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mmu_circBirc6_444
Basic information of circular RNA
PanCircBase ID:
mmu_circBirc6_444
mm39_Chr. Position ID:
chr17|74930745|74934110|+
Gene name:
Birc6
Transcript:
ENSMUST00000180037.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
36,37,38
Exon/intron offsets:
0,1355,3211
Genomic length:
3365
Exon/intron sizes in nt:
15,61,34,154
Flanking exon/introns:
chr17:74930087-74930745|chr17:74934110-74936399
Splice length:
444
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_134065
Human Ortholog IDs:
Mature spliced Sequence:
GAGAATTGTTGGCTCCGGTGGCTGCAGAAGCGATGGAAGAATGCACTGTGAGTGAGGATGTTGGTGCAACAGCTGGCGACTCTGATGACTCCCTGCAGCAGTCCCCCGCTCAGCTGCTGGAAACCATAGATGAACCATTGACACATGAGATAGCAGGTACGCCTCCTCTCTCTTCCTTGGAAAAAGATAAGGAAATTGACCTTGAGCTACTTCAAGACCTCATGGAAGTTGACATTGATCCTTTAGATATTGATCTAGAAAAGGATCCTCTTGCAGCCAAAGTTTTTAAGCCTATAAGCAGTACATGGTATGACTACTGGGGTGCTGATTATGGTACCTATAATTATAATCCTTACATTGGAGGCCTGGGGATGCCTGTAGCAAAACCACCATCAAACACAGAGAAGAATGGATCCCAGACTGTCAGTGTTTCGGTGTCTCAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTTACATTGGAGGCCTGGGG
60.11
ATCAGAGTCGCCAGCTGTTG
60.39
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTTACATTGGAGGCCTGGGG
60.11
CATCAGAGTCGCCAGCTGTT
60.39
180
PCR template sequence:
TATAATCCTTACATTGGAGGCCTGGGGATGCCTGTAGCAAAACCACCATCAAACACAGAGAAGAATGGATCCCAGACTGTCAGTGTTTCGGTGTCTCAGGGAGAATTGTTGGCTCCGGTGGCTGCAGAAGCGATGGAAGAATGCACTGTGAGTGAGGATGTTGGTGCAACAGCTGGCGACTCTGATGACTCCCTGCAGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-324-5p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-541-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-3105-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCGGTGTCTCAGGGAGAATTGTTGGC
siRNA sets
CAATTCTCCCTGAGACACC, ATTCTCCCTGAGACACCGA, CAACAATTCTCCCTGAGAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBIRC6_444_ORF_1
1.1943
0.210500617
0.981795631
855
ORF sequence:
ATGGAAGAATGCACTGTGAGTGAGGATGTTGGTGCAACAGCTGGCGACTCTGATGACTCCCTGCAGCAGTCCCCCGCTCAGCTGCTGGAAACCATAGATGAACCATTGACACATGAGATAGCAGGTACGCCTCCTCTCTCTTCCTTGGAAAAAGATAAGGAAATTGACCTTGAGCTACTTCAAGACCTCATGGAAGTTGACATTGATCCTTTAGATATTGATCTAGAAAAGGATCCTCTTGCAGCCAAAGTTTTTAAGCCTATAAGCAGTACATGGTATGACTACTGGGGTGCTGATTATGGTACCTATAATTATAATCCTTACATTGGAGGCCTGGGGATGCCTGTAGCAAAACCACCATCAAACACAGAGAAGAATGGATCCCAGACTGTCAGTGTTTCGGTGTCTCAGGGAGAATTGTTGGCTCCGGTGGCTGCAGAAGCGATGGAAGAATGCACTGTGAGTGAGGATGTTGGTGCAACAGCTGGCGACTCTGATGACTCCCTGCAGCAGTCCCCCGCTCAGCTGCTGGAAACCATAGATGAACCATTGACACATGAGATAGCAGGTACGCCTCCTCTCTCTTCCTTGGAAAAAGATAAGGAAATTGACCTTGAGCTACTTCAAGACCTCATGGAAGTTGACATTGATCCTTTAGATATTGATCTAGAAAAGGATCCTCTTGCAGCCAAAGTTTTTAAGCCTATAAGCAGTACATGGTATGACTACTGGGGTGCTGATTATGGTACCTATAATTATAATCCTTACATTGGAGGCCTGGGGATGCCTGTAGCAAAACCACCATCAAACACAGAGAAGAATGGATCCCAGACTGTCAGTGTTTCGGTGTCTCAG
Peptide sequence:
MEECTVSEDVGATAGDSDDSLQQSPAQLLETIDEPLTHEIAGTPPLSSLEKDKEIDLELLQDLMEVDIDPLDIDLEKDPLAAKVFKPISSTWYDYWGADYGTYNYNPYIGGLGMPVAKPPSNTEKNGSQTVSVSVSQGELLAPVAAEAMEECTVSEDVGATAGDSDDSLQQSPAQLLETIDEPLTHEIAGTPPLSSLEKDKEIDLELLQDLMEVDIDPLDIDLEKDPLAAKVFKPISSTWYDYWGADYGTYNYNPYIGGLGMPVAKPPSNTEKNGSQTVSVSVSQ
IRESfinder result
IRESfinder score
0.518760397
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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