTTAAATGTTTGTTTCAATAAACTCTTCTCCATGCTTCAAGTCCATCATGTTCAGTTGGAGTCACTTCTTCAGTTGTGGCTCACATTGAGCCTTAATTCTAGTTCATCAGGGAACAAAGAGAATGGAGCAGATATTTTTTTATATAATGCTAATCGGATACCTGTCATCTCATTAAATCAAGCATCAATAGCTAGCTTTCTCACAGTGCTAGCTTGGTATCCAAATACTTTGCTCCGGACGTGGTGCCTTGTGCTTCATAGCTTAACACTCATGACGAATATGCAGCTTAATTCTGGTTCCAGCAGTTCTATTGGAATTCAGGAGACTACGGCTCATCTGTTGGTTTCAGATCCAAACCTTATTCATGTGTTAGTGAAATTTCTTTCTGGTACCAGTCCTCATGGGACGAATCAGCATAGCCCACAGGTTGGTCCAACAGCTACACAAGCCATGCAGGAATTTCTCACCCGACTACAAGTGCATTTGTCTTCAACATGTCCCCAGATATTCAGTGAACTTTTGCTCAAGTTAATTCACATACTGTCCACAGAAAGCCTATAA
Peptide sequence:
MPVAKPPSNTEKNGSQTVSVSVSQALDARLEVGLEQQAELMLKMMSTLEADSILQALTNTSPTFSQSPTGTDDSLLGNLQPANQNSQLMIQLSSVPMLNVCFNKLFSMLQVHHVQLESLLQLWLTLSLNSSSSGNKENGADIFLYNANRIPVISLNQASIASFLTVLAWYPNTLLRTWCLVLHSLTLMTNMQLNSGSSSSIGIQETTAHLLVSDPNLIHVLVKFLSGTSPHGTNQHSPQVGPTATQAMQEFLTRLQVHLSSTCPQIFSELLLKLIHILSTESL
IRESfinder result
IRESfinder score
0.757079606
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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