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mmu_circBirc6_840
Basic information of circular RNA
PanCircBase ID:
mmu_circBirc6_840
mm39_Chr. Position ID:
chr17|74901864|74906962|+
Gene name:
Birc6
Transcript:
ENSMUST00000180037.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
14,15,16,17,18,19
Exon/intron offsets:
0,1987,3169,4113,4367,5000
Genomic length:
5098
Exon/intron sizes in nt:
132,182,134,162,132,98
Flanking exon/introns:
chr17:74901448-74901864|chr17:74906962-74909959
Splice length:
840
Other database IDs:
circAtlas: mmu-Birc6_0123
Human Ortholog IDs:
Mature spliced Sequence:
GTCTTAGGTTATGTCCATTCTTAGAGGATCATAAAGAAGACATTCTGTGTGGCCCAGTATGGCTTGCTAGTGGTCTGGACCTATCAGGTCATGCTGGGATGTTGACGTTGACAAGCCCCAAACTTGTTAAAGGTATGGCAGGAGGGAAGTACCGTTCATTTTTAATTCATGTCAAAGCAGTGAGTGACCGAGGAGCAGCAGATGAGATGTGCAGCAGTGGCCTGCGTCCTGTTGTGAGGCTTCCATCTCTTAAACAGCAGGGTCACAAGGGATACTCACTGGCTTCTCTCTTGGCCAAAGTTGCAGCAGGCAAGGAAAAATCATCTAATGTTAAAAATGAAAATGCAGGAGGCACCCGAAAGTCTGAAAACCTGCGGGGTTGTGATTTACTTCAGGAGGTGTCTGTCACCATTCGGAGGTTTAAGAAAACCTCCATCTGTAAGGAAAGGGTTCAGCGTTGCGCCATGTTACAGTTTTCAGAATTTCATGAGAAGCTTCTTAACACGCTTTGTAGACGATCAGATGATGGGCAGGTCACAGAGCACGCCCAGAGTCTTGTGTTGGATGCTCTCTGCTGGTTAGCAGGAGTCCATTCAAATGGATCTGGCAGCTCAAAGGAAGGAAATGAGTGCCTACTTTCAAAAACACGGAAATGTCTTTCCGATATTGTCCGTGTATGCTTCTTTGAGGCAGGACGAAGTATAGCCCATAAATGTGCTCGGTTTCTAGCCTTGTGCATTAGTAATGGCAAATGCGAGCCCTGTCAGCCTGGATTTGGATCGGTTCTATTGAAGGCTTTACTTGATAATATGTGCTTTTTACCAGCAGCAGCAACTGGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCCTGGATTTGGATCGGT
60.11
CCAGACCACTAGCAAGCCAT
59.75
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGCCTGGATTTGGATCGG
59.82
CCAGACCACTAGCAAGCCAT
59.75
155
PCR template sequence:
AGTAATGGCAAATGCGAGCCCTGTCAGCCTGGATTTGGATCGGTTCTATTGAAGGCTTTACTTGATAATATGTGCTTTTTACCAGCAGCAGCAACTGGTGGTCTTAGGTTATGTCCATTCTTAGAGGATCATAAAGAAGACATTCTGTGTGGCCCAGTATGGCTTGCTAGTGGTCTGGACCTATCAGGTCATGCTGGGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-26a-5p(1), mmu-miR-26b-5p(1), mmu-miR-22-3p(1), mmu-miR-690(1), mmu-miR-335-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCAGCAACTGGTGGTCTTAGGTTATG
siRNA sets
ATAACCTAAGACCACCAGT, CATAACCTAAGACCACCAG, AACCTAAGACCACCAGTTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBIRC6_840_ORF_1
0.673
0.020782814
0.999426472
1581
ORF sequence:
ATGTTGACGTTGACAAGCCCCAAACTTGTTAAAGGTATGGCAGGAGGGAAGTACCGTTCATTTTTAATTCATGTCAAAGCAGTGAGTGACCGAGGAGCAGCAGATGAGATGTGCAGCAGTGGCCTGCGTCCTGTTGTGAGGCTTCCATCTCTTAAACAGCAGGGTCACAAGGGATACTCACTGGCTTCTCTCTTGGCCAAAGTTGCAGCAGGCAAGGAAAAATCATCTAATGTTAAAAATGAAAATGCAGGAGGCACCCGAAAGTCTGAAAACCTGCGGGGTTGTGATTTACTTCAGGAGGTGTCTGTCACCATTCGGAGGTTTAAGAAAACCTCCATCTGTAAGGAAAGGGTTCAGCGTTGCGCCATGTTACAGTTTTCAGAATTTCATGAGAAGCTTCTTAACACGCTTTGTAGACGATCAGATGATGGGCAGGTCACAGAGCACGCCCAGAGTCTTGTGTTGGATGCTCTCTGCTGGTTAGCAGGAGTCCATTCAAATGGATCTGGCAGCTCAAAGGAAGGAAATGAGTGCCTACTTTCAAAAACACGGAAATGTCTTTCCGATATTGTCCGTGTATGCTTCTTTGAGGCAGGACGAAGTATAGCCCATAAATGTGCTCGGTTTCTAGCCTTGTGCATTAGTAATGGCAAATGCGAGCCCTGTCAGCCTGGATTTGGATCGGTTCTATTGAAGGCTTTACTTGATAATATGTGCTTTTTACCAGCAGCAGCAACTGGTGGTCTTAGGTTATGTCCATTCTTAGAGGATCATAAAGAAGACATTCTGTGTGGCCCAGTATGGCTTGCTAGTGGTCTGGACCTATCAGGTCATGCTGGGATGTTGACGTTGACAAGCCCCAAACTTGTTAAAGGTATGGCAGGAGGGAAGTACCGTTCATTTTTAATTCATGTCAAAGCAGTGAGTGACCGAGGAGCAGCAGATGAGATGTGCAGCAGTGGCCTGCGTCCTGTTGTGAGGCTTCCATCTCTTAAACAGCAGGGTCACAAGGGATACTCACTGGCTTCTCTCTTGGCCAAAGTTGCAGCAGGCAAGGAAAAATCATCTAATGTTAAAAATGAAAATGCAGGAGGCACCCGAAAGTCTGAAAACCTGCGGGGTTGTGATTTACTTCAGGAGGTGTCTGTCACCATTCGGAGGTTTAAGAAAACCTCCATCTGTAAGGAAAGGGTTCAGCGTTGCGCCATGTTACAGTTTTCAGAATTTCATGAGAAGCTTCTTAACACGCTTTGTAGACGATCAGATGATGGGCAGGTCACAGAGCACGCCCAGAGTCTTGTGTTGGATGCTCTCTGCTGGTTAGCAGGAGTCCATTCAAATGGATCTGGCAGCTCAAAGGAAGGAAATGAGTGCCTACTTTCAAAAACACGGAAATGTCTTTCCGATATTGTCCGTGTATGCTTCTTTGAGGCAGGACGAAGTATAGCCCATAAATGTGCTCGGTTTCTAGCCTTGTGCATTAGTAATGGCAAATGCGAGCCCTGTCAGCCTGGATTTGGATCGGTTCTATTGAAGGCTTTACTTGATAATATGTGCTTTTTACCAGCAGCAGCAACTGGT
Peptide sequence:
MLTLTSPKLVKGMAGGKYRSFLIHVKAVSDRGAADEMCSSGLRPVVRLPSLKQQGHKGYSLASLLAKVAAGKEKSSNVKNENAGGTRKSENLRGCDLLQEVSVTIRRFKKTSICKERVQRCAMLQFSEFHEKLLNTLCRRSDDGQVTEHAQSLVLDALCWLAGVHSNGSGSSKEGNECLLSKTRKCLSDIVRVCFFEAGRSIAHKCARFLALCISNGKCEPCQPGFGSVLLKALLDNMCFLPAAATGGLRLCPFLEDHKEDILCGPVWLASGLDLSGHAGMLTLTSPKLVKGMAGGKYRSFLIHVKAVSDRGAADEMCSSGLRPVVRLPSLKQQGHKGYSLASLLAKVAAGKEKSSNVKNENAGGTRKSENLRGCDLLQEVSVTIRRFKKTSICKERVQRCAMLQFSEFHEKLLNTLCRRSDDGQVTEHAQSLVLDALCWLAGVHSNGSGSSKEGNECLLSKTRKCLSDIVRVCFFEAGRSIAHKCARFLALCISNGKCEPCQPGFGSVLLKALLDNMCFLPAAATG
IRESfinder result
IRESfinder score
0.635380603
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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